Nano-particle and gel for promoting dentin regeneration as well as preparation method and application of nano-particle and gel

A particle and matrigel technology, applied in the field of biomedicine, can solve problems such as the probability of tooth fracture, unsatisfactory long-term curative effect, and increase

Active Publication Date: 2021-05-28
CAPITAL UNIVERSITY OF MEDICAL SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The apex of young permanent teeth is not yet fully developed, the root canal opening is open, and the root canal wall is thin. Root canal treatment cannot effectively prepare and fill the root canal, n

Method used

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  • Nano-particle and gel for promoting dentin regeneration as well as preparation method and application of nano-particle and gel
  • Nano-particle and gel for promoting dentin regeneration as well as preparation method and application of nano-particle and gel
  • Nano-particle and gel for promoting dentin regeneration as well as preparation method and application of nano-particle and gel

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preparation example Construction

[0028] The invention provides a preparation method of AZD2858-CD-chitosan nanoparticles for promoting dentin regeneration, comprising the following steps: the concentration of AZD2858 in the diluent is 50-150nM;

[0029] The normal saline solution of DMSO and β-cyclodextrin is also included in the stock solution, and the volume ratio of the normal saline solution of DMSO and β-cyclodextrin is 1:(9~19); DMSO in the stock solution The volume percentage of β-cyclodextrin is 10% to 5%, and the mass percentage of β-cyclodextrin in the stock solution is 5 to 25%;

[0030] The mass ratio of the volume of the diluent to the chitosan is 400 μl: 1-3 mg.

[0031] The present invention firstly prepares AZD2858 (3-amino-6-[4-[(4-methyl-1-piperazinyl)sulfonyl]phenyl]-N-3-pyridyl-pyrazinecarboxamide) into 2.5 ~7.5mM stock solution, the solvent of described stock solution is the mixed solution that comprises the physiological saline solution of DMSO and β-cyclodextrin; The volume ratio of DM...

Embodiment 1

[0040] 1. Preparation of AZD2858-CD-chitosan nanoparticles

[0041] 1. Add 2g of β-cyclodextrin to 10ml of normal saline to prepare 20% β-cyclodextrin in normal saline solution.

[0042] 2. Weigh 2.12 mg of AZD2858, and use a mixed solution (10% DMSO + 90% physiological saline solution containing 20% ​​β-cyclodextrin) to prepare a 2.5 mM stock solution.

[0043] 3. Take 25 μl stock solution + 975 μl 20% β-cyclodextrin physiological saline solution, and dilute to 50 nM.

[0044] 4. Weigh 1.5 mg chitosan and disperse it in 400 μl of 50 nM solution to obtain a solution containing AZD2858-CD-chitosan nanoparticles. The solution is dropped on the mica sheet, and after the solvent is evaporated to dryness, it is detected under a scanning electron microscope, and it can be seen that the AZD2858-CD-chitosan nanoparticle is a uniform particle structure ( figure 1 Middle A).

[0045] 2. Preparation of AZD2858-CD-chitosan@Matrix Gel

[0046] Add 100 μL Matrix Gel (BD Company, 10 mL) ...

Embodiment 2

[0051] Effects of AZD2858 and AZD-CD-chitosan on migration ability of apical papilla stem cells (SCAP)

[0052] SCAP was isolated and cultured in vitro, according to the cell density of 1×10 4 One per well was inoculated in the upper chamber of the transwell co-culture system, and non-specific regulatory bioactive factors amelogenin (AML), AZD2858 and AZD2858-CD-chitosan nanoparticles were added to the lower chamber of the co-culture system of each group. After 24 hours of culture, the migrating SCAP was digested, collected and counted. The results showed that AZD2858 and AZD-CD2858-chitosan nanoparticles could significantly promote the migration ability of SCAP, and there was no significant difference between the AZD2858 and AZD2858-CD-chitosan nanoparticles groups ( figure 2 ).

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Abstract

The invention provides nano-particles and gel for promoting dentin regeneration as well as a preparation method and application of the nano-particles and the gel, and relates to the technical field of biological medicines. The AZD2858-CD-chitosan nano-particles for promoting dentin regeneration are prepared by taking a diluent of a stock solution containing AZD2858 and chitosan as raw materials, and the prepared AZD2858-CD-chitosan nano-particles can promote stem cell migration of root apical papilla, dentin differentiation and development and regeneration of dental tissues. The invention further discloses AZD2858-CD-chitosan gel prepared on the basis of the AZD2858-CD-chitosan nano particles, the AZD2858-CD-chitosan gel is a sustained release preparation, and the AZD2858-CD-chitosan gel is filled in an experimental animal model, has the activity of promoting dentin regeneration, has remarkable superiority in dental pulp tissue engineering and has a huge application market in development and regeneration of dental tissues.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a nano particle and a gel for promoting dentin regeneration and a preparation method and application thereof. Background technique [0002] Pulp infectious diseases such as pulp disease and periapical disease are the most common oral diseases, with a high prevalence rate and a wide range of ages, and can even lead to tooth loss, seriously affecting the oral and general health of patients [1] . At present, the main method of clinical treatment of pulp disease and periapical disease is root canal therapy. Due to the fact that bacteria can colonize in the dentinal tubules and the anatomical structure of the root canal system is complex, it is difficult to completely remove the bacteria in the root canal and completely fill it tightly. In recent years, the overall clinical success rate of root canal treatment is 78% to 98%. [2] . In addition, root canal therapy is...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/50A61L27/54
CPCA61L27/20A61L27/50A61L27/54A61L2300/412A61L2300/602A61L2300/802A61L2400/12C08L5/16C08L5/08C08L5/06
Inventor 周建王松灵王玉记张晨胡磊贾翌江
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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