A kind of method for extracting coumarin from rough fern

A crude extraction technology of Pteris fern, applied in the direction of organic chemistry, etc., can solve the problems of difficult application in the field of medicine, low purity, etc.

Active Publication Date: 2022-07-22
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of the existing coumarin extraction method is low, and it is difficult to effectively apply it in the field of medicine

Method used

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  • A kind of method for extracting coumarin from rough fern
  • A kind of method for extracting coumarin from rough fern
  • A kind of method for extracting coumarin from rough fern

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) pulverize the roots (25kg) of dry rough fern, soaked in 125L of 95% ethanol for 6 hours, extract by reflux at 120 degrees Celsius, repeatedly extract 4 times, 1 hour each time, the extract is recovered under reduced pressure, and concentrated to obtain rough phoenix Coarse root extract of fern.

[0022] (2) Disperse the crude extract of Pteridophos chinensis in water, use 14-30 mesh polyamide resin column chromatography to elute, and carry out gradient elution with water and different concentrations of ethanol (30%, 60%, 95%) respectively , and the eluates of four parts were recovered under reduced pressure.

[0023] (3) 30% of the eluate extract was separated by medium pressure preparative liquid chromatography using the conditions that the volume solubility was 10%-80% methanol gradient elution to obtain 50 components, wherein the chromatographic column packing was ODS .

[0024] (4) 50 components prepared by medium pressure were recovered under reduced pressure...

Embodiment 2

[0027] (1) pulverize the roots (25kg) of dry rough fern, soaked in 125L of 95% ethanol for 6 hours, extract by reflux at 120 degrees Celsius, repeatedly extract 4 times, 1 hour each time, the extract is recovered under reduced pressure, and concentrated to obtain rough phoenix Coarse root extract of fern.

[0028] (2) Disperse the crude extract of Pteridophos chinensis in water, use 14-30 mesh polyamide resin column chromatography to elute, and carry out gradient elution with water and different concentrations of ethanol (30%, 60%, 95%) respectively , and the eluates of four parts were recovered under reduced pressure.

[0029] (3) 30% of the eluate extract was separated by medium pressure preparative liquid chromatography using the conditions that the volume solubility was 10%-80% methanol gradient elution to obtain 50 components, wherein the chromatographic column packing was ODS .

[0030] (4) 50 components prepared by medium pressure were recovered under reduced pressure...

Embodiment 3

[0032] (1) pulverize the roots (25kg) of dry rough fern, soaked in 125L of 95% ethanol for 6 hours, extract by reflux at 120 degrees Celsius, repeatedly extract 4 times, 1 hour each time, the extract is recovered under reduced pressure, and concentrated to obtain rough phoenix Coarse root extract of fern.

[0033] (2) Disperse the crude extract of Pteridophos chinensis in water, use 14-30 mesh polyamide resin column chromatography to elute, and carry out gradient elution with water and different concentrations of ethanol (30%, 60%, 95%) respectively , and the eluates of four parts were recovered under reduced pressure.

[0034] (3) 30% of the eluate extract was separated by medium pressure preparative liquid chromatography using the conditions that the volume solubility was 10%-80% methanol gradient elution to obtain 50 components, wherein the chromatographic column packing was ODS .

[0035] (4) 50 components prepared by medium pressure were recovered under reduced pressure...

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Abstract

The invention relates to a method for extracting coumarin from Pteridophos chinensis, belonging to the field of natural product extraction. The invention completes the extraction of coumarin from Pteridopsis raspberry through four steps of crude extraction of the root of Pteridophos chinensis, separation by polyamide resin column chromatography, medium-pressure preparation of eluate, and detection and preparation by high performance liquid chromatography. The invention adopts HPLC equipped with a DAD detector to quickly lock these trace compounds from a chromatogram, and then directionally prepares trace compound monomers. The prepared coumarin has a high purity, and the content of the coumarin can reach more than 95% by high performance liquid chromatography.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and in particular relates to a method for extracting coumarin from Pteridophos chinensis. Background technique [0002] The rough fern belongs to the genus Pteridaceae, and the whole plant of the fern contains ergosterol, choline, tannins, glycosides, etc. The whole plant can be used as medicine, and has the functions of lowering blood pressure, deworming, and preventing cancer. Nephritis and lung diseases also have good curative effect, so it is called "longevity vegetable". Chinese medicine believes that fern is cold in nature and sweet in taste. "Therapeutic Materia Medica" claims that it can supplement the insufficiency of the five internal organs, and also treats qi obstruction, meridians, and bones. "Compendium of Materia Medica" states that bracken can remove violent heat, benefit small water, and treat diseases such as food and gas, intestinal wind and heat poisoning, and can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/16
CPCC07D311/16
Inventor 吴娇李春斌李杨尹瀚林石玉生
Owner DALIAN NATIONALITIES UNIVERSITY
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