Fermentation method for high-yield beta-glucan based on sparassis crispa

A fermentation method, Hydrangea technology, applied in the field of fermentation based on Hydrangea high-yield β-glucan, can solve the problems of limited glucan content, low extraction rate, etc., to reduce external restrictions, improve purity, and promote bacterial flora Effect

Active Publication Date: 2020-02-11
FUQING CITY FIRE KIRIN EDIBLE FUNGUS TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, with more and more attention and applications from all walks of life, the demand for dextran is increasing year by year, an

Method used

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  • Fermentation method for high-yield beta-glucan based on sparassis crispa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] S1: Activation of strains, the temperature of strain activation is 25-30°C, static culture at constant temperature for 2-3 days, inoculation of hydrangea strains on the strain activation medium;

[0029] S2: Preparation of the primary medium, the primary medium is based on yeast powder 5.0-8.0g / L, starch 8.0-10.0 g / L, peptone 10.0-15.0g / L, biotin 0.5-1.5g / L, glycerin 10.0-13.0 The mass concentration of g / L is equipped;

[0030] S3: Preparation of hydrangea primary seed liquid; scrape hydrangea species from the strain activation medium and transfer them to the primary medium for cultivation. After inoculation, it needs to be shaken and cultured in a shaker at a speed of 200-280rpm for 1-2d , the culture temperature is 25-30°C, the OD600 value of the culture solution is between 0.6-0.9, and the primary seed solution of hydrangea is obtained;

[0031] S4: preparation of two-way culture seed solution; adding two-way fermentation culture strains to the hydrangea primary cul...

Embodiment 2

[0037] S1: Activation of strains, the temperature of strain activation is 25-30°C, static culture at constant temperature for 2-3 days, inoculation of hydrangea strains on the strain activation medium;

[0038] S2: Preparation of the primary medium, the primary medium is based on yeast powder 5.0-8.0g / L, starch 8.0-10.0 g / L, peptone 10.0-15.0g / L, biotin 0.5-1.5g / L, glycerin 10.0-13.0 The mass concentration of g / L is equipped;

[0039] S3: Preparation of hydrangea primary seed liquid; scrape hydrangea species from the strain activation medium and transfer them to the primary medium for cultivation. After inoculation, it needs to be shaken and cultured in a shaker at a speed of 200-280rpm for 1-2d , the culture temperature is 25-30°C, the OD600 value of the culture solution is between 0.6-0.9, and the primary seed solution of hydrangea is obtained;

[0040]S4: preparation of two-way culture seed solution; adding two-way fermentation culture strains to the hydrangea primary cult...

Embodiment 3

[0046] S1: Activation of strains, the temperature of strain activation is 25-30°C, static culture at constant temperature for 2-3 days, inoculation of hydrangea strains on the strain activation medium;

[0047] S2: Preparation of the primary medium, the primary medium is based on yeast powder 5.0-8.0g / L, starch 8.0-10.0 g / L, peptone 10.0-15.0g / L, biotin 0.5-1.5g / L, glycerin 10.0-13.0 The mass concentration of g / L is equipped;

[0048] S3: Preparation of hydrangea primary seed liquid; scrape hydrangea species from the strain activation medium and transfer them to the primary medium for cultivation. After inoculation, it needs to be shaken and cultured in a shaker at a speed of 200-280rpm for 1-2d , the culture temperature is 25-30°C, the OD600 value of the culture solution is between 0.6-0.9, and the primary seed solution of hydrangea is obtained;

[0049] S4: preparation of two-way culture seed solution; adding two-way fermentation culture strains to the hydrangea primary cul...

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Abstract

The invention discloses a fermentation method for high-yield beta-glucan based on sparassis crispa. According to the method, by means of multistage amplification treatment, the growth range of the sparassis crispa is expanded as far as possible, the external limit is reduced, and nutrients required by fermentation growth are provided sufficiently; the extraction rate of glucan is increased by gastrodia elata extract, due to the fact that the gastrodia elata extract contains a large amount of gastrodin, ethanolic extract of gastrodia elata and p-hydroxybenzaldehyde, the substances can promote growth of mycelia, and meanwhile, key components for glucan synthesis are provided; and content of protein and starch in a final product can be reduced through two-way fermentation, so that the purityof glucan is improved, meanwhile, the flora promotion effect can be achieved, and the growth of the sparassis crispa is further improved.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a fermentation method based on hydrangea high-yield β-glucan. Background technique [0002] Among the glucans, β-glucan is the most physiologically active. The active structure of β-glucan is a polysaccharide composed of glucose units, most of which are bound by β-1,3, which is how the glucose chains are connected. It can activate macrophages, neutrophils, etc., so it can increase the content of leukin, cytokinin and special antibodies, and fully stimulate the body's immune system. Then, the body is more prepared to resist diseases caused by microorganisms. β-glucan can quickly restore the ability of the injured body's lymphocytes to produce cytokines (IL-1), and effectively regulate the body's immune function. β-glucan can promote the production of IgM antibody in the body to improve the immunity of humoral fluid. This kind of glucan-activated cells can stimu...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N1/14C12R1/645
CPCC12N1/14C12N9/244C12Y302/01006
Inventor 陈美英王国强何绍东
Owner FUQING CITY FIRE KIRIN EDIBLE FUNGUS TECH DEV
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