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New application of fibrinogen gamma chain in the field of tooth regeneration and its kit

A technology of fibrinogen and kits, which is applied in the direction of peptide/protein components, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of undisclosed application of fibrinogen γ chains, etc., and achieve significant tooth regeneration, The effect of improving efficiency

Active Publication Date: 2020-09-29
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fibrinogen gamma chain (FGG), as the main chain part of fibrinogen, does it also have the same biological function as fibrinogen? Fibrinogen gamma chain (FGG) is a secreted blood zymogen , its activation needs to be transformed into plasmin and angiostatin through proteolysis, respectively to reduce thrombus formation and inhibit angiogenesis. At present, the routine application of fibrinogen at home and abroad is for the detection of kidney disease, diabetes, nausea and tumor, craniocerebral injury, etc. On the one hand, for example, the patent with the publication number CN107505461A discloses a fibrinogen gamma chain as a marker for assessing the severity of traumatic brain injury. The current prior art does not disclose the application of the fibrinogen gamma chain in the dental field

Method used

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  • New application of fibrinogen gamma chain in the field of tooth regeneration and its kit
  • New application of fibrinogen gamma chain in the field of tooth regeneration and its kit
  • New application of fibrinogen gamma chain in the field of tooth regeneration and its kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 4

[0038] Example 4: Isolation, cultivation and passage of primary human dental pulp stem cells (hDPSCs)

[0039] 1. Reagent preparation, culturing primary dental pulp stem cells, preparing for the subsequent proof that FGG promotes odontogenic differentiation of dental pulp stem cells, and providing cell materials. The preparation process is as follows.

[0040](1) 20% FBS serum primary medium: Add 10mL penicillin-streptomycin solution and 100mL fetal bovine serum to 390mL L-DMEM medium, shake well, aliquot into 50mL centrifuge tubes, and store in a refrigerator at 4°C spare.

[0041] (2) Type Ⅰ collagen dissolving enzyme solution: add 100 mL L-DMEM medium to 300 mg type Ⅰ collagenase powder to make a concentration of 3 mg / mL type Ⅰ collagenase; add 100 mL L-DMEM medium to 400 mg dispase powder to prepare 4 mg / mL type Ⅰ collagenase; mix 3 mg / mL type Ⅰ collagenase and 4 mg / mL dispase at a ratio of 1:1, pass through a 0.22 μm filter membrane to filter and sterilize, and mix well ...

Embodiment 5

[0051] Example 5 qRT-PCR detection of the effect of FGG on the expression of RNA in odontogenic differentiation of dental pulp stem cells

[0052] figure 1 It is a graph of qRT-PCR detecting the effect of FGG on the odontogenic differentiation of dental pulp stem cells at the gene level. figure 1 In A, dentin sialophosphoprotein (dentinsialophosphoprotein, DSPP), B, dentin matrix protein-1 (dentin matrix protein-1, DMP1), and C, bone sialoprotein (Bone Sialoprotein, BSP), D is the gene detection result of Sp7 (Osterix, OSX). The test results showed that: FGG can significantly improve the odontogenic differentiation ability of dental pulp stem cells, and the gene expression of odontogenic markers DSPP, DMP1, BSP and OSX was significantly increased, suggesting that FGG can significantly promote the odontogenic differentiation ability.

[0053] The qRT-PCR detection process is as follows:

[0054] (1) Grouping: control group: cell culture medium induced by normal mineralizatio...

Embodiment 6

[0067] Example 6: Western blot detection of the effect of FGG on the expression of odontogenic differentiation proteins in dental pulp stem cells

[0068] figure 2 It is a Western blot to evaluate the effect of FGG on the odontogenic differentiation of dental pulp stem cells at the protein level. After 14 days of odontogenic differentiation of dental pulp stem cells, the odontogenic markers dentine sialophosphoprotein (DSPP), dentin matrix protein 1 (dental matrix protein-1, DMP1) BSP (Bone Sialoprotein, BSP), OSX (Sp7 / Osterix, OS X) expression. The test results showed that: FGG can significantly improve the odontogenic differentiation ability of dental pulp stem cells, and the expressions of odontogenic markers DSPP, DMP1, BSP and OSX were significantly increased, suggesting that FGG has the ability to significantly promote the odontogenic differentiation of dental pulp stem cells.

[0069] The Western blot detection process is as follows:

[0070] (1) Grouping: control ...

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Abstract

The invention discloses an application of a fibrinogen gamma chain as a dental pulp dentin regeneration reagent, and provides a kit for promoting dental pulp dentin regeneration. The kit comprises thefibrinogen gamma chain as a growth factor for promoting odontoblast differentiation of dental pulp stem cells, can significantly improve an efficiency of the dentin differentiation of the dental pulpstem cells, provides a new method for promoting dental pulp dentin regeneration, and has a significant tooth regeneration effect.

Description

technical field [0001] The invention belongs to the application field of biological reagents, and in particular relates to the application field of new uses of proteogen cells. Background technique [0002] Pulp and periapical infection is one of the most common clinical diseases in the oral cavity, and root canal therapy is a routine treatment for infected pulp in clinical practice. Despite its satisfactory clinical efficacy in terms of infection control and pain relief, traditional treatment modalities only seal the space of the root canal system without restoring original pulp tissue function and vitality. Without the blood, nerve and nutritional support provided by the pulp tissue, teeth after root canal treatment are more prone to various complications such as crown discoloration, root fracture and reinfection. Long-term studies have shown that the rate of tooth loss after root canal treatment is much higher than that of untreated teeth. Therefore, it is an urgent cli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/36A61P1/02
CPCA61K38/363A61P1/02
Inventor 田智慧邵龙泉
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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