Molecular marker closely linked with male and female plants of spinach (Spinacia oleracea), and application thereof
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A molecular marker, spinach technology, applied in the field of molecular genetics and breeding, can solve the problems of low accuracy, poor stability, affecting the purification speed of spinach inbred lines and female lines and the process of variety breeding, etc. The effect of shortening breeding time and improving breeding efficiency
Active Publication Date: 2019-11-12
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Although many sex-related molecular markers have been developed at home and abroad, most of these markers are dominant m
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Embodiment 1
[0037] Example 1 Development of Molecular Markers Closely Linked to Spinach Sex Gene
[0038] 1. Re-sequencing of high multipliers for female and male plants respectively
[0039] The young leaves of 20 female spinach and 20 male spinach were taken, and DNA was extracted by CTAB method. The extracted DNA samples were paired-end resequencing by Baimaike Biotechnology Co., Ltd. using the Illumina Xten platform. The read length of the sequencing sequence was 150 bp and the sequencing depth was 10×.
[0040]2. Female and male plant-specific sequence analysis
[0041] Using bwa (v0.7.17-r1188) software, the sequencing data of 20 female spinach and 20 male spinach were aligned to the reference genome of spinach (http: / / www.spinachbase.org / cgi-bin / spinach / index.cgi). Based on the results of the sequence alignment, the male-specific sequences were screened (Chr4:58765000-58790000) ( figure 1 ). Since the Y chromosome has one more sequence than the X chromosome genome, we used t...
Embodiment 2
[0047] Example 2 SponR used in molecular marker-assisted selection breeding
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Abstract
The invention provides a molecular marker closely linked with male and female plants of spinach (Spinacia oleracea) as well as application thereof. The molecular marker closely linked with male and female plants of spinach contains nucleotide sequence, of which the polymorphism is T / A, at locus 58764574 on chromosome 4 of spinach genome. According to the invention, the molecular marker closely linked with spinach sex gene is developed on basis of a KASP technology; and the molecular marker has been validated in a plurality of spinach populations to be closely linked with spinach sex gene. Thus, the molecular marker closely linked with spinach sex gene can be used for accurately identifying male and female plants of spinach. Being used for performing sex detection on spinach, the specificKASP primer provided by the invention is capable of achieving high analysis throughput and high accuracy; so that, the specific KASP primer is suitable for detection of large number of samples. The co-dominant molecular marker provided by the invention has important practical significance for improving female lines and inbred lines of spinach, shortening breeding time and improving breeding efficiency.
Description
technical field [0001] The invention belongs to the field of molecular genetics and breeding, in particular to a molecular marker closely linked with the sex of spinach and its application. Background technique [0002] Spinach (Spinacia oleracea L.), also known as Persian grass, red root vegetable, belongs to the genus Spinach of the family Ricaceae, and is a biennial herb with green leaves as the main product organ. Spinach is rich in plant fiber, protein, vitamins (carotene, vitamins B1, B2, B3, E, K, C, etc.), minerals (calcium, phosphorus, iron, potassium, etc.), folic acid and other nutrients. In addition, spinach has the characteristics of strong cold resistance, wide adaptability, short production cycle, high multiple cropping index, and high output value, so it is very popular among Chinese producers and consumers. According to the 2016 FAO statistics (http: / / www.fao.org / faostat / zh / ?#data / QC), my country's spinach output is about 24.47 million tons, accounting for ...
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IPC IPC(8): C12Q1/6895C12Q1/6879C12N15/11
CPCC12Q1/6895C12Q1/6879C12Q2600/156
Inventor 刘志远钱伟徐兆生折红兵李国亮张合龙王晓武武剑
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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