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Meroterpenoid compound with liver protecting nourishing functions, and applications thereof

A technology of mixed-source terpenoids and compounds is applied in the field of mixed-source terpenoids and in the field of preparing liver-protecting medicines, which can solve the problems such as the liver-protecting and liver-protecting effects that have not been seen yet, and the lack of research on biological activities, and achieve good research and development. Prospect, high yield effect

Active Publication Date: 2019-11-12
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the biogenic pathway of 3,5-dimethylorsanic acid mixed-source terpene can be obtained from the fermentation product of P. In the three aspects of antibacterial, anti-inflammatory and anti-tumor, there are no reports about the liver-protecting effect of this kind of mixed-source terpene compounds

Method used

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  • Meroterpenoid compound with liver protecting nourishing functions, and applications thereof
  • Meroterpenoid compound with liver protecting nourishing functions, and applications thereof
  • Meroterpenoid compound with liver protecting nourishing functions, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation method of mixed source terpenoids comprises the steps:

[0033] (1) An endophytic fungus isolated from Edgeworthia chrysantha, a traditional Chinese medicine of the genus Daphneaceae. The pure strain of this fungus was identified as purple-green by comparison of nucleic acid sequences using NCBI-BlastN 2.0.6+ Mold Penicillium purpurogenum.

[0034] (2) Preparation of strain liquid seed solution: the formula of the culture solution is 200g of potatoes, 10g of yeast extract, 6g of peptone, 30g of glucose, KH 2 PO 4 2g, MgSO 4 0.5g, dilute to 1L, adjust pH to 6.8. Pack into 500mL Erlenmeyer flasks, fill each bottle with about 150mL, sterilize at 115°C, 68kPa for 30 minutes. After the bacteria were inoculated, they were cultured at 28°C and 155 rpm for 3 days. Then the culture solution was diluted 100 times and used as seed solution for later use.

[0035] (3) Strain solid fermentation: 150g of rice and 180mL of purified water per 1L triangular bottl...

experiment example 1

[0049] Experimental example 1: Evaluation of the protective effect of compounds on human liver cell injury induced by acetaminophen (APAP) in vitro

[0050] 1. The effect of compounds on the proliferation of HepG2 cells

[0051] The MTT method is used. Human liver cancer HepG2 cells (the cells better retain the characteristics of normal human liver cells and are commonly used in liver injury research) were seeded in 96-well cell culture plates, and after 24 hours of culture, different concentrations of test samples or compounds were added, and at the same time A solvent control group was set up, and 3 parallel wells were set up for each drug concentration. After 48 hours of drug action on the cells, discard the culture medium, add 100 μL of MTT (0.5 mg / mL) solution to each well, continue to cultivate for 4 hours, discard the MTT solution, add 150 μL of DMSO to each well, oscillate with a mixing oscillator, and test the microplate reader at 570 nm. The absorbance value is mea...

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PUM

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Abstract

The invention discloses a meroterpenoid compound with liver protecting nourishing functions. The meroterpenoid compound with liver protecting nourishing functions comprises structures represented by formula I and II. It is confirmed by experiments that the meroterpenoid compound represented by the formula I and II possesses obvious protective effects on liver cell damage caused by paracetamol, sothat the meroterpenoid compound represented by the formula I and II can be taken as a liver protecting nourishing medicine, and is promising in study development prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a mixed-source terpenoid compound and its use in the preparation of liver-protecting and liver-protecting medicines. Background technique [0002] The liver is an important organ in the body that mainly has metabolic functions, and has the functions of storing glycogen and synthesizing secretory proteins. At the same time, it is the largest digestive gland in the human digestive system. important physiological functions. However, due to factors such as medication, drinking, improper diet, and excessive fatigue, it is very easy to cause liver damage. Among them, liver damage caused by drugs is the most serious, which is one of the most common and serious adverse drug reactions, and has risen to the No. 1 cause of death in the world. Five, so far there is still a lack of simple, objective and specific diagnostic indicators and specific treatment methods. Once the liv...

Claims

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Application Information

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IPC IPC(8): C07D493/22C07D493/16A61K31/366A61P1/16
CPCC07D493/22C07D493/16A61P1/16
Inventor 林生孙华石建功夏桂阳武玉卓张丹王玲燕张景芳李梅张凡
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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