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Cornu cervi pantotrichum liposome and preparation and application

A liposome and deer antler technology, applied in the biological field, can solve problems such as inability to concentrate and memory loss, achieve good application prospects, mild conditions, and improve memory reproduction ability.

Inactive Publication Date: 2019-10-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Excessive drinking of ethanol at one time will cause the central nervous system to change from excitement to inhibition, resulting in memory loss, inability to concentrate and other symptoms

Method used

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  • Cornu cervi pantotrichum liposome and preparation and application
  • Cornu cervi pantotrichum liposome and preparation and application
  • Cornu cervi pantotrichum liposome and preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Freeze-dry fresh velvet antler and crush it, add deionized water 20 times the mass of velvet antler, stir evenly, add bromelain with 0.5% (W / W) mass of velvet antler, and enzymolyze it at 50°C for 3 hours; pour it over after enzymatic hydrolysis The extract and the residue were added with equal volume of deionized water and 0.5% neutral protease of velvet antler mass, and enzymatically hydrolyzed at 50°C for 2 hours. After the reaction, the extracts were combined, and the temperature was raised to 90°C and kept for 15 minutes.

[0020] The enzymolysis solution was cooled to room temperature, centrifuged at a centrifugal force of 5000g for 10 minutes, and the supernatant was collected. The supernatant is suction-filtered through 300-mesh diatomaceous earth, and the filtrate is freeze-dried to obtain antler extract.

[0021] Add a certain amount of lecithin and cholesterol to the round bottom flask, add 50mL of methanol to make the concentrations of lecithin and cholester...

Embodiment 2

[0048] Freeze-dry the fresh velvet antler and crush it, add deionized water 20 times the mass of the velvet antler, stir evenly, add pepsin with 1% of the mass of the velvet antler (W / W), enzymolyze it at 37°C for 2 hours; pour it after the enzymolysis Add an equal volume of deionized water and 1% velvet mass trypsin to the extract and residue, and enzymolyze it at 55°C for 4 hours. After the reaction, combine the extracts, raise the temperature to 90°C and keep it warm for 10 minutes.

[0049] The enzymolysis solution was cooled to room temperature, centrifuged at a centrifugal force of 3000g for 20 minutes, and the supernatant was collected. The supernatant is suction-filtered through 300-mesh diatomaceous earth, and the filtrate is freeze-dried to obtain antler extract.

[0050] Add a certain amount of lecithin and cholesterol to the round bottom flask, add 75mL of methanol to make the concentrations of lecithin and cholesterol respectively 8mg / mL and 1.4mg / mL, remove the o...

Embodiment 3

[0052] Fresh velvet antler is freeze-dried and crushed, add deionized water 25 times the mass of velvet antler, stir evenly, add pepsin with 2% (W / W) mass of velvet antler, enzymatically hydrolyze at 37°C for 3 hours; after enzymatic hydrolysis, pour and extract Add an equal volume of deionized water and 0.5% velvet mass flavor protease to the residue, and enzymolyze it at 60°C for 2 hours. After the reaction, combine the extracts, raise the temperature to 98°C and keep it warm for 10 minutes.

[0053] The enzymolysis solution was cooled to room temperature, centrifuged at a centrifugal force of 10000g for 10 minutes, and the supernatant was collected. The supernatant is suction-filtered through 500-mesh diatomaceous earth, and the filtrate is freeze-dried to obtain antler extract.

[0054] Add a certain amount of lecithin and cholesterol to the round bottom flask, add 75mL of methanol to make the concentrations of lecithin and cholesterol respectively 10mg / mL and 2mg / mL, remo...

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Abstract

The invention relates to a preparation method and application of a cornu cervi pantotrichum liposome. The preparation method of the cornu cervi pantotrichum liposome is established, and the prepared cornu cervi pantotrichum liposome can improve memory reappearance ability of mice with memory reproduction disorders, has potential anti-Alzheimer's disease activity, can be applied to prevention and treatment of cognitive disorder diseases such as the Alzheimer's disease, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to velvet liposome, in particular to a preparation method and application of velvet liposome. Background technique [0002] Antler is the unossified and densely hairy young horns of the sika deer or red deer, an animal of the chordate phylum Mammalia deer family. Antler has been used as medicine for more than 2,000 years. "Compendium of Materia Medica" records that velvet "regenerates essence and nourishes marrow, nourishes blood and nourishes yang, strengthens muscles and bones, and cures all deafness, deafness, dark eyes, dizziness and dysentery". In recent years, studies have found that deer antler has various biological functions such as promoting nerve growth, immune regulation, anti-inflammation, anti-oxidation, and anti-aging. Antler contains proteins, glycosaminoglycans, phospholipids, fatty acids, hormone-like substances, nucleic acids, polyamines and various inorganic substance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/32A61K9/127A61P25/00A61P25/28A23L33/10A23L33/115
CPCA61K35/32A61K9/127A61P25/00A61P25/28A61K47/28A23L33/10A23L33/115A23V2002/00
Inventor 靳艳叶明亮晏嘉泽
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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