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Application of dendramine and composition thereof in aspect of thyroid cancer resistance

A technology of dendrobium base and thyroid cancer, which is applied in the field of biomedicine and can solve the problems of thyroid cancer without dendrobium base and the like

Inactive Publication Date: 2020-01-10
南京乐扬医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the use of dendrobium and isocarpentol alone or in combination for the treatment of thyroid cancer

Method used

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  • Application of dendramine and composition thereof in aspect of thyroid cancer resistance
  • Application of dendramine and composition thereof in aspect of thyroid cancer resistance
  • Application of dendramine and composition thereof in aspect of thyroid cancer resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Proliferation inhibitory effect on human thyroid cancer TPC-1 cells

[0016] 1. Test materials

[0017] Human thyroid carcinoma TPC-1 cells were purchased from ATCC. Penicillin, MTT, and dimethyl sulfoxide were purchased from Sigma. The HPLC purity of Dendrobium amine base and isocyperol is ≥98%. Fetal bovine serum was purchased from Gibco, and RPMI1640 medium was purchased from Solarbio.

[0018] 2. Test method

[0019] 1. Cell culture and grouping

[0020] TPC-1 cells were cultured in RPMI1640 containing 10% fetal bovine serum, 100 U / ml penicillin, 100 μg / ml streptomycin based on 5% CO 2 , Cultured in a 37°C incubator, subcultured every 2-3 days, and the logarithmic phase cells were taken for subsequent experiments.

[0021] Grouped as follows:

[0022] Dendrobium low concentration group: cultured with RPMI1640 containing 10% fetal bovine serum, 100U / ml penicillin, 100μg / ml streptomycin, 20nM dendrobylamine based on 5% CO 2 , Cultivated in a 37°C in...

Embodiment 2

[0036] Example 2: Proliferation inhibitory effect on human thyroid cancer SW579 cells

[0037] 1. Test materials

[0038] Human thyroid carcinoma SW579 cells were purchased from ATCC. Penicillin, MTT, and dimethyl sulfoxide were purchased from Sigma. The HPLC purity of Dendrobium amine base and isocyperol is ≥98%. Fetal bovine serum was purchased from Gibco, and RPMI1640 medium was purchased from Solarbio.

[0039] 2. Test method

[0040] 1. Cell culture and grouping

[0041] SW579 cells were cultured in RPMI1640 containing 10% fetal bovine serum, 100 U / ml penicillin and 100 μg / ml streptomycin based on 5% CO 2 , Cultured in a 37°C incubator, subcultured every 2-3 days, and the logarithmic phase cells were taken for subsequent experiments.

[0042] Grouped as follows:

[0043]Dendrobium low concentration group: cultured with RPMI1640 containing 10% fetal bovine serum, 100U / ml penicillin, 100μg / ml streptomycin, 20nM dendrobylamine based on 5% CO 2 , Cultivated in a 37°C ...

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PUM

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Abstract

The invention discloses application of dendramine and a composition of the dendramine in the aspect of thyroid cancer resistance. Test results prove that the dendramine and the composition thereof disclosed by the invention are both capable of effectively inhibiting proliferation of human thyroid cancer cells; and moreover, being used in combination, the dendramine and isocyperol can synergistically take an effect. Thus, the dendramine and the composition thereof can be developed into anti-thyroid-cancer drugs.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the application of a dendrobium base and a composition thereof in anti-thyroid cancer. Background technique [0002] Although thyroid cancer accounts for only 5% of thyroid nodules and 1% of systemic tumors, it is the most common malignant tumor of the endocrine system. In 2000, the Tumor Registry Association reported that the global incidence of thyroid cancer was 1.2 / 100,000 males and 3.0 / 100,000 females, and it was on the rise year by year; a report in 2005 showed that the global incidence increased by 4% per year, leaping to the top of the list. The 8th most common tumor in women, with nearly 900 new cases per year in the UK and Wales; France 1978-2001, thyroid cancer became one of the tumors with the largest increase in incidence rate in women, and in 2007, Uhry et al., standardized according to the age of the world population It is concluded that the incidence rate of thyroid canc...

Claims

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Application Information

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IPC IPC(8): A61K31/407A61K31/045A61P35/00
CPCA61K31/045A61K31/407A61P35/00A61K2300/00
Inventor 周天北
Owner 南京乐扬医药科技有限公司