Molecular marker closely linked with male and female plants of spinach (Spinacia oleracea), and application thereof

Abstract

The invention provides a molecular marker closely linked with male and female plants of spinach (Spinacia oleracea) as well as application thereof. The molecular marker closely linked with male and female plants of spinach contains nucleotide sequence, of which the polymorphism is T / A, at locus 58764574 on chromosome 4 of spinach genome. According to the invention, the molecular marker closely linked with spinach sex gene is developed on basis of a KASP technology; and the molecular marker has been validated in a plurality of spinach populations to be closely linked with spinach sex gene. Thus, the molecular marker closely linked with spinach sex gene can be used for accurately identifying male and female plants of spinach. Being used for performing sex detection on spinach, the specificKASP primer provided by the invention is capable of achieving high analysis throughput and high accuracy; so that, the specific KASP primer is suitable for detection of large number of samples. The co-dominant molecular marker provided by the invention has important practical significance for improving female lines and inbred lines of spinach, shortening breeding time and improving breeding efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetics and breeding, in particular to a molecular marker closely linked with the sex of spinach and its application. Background technique [0002] Spinach (Spinacia oleracea L.), also known as Persian grass, red root vegetable, belongs to the genus Spinach of the family Ricaceae, and is a biennial herb with green leaves as the main product organ. Spinach is rich in plant fiber, protein, vitamins (carotene, vitamins B1, B2, B3, E, K, C, etc.), minerals (calcium, phosphorus, iron, potassium, etc.), folic acid and other nutrients. In addition, spinach has the characteristics of strong cold resistance, wide adaptability, short production cycle, high multiple cropping index, and high output value, so it is very popular among Chinese producers and consumers. According to the 2016 FAO statistics (http: / / www.fao.org / faostat / zh / ?#data / QC), my country's spinach output is about 24.47 million tons, accounting for ...

AI Technical Summary

Problems solved by technology

Although many sex-related molecular markers have been developed at home and abroad, most of these markers are dominant markers with low accuracy and poor stability. Purification speed and variety selection process of inbred and female lines

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