Method for reducing TSNAs in tobacco leaves of cigar by utilizing strains

A technology of cigars and tobacco leaves, which is applied in the field of tobacco industry, can solve the problems that the overall effect is not very obvious, and achieve the effects of improving survival rate and activity, convenient operation, and low cost of use

Active Publication Date: 2020-02-14
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the predecessors have explored ways to reduce nitrosamines (TSNAs) in tobacco leaves in many ways, most of which involve flue-cured tobacco, cigarettes, etc., including the use of equipment, tobacco leaf preparation, and improved technology, etc., but the overall effect is not very obvious. The method of degradation of cigar tobacco nitrosamines (TSNAs) is rarely reported, therefore, reducing cigar tobacco TSNAs by inoculating bacteria is a direction worthy of research

Method used

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  • Method for reducing TSNAs in tobacco leaves of cigar by utilizing strains
  • Method for reducing TSNAs in tobacco leaves of cigar by utilizing strains
  • Method for reducing TSNAs in tobacco leaves of cigar by utilizing strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] S100: Harvest the upper leaves, middle leaves and lower leaves of tobacco plants with suitable maturity in Gengma County, Lincang City, Yunnan Province, wherein:

[0039] The lower leaves are light green or slightly yellow in green, the main vein is white, the branch veins are tender white, the leaf surface is smooth and shiny with greenish yellow, part of the glandular hairs has fallen off, and the tip of the leaf is slightly withered and yellow.

[0040] The middle leaves are collected in light green or yellowish green, with bright white main veins and light white branch veins. The leaf surface is slightly wrinkled and shiny greenish yellow, with mature spots, leaf tips and margins drooping, glandular hairs basically fall off, and the angle of stems and leaves increases. Large tobacco leaves.

[0041] The upper leaves are yellow-green or greenish-yellow, the main veins are white and bright, the branches are white, the leaf surface is wrinkled and shiny yellow-green, m...

Embodiment 2

[0052] On the basis of Example 1, 05-101, J45, J54 and mixed strains were inoculated during the drying process of cigars, and their effects on the degradation of nitrosamines (TSNAs) in cigars were determined.

[0053] Experiment 1 process:

[0054] Cultivate the 05-101, J45, and J54 strains obtained through the separation, screening and purification processes at 25-30°C for 60 hours to obtain a fermentation agent, and then mix the fermentation agent with sterilized water to obtain OD 600 Bacteria with a value of 1. In the air-curing process of cigars, 4% bacterial liquid by weight of tobacco leaves is sprayed evenly on the tobacco leaves in turn at the beginning of the air-curing yellowing period and the beginning of the color-fixing period for inoculation.

[0055] This experiment set up 4 treatments (TI, T2, T3, T4, CK), among which: the non-inoculated strain in Example 1 was used as the control (CK); the T1 bacterial solution was prepared with 05-101 strain; the T2 bacter...

Embodiment 3

[0059] On the basis of Example 1, 05-101, J45, J54 and mixed strains were inoculated during the fermentation of cigars, and their effects on the degradation of nitrosamines (TSNAs) in cigars were determined.

[0060] Experiment 2 process:

[0061] Cultivate the 05-101, J45, and J54 strains obtained through the separation, screening and purification processes at 25-30°C for 60 hours to obtain a fermentation agent, and then mix the fermentation agent with sterilized water to obtain OD 600 Bacteria with a value of 1. During the fermentation process of cigars, 4% bacterial liquid by weight of tobacco leaves was evenly sprayed on the tobacco leaves for inoculation at the beginning of fermentation and 21 days of fermentation respectively.

[0062] This experiment set up 4 treatments (TI, T2, T3, T4, CK), among which: the non-inoculated strain in Example 1 was used as the control (CK); the T1 bacterial solution was prepared with 05-101 strain; the T2 bacterial solution was prepared ...

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Abstract

The invention discloses a method for reducing TSNAs in tobacco leaves of a cigar by utilizing strains. The method comprises the steps of: harvesting fresh tobacco leaves which are proper in maturity;drying the harvested fresh tobacco leaves in an air-drying shelter step by step, and in the drying process, inoculating in twice by bacterial solution with an OD600 value of 1, which is prepared fromat least one of strains 05-101, J45 and J54; and fermenting the air-dried tobacco leaves by adopting centralization stacking, and in the fermenting process, inoculating in twice by the bacterial solution with the OD600 value of 1, which is prepared from at least one of strains 05-101, J45 and J54 so as to obtain low-TSNAs cigar tobacco leaves. According to the invention, three strains capable of obviously reducing specific TSNAs in the cigar are selected, the single or mixed strains are fermented to prepare the bacterial solution and the bacterial solution is applied to the cigar air-drying and fermenting process, and temperature and humidity control in the air-drying and fermenting process is combined, so that high-efficiency directional degradation on the specific TSNAs in the cigar is implemented; and the method is good in stress resistance and simple and convenient to operate and has high popularization and application value.

Description

technical field [0001] The invention belongs to the technical field of the tobacco industry, and in particular relates to a method for reducing TSNAs of cigar tobacco leaves by using bacterial strains which is easy to operate, can efficiently and directionally degrade the TSNAs content of cigar tobacco, and has good stress resistance. Background technique [0002] Nitrosamines (TSNAs) are tobacco-specific N-nitrosamine-based compounds, which are harmful substances in tobacco and one of the main harmful substances that affect human health. Among the eight TSNAs identified so far, N-nitrosonornicotine (NNN), 4-(N-methyl-nitrosamine)-1-(3-pyridyl)-1-butane The four kinds of ketones (NNK), N-nitrosoanatabine (NAT) and N-nitrosoanabatin (NAB) were higher in content. As early as 1962, the potential carcinogenic effects of tobacco-specific nitrosamines (TSNAs) were reported for the first time in the world, leading to the formation of tumors in human lungs, mouth, esophagus, stomac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 胡彬彬胡梦阳邹聪明姜永雷朱艳梅夏振远陈颐蔺忠龙陈丹赵高坤何鲜苏家恩徐安传巩江世琪何元胜吴玉萍孔光辉晋艳李永平
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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