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Marine animal semen collection method

A marine animal and semen collection technology, which is applied in animal delivery, medical science, veterinary instruments, etc., can solve problems such as manual collection of sperm, and achieve the effects of quality assurance, convenient and effective semen collection, and perfect conservation work

Inactive Publication Date: 2020-01-17
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for semen collection of marine animals, which solves the problem of difficulty in artificially collecting sperm in the breeding process of marine animals, provides important technical support for artificial breeding of marine animals, and breaks through the bottleneck of artificial breeding of marine animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Method steps of the present invention are as follows:

[0015] Targeting mature male marine mammals, adopting the method of artificial simulation induction to obtain sperm;

[0016] S1, by stimulating the visual and tactile senses of the marine mammals to be semen-collected, causing the animals to be semen-collected to be in estrus;

[0017] S2, choose to use a sexually mature female marine animal or a simulated sperm collection device to stimulate the animal to be collected, so that the animal to be collected will naturally ejaculate;

[0018] The ambient temperature in the simulated semen collection device is 10°C, the semen collection water temperature is 10°C, the pH value is 7.5, and the salinity is 23‰;

[0019] A buffer solution is injected into the simulated semen collection device, and the formula of the buffer solution is: glycerin 5ml, sodium chloride 1.8g, glucose 60g, potassium chloride 0.7g, trisodium citrate 3.7g, magnesium sulfate 0.3g, carbonic acid P...

Embodiment 2

[0021] Method steps of the present invention are as follows:

[0022] Targeting mature male marine mammals, adopting the method of artificial simulation induction to obtain sperm;

[0023] S1, by stimulating the visual and tactile senses of the marine mammals to be semen-collected, causing the animals to be semen-collected to be in estrus;

[0024] S2, choose to use a sexually mature female marine animal or a simulated sperm collection device to stimulate the animal to be collected, so that the animal to be collected will naturally ejaculate;

[0025] The ambient temperature in the simulated semen collection device is 28°C, the semen collection water temperature is 15°C, the pH value is 8.3, and the salinity is 35‰;

[0026] A buffer solution is injected into the simulated semen collection device, and the formula of the buffer solution is: glycerin 5ml, sodium chloride 1.8g, glucose 60g, potassium chloride 0.7g, trisodium citrate 3.7g, magnesium sulfate 0.3g, carbonic acid P...

Embodiment 3

[0028] Method steps of the present invention are as follows:

[0029] Targeting mature male marine mammals, adopting the method of artificial simulation induction to obtain sperm;

[0030] S1, by stimulating the visual and tactile senses of the marine mammals to be semen-collected, causing the animals to be semen-collected to be in estrus;

[0031] S2, choose to use a sexually mature female marine animal or a simulated sperm collection device to stimulate the animal to be collected, so that the animal to be collected will naturally ejaculate;

[0032] The ambient temperature in the simulated semen collection device is 20°C, the semen collection water temperature is 13°C, the pH value is 7.9, and the salinity is 28‰;

[0033] A buffer solution is injected into the simulated semen collection device, and the formula of the buffer solution is: glycerin 5ml, sodium chloride 1.8g, glucose 60g, potassium chloride 0.7g, trisodium citrate 3.7g, magnesium sulfate 0.3g, carbonic acid P...

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PUM

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Abstract

The invention discloses a marine animal semen collection method. Sperms are obtained by a manual simulating and inducing method for sexual maturity marine mammals, the visual and the touch sense of the marine mammals of which the sperms are to be collected are stimulated, and animals of which the sperms are to be collected are in oestrus. The sexual maturity marine mammals are selected and used ora simulation semen collector is used for stimulating the animals of which the sperms are to be collected, so that the animals of which the sperms are to be collected can realize natural spermiation.The environmental temperature in the simulation semen collector is 10-28 DEG C, the semen collection water temperature is 10-15 DEG C, the pH is 7.5-8.3 and the salinity is 2.3-3.5%. Buffer liquid isinjected into the simulation semen collector, and adopts the formula of 5ml of glycerine, 1.8g of sodium chloride, 60g of glucose, 0.7g of potassium chloride, 3.7g of trisodium citrate, 0.3g of magnesium sulphate, 1.2g of potassium bicarbonate, 0.6g of cow's serum and 500ug of gentamycin. According to the method, the semen collection operation is convenient and effective, the semen collection process cannot cause damage and negative stimulus to the marine animals, and the obtained sperms are high in quality.

Description

technical field [0001] The invention relates to the field of marine mammal breeding, in particular to a method for semen collection of marine animals. Background technique [0002] Marine animals are distributed in the world's oceans from the north and south poles to the equator, and are important aquatic animals. However, the global environment is changing day by day, and the survival of marine animals is seriously threatened. In recent years, the number of marine animals in nature has declined sharply, and they have been included in the "IUCN Red List of Threatened Species" and "Convention on International Trade in Endangered Species of Wild Fauna and Flora". "; in response to this severe situation, animal conservation institutions and aquariums around the world have carried out a series of protection measures for marine animals in recent years; while our country belongs to the ex situ protection model. Moreover, the reproduction rate of marine animals is low, the reprodu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/02
CPCA61D19/021
Inventor 王伟罗珺韩雨哲张赛赛陈博锦孙鹏飞王一诺
Owner DALIAN OCEAN UNIV
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