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Perfluorocarbon oxygen-carrying microsphere and preparation method and application thereof

A perfluorocarbon and microsphere technology, which is applied in the field of nanomaterials and biomedical materials, can solve the problems of tissue damage and short oxygen release time, and achieve good biocompatibility, good affinity, and excellent pH sensitivity sexual effect

Inactive Publication Date: 2019-11-12
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the time for PFCs to release oxygen is very short, and excessive oxygen concentration will cause damage to the tissue, so it needs to be packaged in microspheres for slow release

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0035]

[0036] The preparation method of perfluorocarbon oxygen-carrying microspheres of the present invention comprises the following steps:

[0037] (1), preparation of PFCs aseptic emulsion: add egg yolk lecithin into Tyrode solution (Tyrode solution), use ultrasonic emulsification machine to emulsify 4-6 times at 25-30 ℃, each duration is 5- 10s, with an interval of 30-60s, to obtain the basic emulsion; add a perfluorocarbon stock solution with a volume fraction ≥ 97% in the basic emulsion, and use a ultrasonic emulsification machine to emulsify 4-7 times, and the emulsification time is 5-10s each time. After an interval of 30-60s, the original perfluorocarbon emulsion was obtained and set aside; the original perfluorocarbon emulsion was placed in a hyperbaric oxygen chamber to fully absorb oxygen to obtain a perfluorocarbon emulsion; The fluorocarbon emulsion is sterilized by filtration to obtain a perfluorocarbon aseptic emulsion for subsequent use;

[0038] (2), the...

Embodiment 1

[0055] The preparation method of the perfluorotributylamine oxygen-carrying microspheres of the present embodiment comprises the following steps:

[0056] (1), egg yolk lecithin was added to the Tyrode solution, and the mass percentage of egg yolk lecithin was 17%. At 25°C, it was emulsified 6 times using a ultrasonic emulsifier, with a power of 450W, and the duration of each time was 10s. 30s, get the basic emulsion;

[0057] (2), add the perfluorotributylamine stock solution with a volume fraction of 97% in the basic emulsion, the volume ratio of the basic emulsion and the perfluorotributylamine stock solution is 1:1, use a ultrasonic emulsifier to emulsify 7 times according to the above steps, The power is 450W, the time of each emulsification is 10s, and the interval between each emulsification is 60s, and the original emulsion of perfluorotributylamine is obtained, which is ready for use;

[0058] (3), put the original emulsion of perfluorotributylamine into the hyperbar...

Embodiment 2

[0065] The preparation method of perfluorocyclopropylamine and perfluorooctyl bromide oxygen-carrying microspheres of the present embodiment comprises the following steps:

[0066] (1) Add egg yolk lecithin to the Tyrode solution, the mass percentage of egg yolk lecithin is 20%, and emulsify 4 times at 25°C using a ultrasonic emulsifier, with a power of 350W, and the duration of each time is 5s. 30s, get the basic emulsion;

[0067] (2), add the volume fraction of 98% perfluorocyclopropylamine and perfluorooctyl bromide stock solution in the basic emulsion, the volume ratio of the basic emulsion and perfluorocyclopropylamine and perfluorooctyl bromide stock solution is 1.4:1, use The ultrasonic emulsification machine emulsified 4 times according to the above steps, the power was 350W, and the emulsification time was 5s each time, and the interval between each emulsification was 30s, to obtain the original emulsion of perfluorocyclopropylamine and perfluorooctyl bromide, and se...

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Abstract

The present invention provides a perfluorocarbon oxygen-carrying microsphere and a preparation method and an application thereof. The preparation method comprises the following steps: preparing perfluorocarbon into an emulsion, oxygenating and disinfecting the perfluorocarbon emulsion which is soluble in water and can carry oxygen, wrapping the emulsion in carboxymethyl chitosan to form microspheres, and connecting folic acid on surfaces of the microspheres to realize a targeting function. The perfluorocarbon oxygen-carrying microsphere is applied in treatments of tumors and alleviation of hypoxia. The perfluorocarbon is used as an oxygen-carrying material and wrapped in the carboxymethyl chitosan, and then the folic acid molecules are grafted to form the microspheres with a core-shell structure. The microsphere can realize a slow release effect of oxygen, besides, the shell layer material has good biocompatibility and targeting property, so that the microsphere can relieve an oxygen deficiency problem of a tumor part, realizes a function of slowly releasing oxygen at an oxygen deficiency part, and achieves a purpose of treating tumors. The perfluorocarbon oxygen-carrying microsphere is simple in the preparation method and convenient to operate, and the prepared oxygen-carrying microsphere can be stored for a long time.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biomedical materials, and in particular relates to a perfluorocarbon oxygen-carrying microsphere and its preparation method and application. Background technique [0002] In recent years, with the continuous improvement of living standards, people pay more and more attention to life safety. A series of diseases such as tumors and cancers seriously threaten people's health. Hypoxia is a common pathophysiological feature of most tumors, which is due to the imbalance of oxygen supply and oxygen consumption in cancer cells, resulting in abnormal new blood vessels and poor blood flow. For example, the effect of radiotherapy is greatly reduced. At present, researchers have found that tumor oxygen content can be promoted by different methods, such as by improving intratumoral blood flow, generating oxygen in situ within tumors, and delivering oxygen to tumors with oxygen carriers to treat tum...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K9/52A61K47/36A61K47/22A61K47/04A61K33/00A61P35/00
CPCA61K9/5036A61K9/501A61K9/5015A61K33/00A61P35/00
Inventor 袁伟忠周子璇
Owner TONGJI UNIV
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