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Dried blood spot carrier material for desorbing electrospray ionization mass spectrum, and application thereof

A technology for desorbing electrospray and carrier materials, applied in the field of blood detection and metabolomics analysis, which can solve the problems of shedding, impurity signals of carrier materials, poor adhesion of blood spots, etc., and achieve the effect of improving strength

Active Publication Date: 2019-11-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest technical difficulty in combining DBSS with DESI-MS is the carrier material used for blood samples
In existing reports, blood samples are mainly spotted on cellulose-based materials, such as filter paper and blood sampling cards, but such materials have certain adsorption capacity for compounds, which affects the desorption effect of compounds in DESI-MS analysis, and will bring Impurity signals from the carrier material itself
Although the use of non-cellulose-based materials can avoid the disadvantages of cellulose-based materials to a certain extent, such as using glass slides as carrier materials, unfortunately, blood spots have poor adhesion to such materials and are easy to analyze in DESI-MS. collection failure

Method used

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  • Dried blood spot carrier material for desorbing electrospray ionization mass spectrum, and application thereof
  • Dried blood spot carrier material for desorbing electrospray ionization mass spectrum, and application thereof
  • Dried blood spot carrier material for desorbing electrospray ionization mass spectrum, and application thereof

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Effect test

Embodiment 1

[0043] A kind of dry blood spot carrier material that is used for desorption electrospray ionization mass spectrometry, preparation material comprises blank glass slide (76mm * 26mm) and PARAFILM; Preparation process is as follows:

[0044] Use a custom-made flat-bottomed cylinder to emboss each sampling slot in PAFAFILM as a circular three-dimensional groove, and the diameter of each sampling slot is 4-6 mm. In this implementation, a flat-bottomed cylinder with a diameter of 4 mm is used to imprint each sampling slot. The diameter of each sampling slot is 4mm, which can not only ensure that 5 μL of blood samples can be fixed in the carrier, but also enable more sample slots to be set on the same carrier material. The depth of each spotting groove is 1-2mm. In this implementation, a single-layer PARAFILM is used, and the embossing depth is about 1mm, which can ensure that the 5μL blood sample can be fixed in the carrier, and can also avoid the depth being too deep or too shallo...

Embodiment 2

[0046] A phospholipidomics analysis of the prepared dried blood spots based on dry blood spot sample preparation combined with desorption electrospray ionization mass spectrometer, comprising the following steps:

[0047] 1. Establishment of a mouse model of Schistosoma japonicum infection

[0048] Specific Pathogens Free (SPF) BALB / c female mice, 6–8 weeks old, with an average weight of 18±2g, were divided into normal control group (0dpi) and Schistosoma japonicum infection 42-day group (42dpi), each group 5 mice. The normal control group was not treated, and the mice in the infection group were infected according to the conventional method: take the snails positive for Schistosoma japonicum and place them in dechlorinated water, release the cercariae for 2 hours under the conditions of no wind, no vibration, and light at 25°C to 28°C, and inoculate them with The cercariae were collected by dipping the ring into the water surface onto a coverslip. After the mice were anesthet...

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Abstract

The invention discloses a dried blood spot carrier material for desorbing an electrospray ionization mass spectrum, and an application thereof in phospholipidomics; the dried blood spot carrier material is composed of a glass sheet and PARAFILM fixed on the glass sheet, and a sample application slot is formed in the PARAFILM. Since the PARAFILM is made of a non-cellulose base material, and the adsorption on compounds is weaker than that of a cellulose base material, so that the compounds can be desorbed more efficiently; in addition, the constituents of PARAFILM are very difficult to be ionized under the action of electrospray and are unlikely to bring impurity signals to the carrier material itself in mass spectrometry; and the sample application slot can prevent a blood sample from spreading on the one hand to improve the strength of the compounds, and can fix the blood spot on the other hand. By adopting the dried blood spot carrier material disclosed by the invention, the technicaldefects of the non-cellulose base material for DBSS in the DESI-MS analysis are solved, and it is possible to expand the research field of DBSS DESI-MS.

Description

technical field [0001] The invention relates to the technical field of blood detection and metabolomics analysis, more specifically, to a dry blood spot carrier material for desorption electrospray ionization mass spectrometry and its application in phospholipidomics. Background technique [0002] Phospholipids are the main components of all cell membranes, and they play an important role in the regulation of cell functions. The analysis of phospholipids will help us gain an in-depth understanding of the physiological functions of phospholipids in organisms and their connection with disease. Therefore, it is necessary to establish a set of efficient and high-throughput analytical detection methods that focus on the analysis of phospholipid compounds. [0003] With the development of mass spectrometry, in 2004, the Cooks team at Purdue University developed a direct, high-throughput analysis of samples, desorption electrospray ionization (DESI), that is, whether solid or liqui...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N27/64
CPCG01N1/28G01N27/64
Inventor 周海云陈剑松胡玥吕志跃
Owner SUN YAT SEN UNIV
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