Plant regeneration rapid propagation technology using passion fruit leaves

A passion fruit and leaf technology, which is applied in the field of passion fruit leaf plant regeneration and rapid propagation technology, can solve the problems of long cycle, low growth cost, short growth cycle, etc.

Inactive Publication Date: 2020-01-03
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a passion fruit leaf plant regeneration and rapid propagation technology, which has the advantages of short growth period, not affected by the environment and weather, and l

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Passion fruit blade plant regeneration rapid propagation technology comprises the following steps:

[0029] 1) Selection of explants. Select purple passion fruit and 3-4cm long stem tips in sunny days with strong growth, good growth and no pests and diseases. The fruit is rinsed under tap water, and the surface of the fruit is scrubbed with soapy water. After cleaning, In the aseptic workbench, wipe the fruit epidermis with 75% alcohol repeatedly for 4-5 times for later use, cut off the old leaves that are not needed at the stem tip, rinse repeatedly with tap water, and then wash with soapy water. After cleaning, put them in the aseptic workbench. , first soak in 75% alcohol for 30s, then soak in 0.1% mercuric chloride solution for 10min, then rinse with sterile water for 4-5 times, then dry the surface with sterile filter paper, and set aside.

[0030] 2) Cut the aseptically treated fruit from the middle, select a large, plump seed and directly inoculate it into a ster...

Embodiment 2

[0034] Passion fruit blade plant regeneration rapid propagation technology comprises the following steps:

[0035] 1) Selection of explants. Select purple passion fruit and 3-4cm long stem tips in sunny days with strong growth, good growth and no pests and diseases. The fruit is rinsed under tap water, and the surface of the fruit is scrubbed with soapy water. After cleaning, In the aseptic workbench, wipe the fruit epidermis with 75% alcohol repeatedly for 4-5 times for later use, cut off the old leaves that are not needed at the stem tip, rinse repeatedly with tap water, and then wash with soapy water. After cleaning, put them in the aseptic workbench. , first soak in 75% alcohol for 30s, then soak in 0.1% mercuric chloride solution for 10min, then rinse with sterile water for 4-5 times, then dry the surface with sterile filter paper, and set aside.

[0036] 2) Cut the aseptically treated fruit from the middle, select a large, plump seed and inoculate it directly into a ster...

Embodiment 3

[0044] Passion fruit blade plant regeneration rapid propagation technology comprises the following steps:

[0045] 1) Selection of explants. Select purple passion fruit and 3-4cm long stem tips in sunny days with strong growth, good growth and no pests and diseases. The fruit is rinsed under tap water, and the surface of the fruit is scrubbed with soapy water. After cleaning, In the aseptic workbench, wipe the fruit epidermis with 75% alcohol repeatedly for 4-5 times for later use, cut off the old leaves that are not needed at the stem tip, rinse repeatedly with tap water, and then wash with soapy water. After cleaning, put them in the aseptic workbench. , first soak in 75% alcohol for 30s, then soak in 0.1% mercuric chloride solution for 10min, then rinse with sterile water for 4-5 times, then dry the surface with sterile filter paper, and set aside.

[0046] 2) Cut the aseptically treated fruit from the middle, select a large, plump seed and directly inoculate it into a ster...

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PUM

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Abstract

The invention relates to the technical field of passion fruit tissue culture and discloses a plant regeneration rapid propagation technology using passion fruit leaves. The plant regeneration rapid propagation technology includes the steps of firstly, selecting explants, to be more specific, selecting the fruits, which grow robustly and are good in growth vigor and free of diseases and pests, of purple-fruit passion fruit and twig stem tips 3-4cm in length, washing the fruits with tap water, and brushing the surfaces of the fruits with soapy water, wherein the surfaces of the fruits cannot bedamaged. The plant regeneration rapid propagation method using the cotyledons, true leaves and field step tip tender leaves of passion fruit seed sterile seedlings as the propagation materials is researched through experimental comparison, experiment results show that the cotyledons and true leaves of the seedlings can produce calluses through induction culture, especially the cotyledons are earlyin callus differentiation and fast in growth, callus induction rate can reach 100%, adventitious bud bundles can be directly differentiated during callus production, the true leaves are in the secondplace, and the experiments on the stem tip tender leaves fail.

Description

technical field [0001] The invention relates to the technical field of passion fruit tissue culture, in particular to a passion fruit leaf plant regeneration and rapid propagation technology. Background technique [0002] Passion fruit, also known as egg fruit, passion fruit, and pomegranate, belongs to the teneben plant of the genus Passiflora in the family Passiflora. It is a tropical and subtropical perennial plant. The fruit is rich in 17 kinds of amino acids, multivitamins, and Carotene and trace elements, high nutritional value, 5-16% soluble solids, 3.8-4.0% total acid, moderate sweet and sour, known as the "king of juice", its roots and leaves can be used as medicine, with anti-inflammatory , pain relief, blood circulation and physical strength and other effects. [0003] In recent years, with the improvement of people's understanding of passion fruit, there have been many studies on the comprehensive development and utilization of passion fruit and high-yield culti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨宝明黄玉玲李永平李迅东尹可锁
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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