Method of detecting molecular weight of pneumococcal capsular poly-saccharides
A technology of capsular polysaccharide and pneumococcus, applied in the biological field, can solve the problems of poor repeatability, time-consuming, labor-consuming, etc., and achieve the effects of high accuracy, good repeatability and improved accuracy
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Embodiment 1
[0046] 1) Instruments and reagents
[0047]JA2003 electronic balance was purchased from Shanghai Sunny Hengping Scientific Instrument Co., Ltd.; DV2T viscometer was purchased from BROOK FIELD company, rotor model: CPA-40Z; e2695 high-efficiency liquid phase system pump, Waters 2414 differential refraction detector were purchased from Waters company; The high-performance liquid chromatography column is TSK PWXL Guard protection column and TSK G5000PWXL (7.8mmID×30cm) analysis column purchased from Japan TOSOH Corporation.
[0048] Reagents: Sodium dihydrogen phosphate dodecahydrate, disodium hydrogen phosphate dihydrate, sodium chloride, and dextran standards were purchased from Waters and American Polymer Standards Corporation.
[0049] Preparation of 0.1mol / L phosphate buffer solution: Weigh 0.4g sodium dihydrogen phosphate dodecahydrate, 6.0g disodium hydrogen phosphate dihydrate, 9.0g sodium chloride, add deionized water to 1000g.
[0050] 2) Preparation of samples for ser...
Embodiment 2
[0057] 1) Instruments and reagents
[0058] JA2003 electronic balance was purchased from Shanghai Sunny Hengping Scientific Instrument Co., Ltd.; DV2T viscometer was purchased from BROOK FIELD company, rotor model: CPA-40Z; e2695 high-efficiency liquid phase system pump, Waters 2414 differential refraction detector were purchased from Waters company; The high-performance liquid chromatography column is TSK PWXL Guard protection column and TSK G5000PWXL (7.8mmID×30cm) analysis column purchased from Japan TOSOH Corporation.
[0059] Reagents: Sodium dihydrogen phosphate dodecahydrate, disodium hydrogen phosphate dihydrate, sodium chloride, and dextran standards were purchased from Waters and American Polymer Standards Corporation.
[0060] Preparation of 0.1mol / L phosphate buffer solution: Weigh 0.4g sodium dihydrogen phosphate dodecahydrate, 6.0g disodium hydrogen phosphate dihydrate, 9.0g sodium chloride, add deionized water to 1000g.
[0061] 2) Preparation of 33F serotype P...
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