Novel calcium chelating peptide as well as preparation method and application thereof

A new type of calcium chelation technology, applied in the field of new calcium chelating peptides and their preparation, to achieve the effect of improving absorption and bioavailability, simple operation, and promoting calcium transport

Active Publication Date: 2020-01-10
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes an improved type of protein called calcium that helps keep people healthy from losing their balance due to lacking or insufficient amount of certain minerals like magnesium (Mg). Calcium plays several important roles during life such as promoting blood clotting, improving muscle function, regulating gene expression, controlling how fast your heart works properly, maintaining good circulatory system, etc., making them valuable tools when needed.

Problems solved by technology

This patents discuss how different types of food called seafood contain valuable ingredients like proteins or other substances needed during various stages of life: they include animal products made up mostly of shellfish skeleton, but also small amounts of these materials used commercially throughout this period. There is increasing interest among individuals who want healthier choices towards seafoods containing more beneficial compounds than traditional ones because many seafoaches lack effective supplementation options. Additionally, finding ways to make them easier to use without harmful side effects remains challenging despite advancements in technology related to producing biological active agents.

Method used

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  • Novel calcium chelating peptide as well as preparation method and application thereof
  • Novel calcium chelating peptide as well as preparation method and application thereof
  • Novel calcium chelating peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This embodiment provides a calcium chelating peptide whose amino acid sequence is: YQEPVIAPKL().

[0054] The calcium chelating peptide can be prepared by the following preparation method:

[0055] (1) Extraction of tilapia bone collagen

[0056] (1) Cleaning of tilapia skeleton raw materials

[0057] After the fish skeleton is thawed, impurities are removed and cleaned with tap water, and finally the fish skeleton is decapitated.

[0058] (2) De-meat treatment of tilapia skeleton

[0059] Cut the fish skeleton into about 5 cm, take 1 kg of raw material, add 5 g of neutral protease and 1000 mL of water, and carry out enzymatic hydrolysis on the fish skeleton for 2 hours (55°C, pH 7.5) to remove the minced meat attached to the bone. Impurities are washed away after enzymatic hydrolysis.

[0060] (3) Alkaline Elution Degreasing of Tilapia Bones

[0061] Put the fish bones into 0.1mol / L NaOH solution (w / v1:10) and stir for 24h, and wash until neutral.

[0062] (4) De...

Embodiment 2

[0131] In this example, the calcium chelating ability of the calcium chelating peptide and its effect on the calcium transport of the Caco-2 cell monolayer were studied, and the specific process was as follows.

[0132] (1) Peptide synthesis

[0133] Synthetic peptides (YQEPVIAPKL) with a purity higher than 95% were obtained from Synpeptide Co., Ltd (Nanjing, China), and their molecular weight was determined by LC / ESI-MS, which was 1157.627 Da.

[0134] Table 4 shows the homologous proteins of calcium chelating peptides.

[0135] Homologous proteins of table 4 calcium-chelating peptides

[0136]

[0137] The calcium sequestration activity of the synthetic peptides was determined by using the o-cresolphthalein colorimetric method. The calcium chelating activity of YQEPVIAPKL was 28.4 mg / g, indicating that the shorter peptide YQEPVIAPKL has more calcium chelating sites.

[0138] (2) Cell culture

[0139] Caco-2 cells were purchased from American Type Culture Collection (R...

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Abstract

The invention relates to novel calcium chelating peptide as well as a preparation method and an application thereof. According to the invention, an amino acid sequence of the calcium chelating peptideis YQEPVIAPKL. The calcium chelating peptide provided by the invention is safe and non-toxic, and has better physicochemical activity compared with a traditional calcium supplement, and the calcium chelating capability of the calcium chelating peptide reaches 28.4 mg/g; the collagen peptide can be supplemented while absorption and bioavailability of calcium in a human body are improved, absorption and transport of calcium in Caco-2 small intestinal epithelial cells can be promoted, and the collagen peptide can be used as a raw material for drug development and biological calcium supplement. According to the preparation method provided by the invention, the calcium chelating peptide is successfully obtained, fish product resources can be fully utilized, the operation is simple, and a new way is provided for high-valued utilization of tilapia bones.

Description

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Claims

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Application Information

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Owner SOUTH CHINA AGRI UNIV
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