Preparation method and application of polypeptide liposome

A technology of peptide lipids and liposomes, which is applied in the field of preparation of polypeptide liposomes, can solve problems such as unstable performance and unsatisfactory skin care effects, and achieve the effects of reducing water evaporation, good thickening, and increasing skin elasticity Effect

Active Publication Date: 2020-01-21
深圳市一支秀美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current skin care products inevitably need to contact the external environment before penetrating into the epidermis, the performance is unstable, and the skin care effect is not ideal

Method used

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  • Preparation method and application of polypeptide liposome
  • Preparation method and application of polypeptide liposome
  • Preparation method and application of polypeptide liposome

Examples

Experimental program
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Embodiment 1

[0046] The embodiment of the present invention discloses a preparation method of polypeptide liposome, which specifically includes the following steps (refer to Table 1 for each composition and content):

[0047] S1 takes hydrogenated lecithin, cholesterol, coco-sulfobetaine and vitamin E, dissolves them in chloroform solution, and ultrasonically treats them at an input power of 30% for 8-10 minutes, then evaporates the chloroform solution completely at room temperature to obtain Blank liposome film and place it in a vacuum drying oven to dry for 3h;

[0048] S2 Take deionized water to dissolve the dry blank liposome film in step S1 to obtain an aqueous solution, and ultrasonically treat the aqueous solution at an input power of 20% for 8-10 minutes, and squeeze the aqueous solution through 0.6 μm, 0.45 μm and 0.22 μm in sequence A polycarbonate membrane is filtered to obtain a blank liposome solution;

[0049] S3 slowly add tripeptide-1, palmitoyl tripeptide-5, palmitoyl tet...

Embodiment 2

[0052] The preparation method of a polypeptide liposome disclosed in the embodiment of the present invention (refer to Table 1 for each composition and content), is basically the same as the preparation steps of Example 1, the difference is:

[0053] In step S1, the vacuum drying time of the blank liposome film is 4 hours; in step S3, the incubation time is 30 minutes; in step S4, after adding trehalose, pre-freeze at -40°C for 2 hours, and then vacuumize Freeze-dry for 24 hours under the same conditions, and store at 4°C to obtain polypeptide liposomes.

Embodiment 3

[0055] The preparation method of a polypeptide liposome disclosed in the embodiment of the present invention (refer to Table 1 for each composition and content), is basically the same as the preparation steps of Example 1, the difference is:

[0056] In step S1, the vacuum drying time of the blank liposome film is 5 h; in step S3, the incubation time is 40 min; in step S4, after adding trehalose, pre-freeze at -50°C for 3 h, and then vacuumize Freeze-drying under conditions for 48 hours and storing at 7°C to obtain polypeptide liposomes.

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Abstract

The invention provides a preparation method and application of a polypeptide liposome. The method comprises the following steps: respectively weighing hydrogenated lecithin, cholesterol and coconut oil-sulfobetaine with a weight ratio of (6.5-7):(1.6-2):(0.5-1) and 0.5-1.5 parts by weight of vitamin E, dissolving the materials in a chloroform solution, evaporating the chloroform solution completely at a room temperature to obtain a blank liposome film, and performing drying; dissolving the dried blank liposome film in deionized water to obtain an aqueous solution, and performing filtration toobtain a blank liposome solution; slowly adding a polypeptide substance into the blank liposome solution, performing slight stirring at room temperature, performing incubation for 20-40min, and performing filtration to obtain liposome filtrate; and adding 0.5-4 parts by weight of trehalose into the liposome filtrate, and performing freeze-drying to obtain the polypeptide liposome. The preparationmethod encapsulates the polypeptide substance by using the liposome, so that the prepared polypeptide liposome has good stability and good moisturizing effect.

Description

technical field [0001] The invention relates to the technical field of liposome preparation, in particular to a preparation method and application of polypeptide liposome. Background technique [0002] With age, under the influence of various factors (for example, the constant pull of gravity, frequent and continuous positional pressure of the facial skin (such as during sleep) or repetitive facial movements caused by the contraction of facial expression muscles, etc.), Facial skin begins to age, becoming sagging, thinner, dull, less hydrated, and less elastic. Facial skin aging is directly related to changes in the triple helix structure of collagen, degradation of elastin polypeptides, and disordered arrangement of the skin lipid matrix. Therefore, when the facial expression muscles are relaxed, the facial skin cannot recover quickly, and wrinkles will increase and deepen over time. [0003] Among them, facial skin is composed of epidermis and dermis, and the deep layer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/14A61K8/46A61K8/55A61K8/60A61K8/63A61K8/67A61Q19/00A61Q19/08
CPCA61K8/466A61K8/678A61K8/553A61K8/63A61K8/60A61K8/64A61K8/14A61Q19/00A61Q19/08A61K2800/84A61K2800/82
Inventor 刘忠张红珊黄毅洲林翀
Owner 深圳市一支秀美生物科技有限公司
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