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Cornu cervi glycopeptide and preparation method and application

A technology of velvet sugar and velvet sugar, which is applied in the field of preparation of velvet glycopeptides, can solve problems such as inability to concentrate and memory loss, and achieve good application prospects, mild conditions, and high extraction rates

Inactive Publication Date: 2019-10-25
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Excessive drinking of ethanol at one time will cause the central nervous system to change from excitement to inhibition, resulting in memory loss, inability to concentrate and other symptoms

Method used

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  • Cornu cervi glycopeptide and preparation method and application
  • Cornu cervi glycopeptide and preparation method and application
  • Cornu cervi glycopeptide and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Freeze-dry 40g of fresh sika velvet velvet and crush it, add deionized water 20 times the mass of velvet antler, adjust the pH to 4.0 with 1M HCl, stir well, add pepsin with 0.1% (W / W) mass of velvet antler, and heat at 45°C Enzymolysis at high temperature for 3 hours; after the enzymolysis, adjust the pH to 7 with 4M NaOH, then add trypsin with 0.1% antler mass, and enzymolysis at 40°C for 3 hours (the optimal range is 2 to 10 hours) After the reaction, the temperature was raised to 90° C. and kept for 10 minutes. Cool the enzymatic solution to room temperature, centrifuge at 10,000g for 10 minutes, and collect the supernatant; measure the peptide concentration with Nanodrop Onec, dilute with water to a peptide concentration of 20 mg / mL for use; dilute the swollen Sephadex G-25 filler Pack into a gel column with a diameter of 10.0 cm and a column height of 90 cm. Load the enzymatic hydrolysis solution on the gel column, use deionized water as the eluent, and carry out...

Embodiment 2

[0052] Freeze-dry 40g of fresh sika velvet antler and crush it, add deionized water 30 times the mass of velvet antler, adjust the pH to 4.0 with 2M HCl, stir well, add papain with 1% (W / W) mass of velvet antler, and heat at 37°C Enzymolysis at high temperature for 2 hours; after the end of the enzymolysis, use 4M NaOH to adjust the pH to 6, then add chymotrypsin with 2% antler mass, and enzymolysis at 45°C for 4 hours, and then raise the temperature to 90°C and keep warm for 15 minutes.

[0053] The enzymolysis solution was cooled to room temperature, centrifuged at a speed of 15000 g for 12 minutes, and the supernatant was collected. The peptide concentration of the supernatant was determined by NanodropOnec, diluted with water to a peptide concentration of 20 mg / mL for use. Pack the swollen Sephadex G-25 filler into a gel column with a diameter of 5 cm and a column height of 40 cm. Load the enzymolysis solution on the gel column, use deionized water as the eluent, and car...

Embodiment 3

[0056] Freeze-dry 40g of fresh sika velvet velvet and crush it, add deionized water 25 times the mass of velvet antler, adjust the pH to 3.0 with 2M HCl, stir well, add bromelain with 2% (W / W) mass of velvet antler, and heat at 37°C Enzymolysis at high temperature for 0.5 to 24 hours; after the enzymolysis, adjust the pH to 8 with 2M NaOH, then add chymotrypsin with 1% antler mass, and enzymolysis at 50°C for 4 hours. Raise to 95°C and hold for 20 minutes.

[0057] The enzymatic solution was cooled to room temperature, centrifuged at a speed of 20000g for 10 minutes, and the supernatant was collected. The peptide concentration of the supernatant was determined by NanodropOnec, diluted with water to a peptide concentration of 30 mg / mL for use. The swollen Sephadex G-25 filler was packed into a gel column with a diameter of 10 cm and a ratio of column height to diameter of 10:1. Load the enzymolysis solution on the gel column, use deionized water as the eluent, and carry out e...

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Abstract

The invention relates to a cornu cervi glycopeptide and a preparation method and application. The cornu cervi glycopeptide prepared by the method can improve the memory reproduction ability of mice with reproduction memory disorders, has potential anti-Alzheimer's disease activity, can be applied to the prevention and treatment of cognitive disorders such as the Alzheimer's disease and is promising in application prospect.

Description

technical field [0001] The invention relates to velvet glycopeptide, in particular to a preparation method and application of velvet glycopeptide. Background technique [0002] Antler is the unossified and densely hairy young horns of the sika deer or red deer, an animal of the chordate phylum Mammalia deer family. Antler has been used as medicine for more than 2,000 years. "Compendium of Materia Medica" records that velvet "regenerates essence and nourishes marrow, nourishes blood and nourishes yang, strengthens muscles and bones, and cures all deafness, deafness, dark eyes, dizziness and dysentery". In recent years, studies have found that deer antler has various biological functions such as promoting nerve growth, immune regulation, anti-inflammation, anti-oxidation, and anti-aging. Antler contains proteins, glycosaminoglycans, phospholipids, fatty acids, hormone-like substances, nucleic acids, polyamines and various inorganic substances, among which antler protein is th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14A61K38/14A61P25/00A61P25/28A61P25/16
CPCC12P21/06C07K1/14A61P25/00A61P25/28A61P25/16A61K38/00
Inventor 靳艳叶明亮晏嘉泽
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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