A method for constructing an animal model of rheumatoid arthritis combined with interstitial lung disease

Abstract

The invention discloses a method for constructing an animal model of rheumatoid arthritis combined with interstitial lung disease, comprising the following steps: (1) mixing type II collagen and an equal volume of complete Freund's adjuvant to prepare an immune preparation; The immunization preparation was injected intradermally at multiple points at the base of the tail to complete the first immunization, and the immunization was boosted 21 d after the first immunization to construct CIA model mice; (2) CIA model mice were successfully constructed by indirect perfusion on the 4th day after the successful establishment of CIA model mice. The model mice were injected with bleomycin into the trachea. After 30 days, the animal model of rheumatoid arthritis combined with interstitial lung disease was established. The above method is convenient for large-scale operation and has strong reproducibility. The obtained animal model can better simulate the development of chronic progressive pulmonary fibrosis and the repair process of microenvironmental damage caused by autoimmune diseases, and can be used for subsequent pathological mechanisms and disease treatment. The research provides a good experimental platform for animal models.

Description

technical field [0001] The invention relates to the technical field of animal model construction, and more specifically, relates to a method for constructing an animal model of rheumatoid arthritis combined with interstitial lung disease. Background technique [0002] Symmetrical arthritis is the common clinical symptom of rheumatoid arthritis (RA), and the joints most commonly involved are the wrists, metacarpophalangeals, and proximal knuckles. In addition, the skin, eyes, lungs, and heart can also be involved. , nervous system, etc. are autoimmune diseases based on chronic arthritis. According to reports, 21% of RA patients have respiratory symptoms, 39% of patients have abnormal chest X-rays, and 14% have both. It is not uncommon to have lung changes. Rheumatoid arthritis is a common clinical disease, which invades the connective tissue and blood vessels of the whole body. Because the lung is rich in connective tissue and blood vessels, RA patients are often accompanied...

AI Technical Summary

Problems solved by technology

[0003] Animal disease models are mainly used in the research of experimental physiology, experimental pathology and experimental therapeutics. The development of human diseases is very complicated. Using humans as experimental objects to deeply explore the mechanism of disease occurrence will promote the development of medicine. Not only is experience limited in time and space, but many experiments are also morally and methodologically constrained

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