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Lactobacillus plantarum for efficiently lowering in-vivo cholesterol level and application of lactobacillus plantarum

A Lactobacillus plantarum, high cholesterol technology, applied in the field of microorganisms, can solve problems such as difficult recovery, and achieve the effects of improving liver damage, promoting excretion, and inhibiting absorption

Active Publication Date: 2019-12-27
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, blood lipid levels can be controlled within an ideal range through diet and drugs, but a series of complications, especially chronic complications, are difficult to recover and require long-term conditioning

Method used

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  • Lactobacillus plantarum for efficiently lowering in-vivo cholesterol level and application of lactobacillus plantarum
  • Lactobacillus plantarum for efficiently lowering in-vivo cholesterol level and application of lactobacillus plantarum
  • Lactobacillus plantarum for efficiently lowering in-vivo cholesterol level and application of lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Isolation and Identification of Plant Lactobacillus Lactobacillus plantarum H6

[0044] H6 and T19 strains were isolated from Nianmianzi and Tangzinian respectively in Heilongjiang area. After being activated twice, they were inoculated into MRS medium containing 100μg / mL cholesterol at 2% inoculum, and cultured anaerobically at 37°C for 24h. in subsequent experiments.

[0045] The H6 and T19 strains were identified as Lactobacillus plantarum by 16S rRNA identification.

Embodiment 2

[0046] Embodiment 2 animal feeding experiment

[0047] 32 male C57BL / 6 mice (5 weeks old, body weight 17-18 g) SPF grade were purchased from Shenyang Changsheng Biological Co., Ltd. (approval number SCXK 2015-0001 Liaoning, China), and the procedures involving mice were strictly carried out in accordance with international animal care standards , and were approved by the Jilin Agricultural University Animal Protection Institution and Use Committee.

[0048] Mice were adaptively cultured at room temperature (25±1° C.) under a cycle of 12 hours of light and 12 hours of darkness. After 1 week, the mice were divided into 4 groups with 8 mice in each group.

[0049] Each group was fed with:

[0050] (1) NC, normal diet LAD0011 (Nantong Trophy Feed Technology Co., Ltd.), as a control group;

[0051] (2) HC, high cholesterol diet TP06105 (Nantong Trophy Feed Technology Co., Ltd.): containing 1.25% cholesterol, 0.5% bile salt;

[0052] (3) HC+H6, high cholesterol diet + Lactobacil...

Embodiment 3

[0056] The influence of embodiment 3 Lactobacillus plantarum on blood fat, bile acid

[0057] Centrifuge the blood sample (3500g, 4°C) for 3min to separate the serum; use TC, TG, LDL-C, HDL-C test kits (Nanjing Jiancheng Bioengineering Institute) to test TC, TG, LDL-C, HDL-C respectively Detect; use the total bile acid test box (Nanjing Jiancheng Bioengineering Institute) to measure the total bile acid content.

[0058] Accurately weigh the mouse liver tissue, add 9 times the volume of normal saline according to (mass:volume = 1:9), homogenate in ice bath, centrifuge (2500g, 4°C) for 10min after homogenization, use TC, TG The test box (Nanjing Jiancheng Bioengineering Institute) was used to measure the content of TC and TG in the supernatant; the total bile acid content was measured using the total bile acid test box (Nanjing Jiancheng Bioengineering Institute).

[0059]Accurately weigh 250 mg of feces from mice in each group and add 9 times the volume of normal saline to hom...

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Abstract

The invention discloses lactobacillus plantarum H6 for efficiently lowering an in-vivo cholesterol level. The lactobacillus plantarum H6 is collected in General Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a collection number of CGMCC No. 18205. The lactobacillus plantarum H6 can be used for effectively improving alteration of intestinal florae and liver injury induced by high-cholesterol diets.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a plant lactobacillus and application thereof. Background technique [0002] A long-term high-cholesterol diet will not only lead to obesity, but also cause hyperlipidemia, causing a series of complications: such as damaging the intestinal flora, triggering inflammatory factors, leading to chronic intestinal diseases, and even inducing intestinal cancer in severe cases; Fat metabolism disorder, fatty liver, severe cases cause hepatitis, liver cirrhosis. [0003] During the treatment process, it is usually necessary to establish good living habits, strengthen exercise, diet control and drug control. However, blood lipid levels can be controlled within an ideal range through diet and drugs, but a series of complications, especially chronic complications, are difficult to recover and require long-term conditioning. [0004] Therefore, how to effectively impr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/06A61P1/16A61P1/00A23L33/135C12R1/25
CPCA61K35/747A61P3/06A61P1/16A61P1/00A23L33/135A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/3204A23V2200/3262
Inventor 任大勇曲天铭杨柳安彬王国超冯时荣王玉华闫薇周烁浛吕慧娟孙畅刘宏妍
Owner THANKCOME BIOLOGICAL SCI & TECH CO LTD
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