Perfluorocarbon oxygen-carrying microsphere and preparation method and application thereof

A perfluorocarbon and microsphere technology, which is applied in the field of nanomaterials and biomedical materials, can solve the problems of tissue damage and short oxygen release time, and achieve good biocompatibility, good affinity, and excellent pH sensitivity sexual effect

Inactive Publication Date: 2019-11-12
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the time for PFCs to release oxygen is very short, and excessive oxygen concentratio

Method used

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Examples

Experimental program
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Example Embodiment

[0035]

[0036] The preparation method of perfluorocarbon oxygen-carrying microspheres of the present invention includes the following steps:

[0037] (1) Preparation of PFCs aseptic emulsion: add egg yolk lecithin to Tyrode solution (Tyrode solution), emulsify 4-6 times with a phacoemulsifier at 25-30℃, and the duration of each time is 5- 10s, with an interval of 30-60s, to obtain the basic emulsion; add the perfluorocarbon stock solution with a volume fraction of ≥97% into the basic emulsion, use a phacoemulsifier to emulsify 4-7 times, each emulsification time is 5-10s, each time The original perfluorocarbon emulsion is obtained at an interval of 30-60s for standby; the original perfluorocarbon emulsion is placed in the hyperbaric oxygen chamber to fully absorb oxygen to obtain the perfluorocarbon emulsion; the sterile filter is used to complete the oxygenation The fluorocarbon emulsion is filtered and sterilized to obtain a perfluorocarbon sterile emulsion for use;

[0038] (2...

Example Embodiment

[0054] Example 1:

[0055] The preparation method of perfluorotributylamine oxygen-carrying microspheres of this embodiment includes the following steps:

[0056] (1) Add egg yolk lecithin to Tyrode solution, the mass percentage of egg yolk lecithin is 17%, emulsify 6 times with a phacoemulsification machine at 25℃, the power is 450W, the duration of each time is 10s, the interval 30s, get the basic emulsion;

[0057] (2) Add the 97% volume fraction of perfluorotributylamine stock solution into the base emulsion, the volume ratio of the base emulsion and the perfluorotributylamine stock solution is 1:1, use a phacoemulsifier to emulsify 7 times according to the above steps, The power is 450W, the emulsification time is 10s each time, and the interval between each time is 60s to obtain the original perfluorotributylamine emulsion, which is ready for use;

[0058] (3) Put the original perfluorotributylamine emulsion in a hyperbaric oxygen chamber for 20 minutes to fully absorb oxygen t...

Example Embodiment

[0064] Example 2:

[0065] The preparation method of perfluorocyclopropylamine and perfluorooctyl bromide oxygen-carrying microspheres of this embodiment includes the following steps:

[0066] (1) Add egg yolk lecithin to Tyrode solution. The mass percentage of egg yolk lecithin is 20%. Use a phacoemulsifier to emulsify 4 times at 25°C. The power is 350W, and the duration of each time is 5s. 30s, get the basic emulsion;

[0067] (2) Add 98% volume fraction of perfluorocyclopropylamine and perfluorooctyl bromide stock solution into the base emulsion, the volume ratio of the base emulsion to the stock solution of perfluorocyclopropylamine and perfluorooctyl bromide is 1.4:1, use The phacoemulsifier emulsifies 4 times according to the above steps, the power is 350W, the time of each emulsification is 5s, and the interval is 30s between each time, to obtain the perfluorocyclopropylamine and perfluorooctyl bromide emulsions, which are ready for use;

[0068] (3) Put the perfluorocycloprop...

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Abstract

The present invention provides a perfluorocarbon oxygen-carrying microsphere and a preparation method and an application thereof. The preparation method comprises the following steps: preparing perfluorocarbon into an emulsion, oxygenating and disinfecting the perfluorocarbon emulsion which is soluble in water and can carry oxygen, wrapping the emulsion in carboxymethyl chitosan to form microspheres, and connecting folic acid on surfaces of the microspheres to realize a targeting function. The perfluorocarbon oxygen-carrying microsphere is applied in treatments of tumors and alleviation of hypoxia. The perfluorocarbon is used as an oxygen-carrying material and wrapped in the carboxymethyl chitosan, and then the folic acid molecules are grafted to form the microspheres with a core-shell structure. The microsphere can realize a slow release effect of oxygen, besides, the shell layer material has good biocompatibility and targeting property, so that the microsphere can relieve an oxygen deficiency problem of a tumor part, realizes a function of slowly releasing oxygen at an oxygen deficiency part, and achieves a purpose of treating tumors. The perfluorocarbon oxygen-carrying microsphere is simple in the preparation method and convenient to operate, and the prepared oxygen-carrying microsphere can be stored for a long time.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biomedical materials, and in particular relates to a perfluorocarbon oxygen-carrying microsphere and its preparation method and application. Background technique [0002] In recent years, with the continuous improvement of living standards, people pay more and more attention to life safety. A series of diseases such as tumors and cancers seriously threaten people's health. Hypoxia is a common pathophysiological feature of most tumors, which is due to the imbalance of oxygen supply and oxygen consumption in cancer cells, resulting in abnormal new blood vessels and poor blood flow. For example, the effect of radiotherapy is greatly reduced. At present, researchers have found that tumor oxygen content can be promoted by different methods, such as by improving intratumoral blood flow, generating oxygen in situ within tumors, and delivering oxygen to tumors with oxygen carriers to treat tum...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K9/52A61K47/36A61K47/22A61K47/04A61K33/00A61P35/00
CPCA61K9/5036A61K9/501A61K9/5015A61K33/00A61P35/00
Inventor 袁伟忠周子璇
Owner TONGJI UNIV
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