A kind of fermentation method based on Hydrangea high-yield β-glucan

A fermentation method and Embroidered bacteria technology are applied in the field of fermentation based on Embroidered Coccus high-yield β-glucan, which can solve the problems of limited glucan content and low extraction rate.

Active Publication Date: 2021-06-11
FUQING CITY FIRE KIRIN EDIBLE FUNGUS TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, with more and more attention and applications from all walks of life, the demand for dextran is increasing year by year, and the supply is in short supply, mainly because the content of dextran in its raw materials is limited, resulting in a low extraction rate.

Method used

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  • A kind of fermentation method based on Hydrangea high-yield β-glucan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] S1: Activation of strains, the temperature of strain activation is 25-30°C, static culture at constant temperature for 2-3 days, inoculation of hydrangea strains on the strain activation medium;

[0029] S2: Preparation of the primary medium, the primary medium is based on yeast powder 5.0-8.0g / L, starch 8.0-10.0 g / L, peptone 10.0-15.0g / L, biotin 0.5-1.5g / L, glycerin 10.0-13.0 The mass concentration of g / L is equipped;

[0030] S3: Preparation of hydrangea primary seed liquid; scrape hydrangea species from the strain activation medium and transfer them to the primary medium for cultivation. After inoculation, it needs to be shaken and cultured in a shaker at a speed of 200-280rpm for 1-2d , the culture temperature is 25-30°C, the OD600 value of the culture solution is between 0.6-0.9, and the primary seed solution of hydrangea is obtained;

[0031] S4: preparation of two-way culture seed solution; adding two-way fermentation culture strains to the hydrangea primary cul...

Embodiment 2

[0037] S1: Activation of strains, the temperature of strain activation is 25-30°C, static culture at constant temperature for 2-3 days, inoculation of hydrangea strains on the strain activation medium;

[0038] S2: Preparation of the primary medium, the primary medium is based on yeast powder 5.0-8.0g / L, starch 8.0-10.0 g / L, peptone 10.0-15.0g / L, biotin 0.5-1.5g / L, glycerin 10.0-13.0 The mass concentration of g / L is equipped;

[0039] S3: Preparation of hydrangea primary seed liquid; scrape hydrangea species from the strain activation medium and transfer them to the primary medium for cultivation. After inoculation, it needs to be shaken and cultured in a shaker at a speed of 200-280rpm for 1-2d , the culture temperature is 25-30°C, the OD600 value of the culture solution is between 0.6-0.9, and the primary seed solution of hydrangea is obtained;

[0040]S4: preparation of two-way culture seed solution; adding two-way fermentation culture strains to the hydrangea primary cult...

Embodiment 3

[0046] S1: Activation of strains, the temperature of strain activation is 25-30°C, static culture at constant temperature for 2-3 days, inoculation of hydrangea strains on the strain activation medium;

[0047] S2: Preparation of the primary medium, the primary medium is based on yeast powder 5.0-8.0g / L, starch 8.0-10.0 g / L, peptone 10.0-15.0g / L, biotin 0.5-1.5g / L, glycerin 10.0-13.0 The mass concentration of g / L is equipped;

[0048] S3: Preparation of hydrangea primary seed liquid; scrape hydrangea species from the strain activation medium and transfer them to the primary medium for cultivation. After inoculation, it needs to be shaken and cultured in a shaker at a speed of 200-280rpm for 1-2d , the culture temperature is 25-30°C, the OD600 value of the culture solution is between 0.6-0.9, and the primary seed solution of hydrangea is obtained;

[0049] S4: preparation of two-way culture seed solution; adding two-way fermentation culture strains to the hydrangea primary cul...

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Abstract

The invention discloses a fermentation method based on Hydrangea high-yield β-glucan. The method may expand the growth range of Hydrangea through multi-stage amplification treatment, reduce external restrictions, and fully provide nutrients required for fermentation growth; Gastrodia elata extract increases the extraction rate of glucan, because Gastrodia elata extract contains a large amount of gastrodin, gastrodin alcohol and p-hydroxybenzaldehyde, these substances can promote the growth of mycelium, and are also key components of glucan synthesis; Through two-way fermentation, the protein and starch content in the final product can be reduced, thereby improving the purity of dextran, and at the same time, it can also play a role in promoting the flora to further increase the growth of hydrangea.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a fermentation method based on hydrangea high-yield β-glucan. Background technique [0002] Among the glucans, β-glucan is the most physiologically active. The active structure of β-glucan is a polysaccharide composed of glucose units, most of which are bound by β-1,3, which is how the glucose chains are connected. It can activate macrophages, neutrophils, etc., so it can increase the content of leukin, cytokinin and special antibodies, and fully stimulate the body's immune system. Then, the body is more prepared to resist diseases caused by microorganisms. β-glucan can quickly restore the ability of the injured body's lymphocytes to produce cytokines (IL-1), and effectively regulate the body's immune function. β-glucan can promote the production of IgM antibody in the body to improve the immunity of humoral fluid. This kind of glucan-activated cells can stimu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N1/14C12R1/645
CPCC12N1/14C12N9/244C12Y302/01006
Inventor 陈美英王国强何绍东
Owner FUQING CITY FIRE KIRIN EDIBLE FUNGUS TECH DEV
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