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Method of culturing bacillus licheniformis to prepare animal feed by using distiller grains and extruded maize meal

A technology of Bacillus licheniformis and puffed corn flour, applied in animal feed, animal feed, microorganism-based methods, etc., can solve the problems of insufficient animal nutrition supply, affecting animal growth, limited increase in protein content, etc., and improve the antibody level of the body. , the effect of improving digestion and utilization rate and reducing breeding cost

Active Publication Date: 2019-10-29
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, distiller's grains have been used for animal feed after solid-state fermentation. However, the protein content in the fermented material is limited. When fed as animal feed alone, it will still lead to insufficient animal nutrition supply and affect the technical problems of animal growth. Therefore, it is often necessary to mix and feed other high-protein raw materials to meet the nutritional needs of animals for growth. How to further increase the nutrient content in fermented distiller's grains so that they can meet the nutrient requirements necessary for animal growth when fed alone important meaning

Method used

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  • Method of culturing bacillus licheniformis to prepare animal feed by using distiller grains and extruded maize meal
  • Method of culturing bacillus licheniformis to prepare animal feed by using distiller grains and extruded maize meal
  • Method of culturing bacillus licheniformis to prepare animal feed by using distiller grains and extruded maize meal

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Experimental program
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Effect test

Embodiment 1

[0036] (1) Preparation of Bacillus licheniformis seed liquid:

[0037] The lyophilized powder of Bacillus licheniformis was inoculated into the slant medium of potato medium for activation culture, and then transferred to liquid medium for cultivation. The number of viable bacteria detected was not less than 1×10 9 After cfu / mL, it was used as Bacillus licheniformis seed solution;

[0038]Liquid medium: 200g of potatoes, cut into pieces, add 1000ml of water, boil for 30min, filter through gauze, add 20g of sucrose, add water to 1000ml, adjust the pH value to 7.0, and autoclave;

[0039] (2) Preparation of fermented raw materials: mix distiller's grains and puffed corn flour evenly with a mass ratio of 8:2, and then adjust the pH to 7.0 with calcium oxide;

[0040] (3) Fermentation culture: Add the Bacillus licheniformis seed liquid prepared in step (1) into the fermentation raw materials, mix and spread it in the fermentation tank, the thickness of the laying is 4-10cm, and c...

Embodiment 2

[0043] The steps are the same as in the embodiment (1), except that the puffed corn flour in the fermentation raw material is replaced with corn flour to prepare the feed.

Embodiment 3

[0045] The steps are the same as in Example (1), except that 10 g of distiller's grains and 2 g of puffed corn flour are added to the liquid medium to prepare the feed.

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Abstract

The invention discloses a method and application of culturing bacillus licheniformis to prepare animal feed by using distiller grains and extruded maize meal. The method includes the following steps that solid byproduct distiller grains fermenting white wine are taken as raw materials, the extruded maize meal is added, activated bacillus licheniformis is used as a seed solution to conduct solid-state fermentation, through microbial fermentation, the protein content of the distiller grains can be improved, the cellulose content is reduced, through the degradation of anti-nutrient substances, the nutritive value and palatability of the distiller grains are improved, spores of the bacillus licheniformis in final fermentation products can reach 1*10<9> cfu / mL (wet weight), the bacillus is distributed in feed in a form of endospore, after the spores enter into animal intestines and stomach, a large number of digestive enzymes are secreted, the ability of animals to digest vegetable proteins, fat, starch and other complex compounds is enhanced, intestinal gurgling is stimulated, the health of intestinal microorganisms is strengthened, the growth and development of immune organs are promoted, the level of body antibodies is improved, the growth of the animals is promoted, the breeding cost is lowered, and the efficiency is increased.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for cultivating bacillus licheniformis by using distiller's grains and puffed corn flour to prepare animal feed. Background technique [0002] Distiller's grains are the main by-product produced in the brewing process, and the price is low. my country is a big producer of liquor, with an annual output of more than 27 million tons of various distiller's grains, and the supply is very sufficient. Due to the high water content, high acidity and strong viscosity of distiller's grains, it is easy to spoil and deteriorate, it is not easy to store, and the BOD value is extremely high. If it is directly discharged into the river without treatment, it will not only waste precious resources, but also cause serious environmental pollution. The main components of distiller's grains are protein, cellulose, fat, vitamins and various trace elements, so liquor production enterpris...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/38A23K10/30A23K20/24A23K20/26A23K20/20C12N1/20C12R1/10
CPCA23K10/12A23K10/30A23K10/38A23K20/24A23K20/26A23K20/30C12N1/20Y02P60/87
Inventor 彭楠常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV
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