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Dendrobium nobile extract and preparation method thereof

A technology of Dendrobium chinensis and extract, applied in the field of Dendrobium chinensis extract and preparation, can solve the problems of harming the health of production technicians, high toxicity of chloroform, and high production cost, and achieves low cost, high active ingredient content, high production cost reduction effect

Active Publication Date: 2019-11-22
XIHUA UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the extraction and purification method of Dendrobium nobile alkaloids, there are some technical solutions (CN101653563 B; CN 101537128 B; CN 108175831 A; CN 101735231 B), but its defect is that a large amount of organic solvents are used, the production cost is high, and the energy consumption is large ; And some technical schemes have adopted chloroform treatment (CN 101537128 B; CN 104557956A; Zhao Fukun, Wu Qin, Lu Yuanfu, etc. Improvement of Dendrobium nobile total alkaloid extraction and content determination method. Journal of Zunyi Medical College, 2015,38 (5):532-535.; Cao Yunli, Pan Zihong, Zhao Xiaojun. Kinetic analysis of alkaloid extraction from Dendrobium nobile. Journal of Pingdingshan University, 2017,32(5):44-49.), the defect is that chloroform has relatively High toxicity, great risk of harming the body of production technicians

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of Dendrobium nobile extract, its preparation method is, prepare according to the steps of following order:

[0030] (1) Get 200g of Dendrobium nobile medicinal material, and break into granules;

[0031] (2) Take the granular raw material, soak it in a solvent for 8 hours, and then percolate, and collect the percolating fluid with a volume 30 times the weight of the raw material;

[0032] The solvent is an aqueous solution of hydrochloric acid at pH 2 containing a mixture of 3% glycine and ammonium acetate, and the molar ratio of the two in the mixture of glycine and ammonium acetate is 1:1.

[0033] (3) Take the percolation solution and use lime milk to adjust the pH to 6.5, put it on the D101 macroporous resin column, wash it with pure water of 3 times the column bed volume, discard the washing solution, and then use 4.5 times the column bed volume of pH 2 hydrochloric acid Aqueous solution elution, collect eluent A;

[0034] (4) Take the eluent A, adjust th...

Embodiment 2

[0040] A kind of Dendrobium nobile extract, its preparation method is, prepare according to the steps of following order:

[0041] (1) Get 200g of Dendrobium nobile medicinal material, and break into granules;

[0042] (2) Take the granular raw material, soak it in a solvent for 12 hours, and then percolate, and collect the percolation solution with a volume 50 times the weight of the raw material;

[0043] The solvent is an aqueous solution of hydrochloric acid with a pH of 1.5 containing 4% of a mixture of glycine and ammonium acetate, and the molar ratio of the two in the mixture of glycine and ammonium acetate is 1:1.5.

[0044] (3) Take the percolation solution and adjust the pH to 7 with milk of lime, put it on a D101 macroporous resin column, wash it with pure water of 3 times the volume of the column bed, discard the washing solution, and then use hydrochloric acid of pH 1.5 with 6 times the volume of the column bed Aqueous solution elution, collect eluent A;

[0045...

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Abstract

The invention provides a dendrobium nobile extract and a preparation method thereof, and the preparation method comprises the following steps: crushing dry dendrobium nobile stems as a raw material, soaking the material, and percolating the material to obtain a percolate; adjusting a pH value by using lime milk, passing through a macroporous resin column, eluting the material, adjusting the pH value of an eluent to be neutral, and concentrating the material under reduced pressure to obtain a clear paste; and uniformly stirring the clear paste with silica gel, carrying out silica gel column distribution chromatography to obtain the eluent, recovering the solution, recrystallizing the material, and drying and crushing the material to obtain the dendrobium nobile extract. Wherein the contentof total alkaloids contained in the dendrobium nobile extract is greater than 50%. The method has the advantages of reducing the use of organic solvents, reducing the risk of harm of toxic organic solvents to the bodies of production technicians, and reducing the production cost and energy consumption.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine extracts, and in particular relates to an extract of Dendrobium nobile and a preparation method thereof. Background technique [0002] The medicinal material of Dendrobium nobile Lindl is the dried stem of Dendrobium nobile Lindl, which has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. Dendrobium nobile contains a variety of alkaloid components such as dendrobium. Modern research shows that the total alkaloids of Dendrobium nobile have anti-diabetic, anti-hyperlipidemia, anti-senile dementia and other activities (Huang Qi. The effect of total alkaloids of Dendrobium nobile on diabetic rats The protective effect of the model. Zunyi Medical College, 2009.; Chen Jing. The effect of total alkaloids of Dendrobium nobile on ApoE - / - Effects of hyperlipidemia and vascular atherosclerosis in mice. Zunyi Medical College, 2016. Jiang Li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984
CPCA61K36/8984A61K2236/15A61K2236/333A61K2236/53A61K2236/55
Inventor 杨文宇钟爱琳王萌肖阳詹茂玲陈祥贵李明元唐显福王岚
Owner XIHUA UNIV
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