Construction method of HPLC fingerprint and detection method of Qian Bai Bi Yan Pian

A technology of Qianbo Biyan tablet and fingerprint spectrum, applied in the field of traditional Chinese medicine analysis and detection, can solve the problems of complex chemical composition, inability to control product quality as a whole, difficult to fully reflect the overall quality characteristics of products, etc. The chromatogram baseline is stable and the chromatographic peak separation is good.

Active Publication Date: 2019-11-12
GUANGZHOU BAIYUNSHAN QIXING PHARMA
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AI Technical Summary

Problems solved by technology

In this standard, there is only the qualitative identification of Senecio, Semen Cassiae, Ephedra, and Notopterygium by thin-layer chromatography. The content of hyperin, the main component of Senecio, is determined by HPLC. Qianbai Biyan Tablet is a traditional Chinese medicine compound. The chemical composition contained in the product is very complicated, and the standard is difficult to fully reflect the overall quality characteristics of the product, and cannot control the quality of the product as a whole.

Method used

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  • Construction method of HPLC fingerprint and detection method of Qian Bai Bi Yan Pian
  • Construction method of HPLC fingerprint and detection method of Qian Bai Bi Yan Pian
  • Construction method of HPLC fingerprint and detection method of Qian Bai Bi Yan Pian

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Experimental program
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Effect test

Embodiment 1

[0072] Construction of Example 1 Qianbai Biyan Tablet HPLC Fingerprint

[0073] 1. Instrument: Agilent 1260 high performance liquid chromatograph, ML204 / 02 analytical balance.

[0074] 2. Reagents: Liquid chromatography analytical reagents are chromatographically pure, other reagents are analytically pure, and water is ultrapure water. Qianbai Biyan Tablets are provided by Guangzhou Baiyunshan Qixing Pharmaceutical Co., Ltd.

[0075] 3. Methods and results

[0076] 3.1 Mixed reference substance solution: Accurately weigh hyperoside, p-hydroxycinnamic acid, notopterin alcohol, chrysophanol, isoimperatorin, chlorogenic acid, imperatorin, abiflavonoids, emodin methyl ether, Emodin, osthole, caffeic acid, kaempferol reference substance amount, made by adding methanol. Each 1ml solution contains 200μg hyperoside, 100μg p-hydroxycinnamic acid, 60μg notopteryl alcohol, 100μg chrysophanol, 60μg isoimperiana A mixed reference solution of chlorogenic acid, 200 μg chlorogenic acid, 40 ...

Embodiment 2

[0154] The present embodiment provides a kind of detection method of Qianbai Biyan Tablets, the steps are as follows:

[0155] Preparation of the sample to be tested: Take Qianbai Biyan Tablets to be tested (batch numbers: 18001, 18020, 19007, 19008, 19009, provided by Guangzhou Baiyunshan Qixing Pharmaceutical Co., Ltd.), remove the coating from the sugar-coated tablet, grind finely, and accurately Weigh 1.6g, accurately add 25ml methanol, weigh the weight, extract by ultrasonic (power 250W, frequency 80kHz) for 60min, let cool, then weigh again, use the above methanol solvent to make up the lost weight, shake well, and use 0.45μm micrometer Pore ​​membrane filtration, take the continued filtrate, that is.

[0156] Chromatographic conditions: refer to "chromatographic conditions" under item 3.3 of Example 1.

[0157] Precisely draw 10 μl of the sample solution to be tested, inject it into the ultra-high performance liquid chromatograph, and inject the sample according to the...

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Abstract

The invention relates to a construction method of an HPLC fingerprint of Qian Bai Bi Yan Pian. The method comprises the steps of: carrying out preparation of a mixed reference substance solution, wherein hyperoside, p-hydroxycinnamic acid, notopterol, chrysophanol, isoimperatorin, chlorogenic acid, imperatorin, amentoflavone, physcion, emodin, osthole, caffeic acid and kaempferol reference substances are added into a solvent to obtain the mixed reference substance solution; carrying out preparation of a test substance solution, wherein a sample of Qian Bai Bi Yan Pian is added into methanol with a volume fraction of 25%-100% for extraction, and subsequent filtrate is obtained after filtration of extract to obtain the test substance solution; and respectively injecting the mixed reference substance solution and the test substance solution into a high performance liquid chromatography, and carrying out measurement and determination to obtain the fingerprint. The constructed fingerprint can comprehensively reflect quality information of Qian Bai Bi Yan Pian, has the advantages of fastness, stability, high precision, high reproducibility and the like, and can be used for quality control of Qian Bai Bi Yan Pian.

Description

technical field [0001] The invention relates to the technical field of analysis and detection of traditional Chinese medicines, in particular to a construction method and a detection method of the HPLC fingerprint of Qianbai Biyan Tablets. Background technique [0002] Traditional Chinese medicine has always been the focus and difficulty of quality evaluation because of its complex components and chemical composition diversity and complexity. At present, fingerprints are internationally recognized as one of the most suitable means for controlling the quality of traditional Chinese medicines or natural medicines. From the perspective of the material basis of traditional Chinese medicines, fingerprints of traditional Chinese medicines can systematically, holistically and exclusively characterize traditional Chinese medicines by using modern analysis and detection technologies. The common and inherent characteristics of all. The "National Drug Standards and Technical Specifica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N2030/047
Inventor 梁一彪张春波苏碧茹宁娜彭丹婷
Owner GUANGZHOU BAIYUNSHAN QIXING PHARMA
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