A roasting method for reducing tsnas of different varieties of flue-cured tobacco by using strains

A curing method and strain technology, applied in the fields of tobacco, application, and treatment of tobacco, can solve the problems of reducing tobacco leaves, achieve the effects of reducing harm, increasing survival rate and activity, and expanding the scope of use

Active Publication Date: 2021-07-23
YUNNAN ACAD OF TOBACCO AGRI SCI
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although predecessors have explored ways to reduce TSNAs in tobacco leaves from various aspects such as agricultural technology, modulation technology, and biotechnology, although the method of using bacteria to degrade TSNAs in the prior art is useful for directional cleaning of pollutants and purification of drinking water, etc. There are also bacterial liquids used in tobacco for drying and sedimentation of Burley tobacco, but it is rare to use bacterial strains to reduce TSNAs in tobacco leaves during the curing process, and there are few reports on methods for reducing TSNAs content in specific varieties of flue-cured tobacco

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A roasting method for reducing tsnas of different varieties of flue-cured tobacco by using strains
  • A roasting method for reducing tsnas of different varieties of flue-cured tobacco by using strains
  • A roasting method for reducing tsnas of different varieties of flue-cured tobacco by using strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Effect of the present invention on the content of TSNAs in Honghua Dajinyuan tobacco leaves

[0046] S100: Harvest the fresh flue-cured tobacco leaves of Honghua Dajinyuan with suitable maturity in Xiaguan Town, Dali Prefecture, Yunnan Province. The fresh tobacco leaves with suitable maturity have white and bright main veins, green and white branches, and green-yellow or light yellow leaves. And most of the fluff falls off, and it has the characteristics of rich e-liquid after touching it.

[0047] S200: classifying the harvested fresh tobacco leaves according to the amount of 90-110 pieces / stem to be compiled into stems.

[0048] S300: 3-5% of the weight of the tobacco leaves is evenly sprayed and inoculated on the surface of the woven tobacco leaves with a bacterial solution with an OD600 value of 1.

[0049] S400: Put the inoculated woven stem tobacco leaves into the air-flow descending intensive curing barn to more than 95% of the volume according to the ...

Embodiment 2

[0058] Example 2 Effect of conventional curing process on TSNAs content in Honghua Dajinyuan tobacco leaves

[0059] On the basis of Example 1, a control experiment was set up: in Example 1, the wet bulb temperature in the mid-yellowing period was adjusted to 36°C, and the baking time was adjusted to 22h; the wet bulb temperature in the late yellowing period was adjusted to 37°C, and the baking time Adjusted to 14h; other process parameters remain unchanged.

[0060] Table 2 Comparison of TSNAs content in flue-cured tobacco varieties of Honghua Dajinyuan after conventional roasting

[0061]

[0062] It can be seen from Table 1 that the TSNAs content of flue-cured tobacco in Honghua Dajinyuan reduced by 21.29-23.97% under the optimized process of the present invention; In the case of inoculated strains, the reduction was 17.41-20.43%, so the inoculated strains can significantly reduce the content of TSNAs in flue-cured tobacco, and the optimized process of the present inven...

Embodiment 3

[0063] Example 3 Effects of the present invention's baking process on the activity of Honghua Dajinyuan tobacco strains

[0064] According to embodiments 1 and 2, when the initial stage of curing yellowing ends and enters the later stage of yellowing, the viable bacteria count and NO 2 - content.

[0065] Table 3 The number of effective viable bacteria and NO per unit area of ​​tobacco leaves 2 - Content comparison

[0066]

[0067] It can be seen from Table 3 that compared with the conventional process, the number of effective viable bacteria per unit area of ​​this method is obviously higher, and the precursor substance NO of its scavenging nitrosamine 2 - The ability is stronger, so it has been confirmed that this method technology can improve the survival rate of bacterial classification.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a baking method for reducing TSNAs of different varieties of flue-cured tobacco by using bacterial strains, which comprises harvesting fresh flue-cured tobacco leaves with suitable maturity; classifying the harvested fresh tobacco leaves and then weaving stems; At least one strain prepares a bacterial solution with an OD600 value of 1, and then evenly sprays the bacterial solution on the surface of the woven tobacco leaves; loads the inoculated woven tobacco leaves into a dense barn; Toasting, after the yellowing period, color fixing period and gluten drying period, the tobacco leaves with low TSNAs can be obtained. The present invention inoculates the fresh tobacco leaves to be loaded with a special bacterial liquid, and regulates the temperature and humidity stage changes in the drying room of the barn during the curing process according to the types of tobacco leaves, so as to promote the degradation of TSNAs in the flue-cured tobacco, achieve the purpose of regulating the TSNAs content of the tobacco leaves, and improving the quality of the flue-cured tobacco. Objective, it is of great significance to reduce the harm of tobacco TSNAs to human health and living environment.

Description

technical field [0001] The invention belongs to the technical field of tobacco curing, and in particular relates to a curing method for reducing TSNAs of different varieties of flue-cured tobacco by using bacterial strains, which can effectively reduce the TSNAs content of flue-cured tobacco to improve the quality of tobacco leaves. Background technique [0002] TSNAs are tobacco-specific N-nitrosamine-based compounds, and they are also one of the main harmful substances that affect human health. Among the 8 TSNAs identified so far, the 4 kinds of NNN, NAT, NAB and NNK are relatively high in content, and both NNN and NNK are strong carcinogens in animals. Confirmed in high incidence studies. Studies have shown that factors such as tobacco species, tobacco structure, tobacco cultivation, tobacco curing, soil microbial community, and fertilization amount are all related to the content of TSNAs in tobacco. In addition, a large number of studies have also shown that the conten...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A24B3/10A24B15/20
CPCA24B3/10A24B15/20
Inventor 邹聪明胡梦阳姜永雷朱艳梅夏振远张轲陈颐蔺忠龙赵高坤何鲜苏家恩徐安传宗吉建李鑫楷巩江世琪冀新威吴玉萍刘芮王涛任可李文标
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap