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Polymorphic molecular marker for identifying Xinjiang color cotton varieties and application thereof

A technology of molecular markers and polymorphism, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of few reports on the construction of DNA fingerprints of colored cotton varieties in Xinjiang, and achieve the value of genetic effects Reliable, time-sensitive and sensitive results

Active Publication Date: 2019-12-03
SHIHEZI UNIVERSITY
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AI Technical Summary

Problems solved by technology

Research on the construction of cotton DNA fingerprints based on molecular markers has also made great progress, but there are few reports on the construction of DNA fingerprints of Xinjiang colored cotton varieties

Method used

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  • Polymorphic molecular marker for identifying Xinjiang color cotton varieties and application thereof
  • Polymorphic molecular marker for identifying Xinjiang color cotton varieties and application thereof
  • Polymorphic molecular marker for identifying Xinjiang color cotton varieties and application thereof

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Embodiment 1

[0064] 1. Materials

[0065] The methods used in the present embodiment are conventional methods known to those skilled in the art unless otherwise specified, and materials such as reagents used, if not otherwise specified, are commercially available products.

[0066] 2. Method

[0067] The present invention screens according to comprehensive characteristics such as polymorphism, stability, and identification ability, obtains 17 pairs of primers with excellent comprehensive characteristics, and uses 27 varieties of Xinjiang colored cotton as materials to construct DNA fingerprints of new colored cotton, and obtains available Molecular markers for identifying 27 varieties of colored cotton in Xinjiang. The molecular markers include characteristic primers of 11 Xinjiang colored cotton varieties and combination primers of 16 Xinjiang colored cotton varieties. And at least 10 pairs of core primers are used to completely distinguish 27 new colored cotton varieties, and the ten-d...

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Abstract

The invention relates to polymorphic molecular markers for identifying Xinjiang color cotton varieties and application thereof, and belongs to the technical field of molecular detection. Through 17 pairs of screened polymorphic identification primers, fingerprint construction and molecular identification of 27 varieties of Xinjiang color cotton are realized. The 17 pairs of primers not only have advantages of high polymorphism, good stability, strong timeliness, simple operation, strong sensitivity, etc., but also can accurately detect and lock target genes, and have more reliable genetic effect values. The different allelic variations reflected by the primers are directly related to phenotypes, and field phenotypic traits are closely connected with indoor molecular markers. Only 10 pairsof core primers at minimum are needed to identify 27 varieties. The polymorphic molecular markers are more practical.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a polymorphic molecular marker for identifying Xinjiang colored cotton varieties and its application. Background technique [0002] With the rapid development of the cotton industry, the cotton seed market has become more and more serious, and the quality of the seeds has been declining, which seriously hinders the healthy development of the cotton industry. Therefore, the cultivation and protection of high-quality cotton varieties has become a common issue discussed by breeding researchers at home and abroad. Traditional morphological identification methods have disadvantages such as long period, low cost, short validity period, easy to be affected by the environment, poor reliability, etc., and it is difficult to adapt to the needs of the development of the new situation. Molecular marker technology can fully adapt to the needs of the development of new...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11C12Q1/686
CPCC12Q1/6895C12Q1/686C12Q2600/156C12Q2600/13C12Q2565/125
Inventor 薛飞李婧慧金彦龙李淑琴孙杰
Owner SHIHEZI UNIVERSITY
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