Construction method and detection method of hplc fingerprint of Qianbo Biyan tablet

A technology of Qianbai Biyan Tablets and fingerprints, which is applied in the field of analysis and detection of traditional Chinese medicine, can solve problems such as difficulty in fully reflecting the overall quality characteristics of the product, inability to control product quality as a whole, complex chemical components, etc. Method optimization, the effect of a large number of chromatographic peaks

Active Publication Date: 2022-03-29
GUANGZHOU BAIYUNSHAN QIXING PHARMA
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

In this standard, there is only the qualitative identification of Senecio, Semen Cassiae, Ephedra, and Notopterygium by thin-layer chromatography. The content of hyperin, the main component of Senecio, is determined by HPLC. Qianbai Biyan Tablet is a traditional Chinese medicine compound. The chemical composition contained in the product is very complicated, and the standard is difficult to fully reflect the overall quality characteristics of the product, and cannot control the quality of the product as a whole.

Method used

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  • Construction method and detection method of hplc fingerprint of Qianbo Biyan tablet
  • Construction method and detection method of hplc fingerprint of Qianbo Biyan tablet
  • Construction method and detection method of hplc fingerprint of Qianbo Biyan tablet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Construction of Example 1 Qianbai Biyan Tablet HPLC Fingerprint

[0073] 1. Instrument: Agilent 1260 high performance liquid chromatograph, ML204 / 02 analytical balance.

[0074] 2. Reagents: Liquid chromatography analytical reagents are chromatographically pure, other reagents are analytically pure, and water is ultrapure water. Qianbai Biyan Tablets are provided by Guangzhou Baiyunshan Qixing Pharmaceutical Co., Ltd.

[0075] 3. Methods and results

[0076] 3.1 Mixed reference substance solution: Accurately weigh hyperoside, p-hydroxycinnamic acid, notopterin alcohol, chrysophanol, isoimperatorin, chlorogenic acid, imperatorin, abiflavonoids, emodin methyl ether, Emodin, osthole, caffeic acid, kaempferol reference substance amount, made by adding methanol. Each 1ml solution contains 200μg hyperoside, 100μg p-hydroxycinnamic acid, 60μg notopteryl alcohol, 100μg chrysophanol, 60μg isoimperiana A mixed reference solution of chlorogenic acid, 200 μg chlorogenic acid, 40 ...

Embodiment 2

[0154] The present embodiment provides a kind of detection method of Qianbai Biyan Tablets, the steps are as follows:

[0155] Preparation of the sample to be tested: Take Qianbai Biyan Tablets to be tested (batch numbers: 18001, 18020, 19007, 19008, 19009, provided by Guangzhou Baiyunshan Qixing Pharmaceutical Co., Ltd.), remove the coating from the sugar-coated tablet, grind finely, and accurately Weigh 1.6g, accurately add 25ml methanol, weigh the weight, extract by ultrasonic (power 250W, frequency 80kHz) for 60min, let cool, then weigh again, use the above methanol solvent to make up the lost weight, shake well, and use 0.45μm micrometer Pore ​​membrane filtration, take the continued filtrate, that is.

[0156] Chromatographic conditions: refer to "chromatographic conditions" under item 3.3 of Example 1.

[0157] Precisely draw 10 μl of the sample solution to be tested, inject it into the ultra-high performance liquid chromatograph, and inject the sample according to the...

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Abstract

The invention relates to a method for constructing the HPLC fingerprint of Qianbai Biyan Tablets, comprising the following steps: preparing a mixed reference substance solution: adding hyperin, p-hydroxycinnamic acid, notopterygium alcohol, chrysophanol, and isoprome The reference substances of carotene, chlorogenic acid, imperatorin, abiflavonoids, emodin methyl ether, emodin, osthole, caffeic acid and kaempferol were mixed reference substance solution; the preparation of the test solution : add Qianbai rhinitis tablet sample in the methanol of 25%-100% by volume fraction to extract, filter the extract and get the subsequent filtrate, which is the test solution; mix the reference substance solution with the test The product solution was respectively injected into a high-performance liquid chromatograph for measurement to obtain the fingerprint. The constructed fingerprint can fully reflect the quality information of Qianbai Biyan Tablets, and has the advantages of quickness, stability, high precision, and strong reproducibility, and can be used for the quality control of Qianbai Biyan Tablets.

Description

technical field [0001] The invention relates to the technical field of analysis and detection of traditional Chinese medicines, in particular to a construction method and a detection method of the HPLC fingerprint of Qianbai Biyan Tablets. Background technique [0002] Traditional Chinese medicine has always been the focus and difficulty of quality evaluation because of its complex components and chemical composition diversity and complexity. At present, fingerprints are internationally recognized as one of the most suitable means for controlling the quality of traditional Chinese medicines or natural medicines. From the perspective of the material basis of traditional Chinese medicines, fingerprints of traditional Chinese medicines can systematically, holistically and exclusively characterize traditional Chinese medicines by using modern analysis and detection technologies. The common and inherent characteristics of all. The "National Drug Standards and Technical Specifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N2030/047
Inventor 梁一彪
Owner GUANGZHOU BAIYUNSHAN QIXING PHARMA
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