Curing method for reducing TSNAs in different varieties of flue-cured tobaccos by utilizing strains

A curing method and strain technology, applied in the fields of tobacco, application, and preparation of tobacco, can solve the problems of reducing tobacco leaves, achieve the effects of reducing harm, obvious effect, and improving survival rate and activity

Active Publication Date: 2020-02-14
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although predecessors have explored ways to reduce TSNAs in tobacco leaves from various aspects such as agricultural technology, modulation technology, and biotechnology, although the method of using bacteria to degrade TSNAs in the prior art is useful for directional cleaning of pollutants and purification of drinking water, etc. There are also bacterial liquids used in tobacco for drying and sedimentation of Burley tobacco, but it is rare to use bacterial strains to reduce TSNAs in tobacco leaves during the curing process, and there are few reports on methods for reducing TSNAs content in specific varieties of flue-cured tobacco

Method used

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  • Curing method for reducing TSNAs in different varieties of flue-cured tobaccos by utilizing strains
  • Curing method for reducing TSNAs in different varieties of flue-cured tobaccos by utilizing strains
  • Curing method for reducing TSNAs in different varieties of flue-cured tobaccos by utilizing strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Effect of the present invention on the content of TSNAs in Honghua Dajinyuan tobacco leaves

[0046] S100: Harvest the fresh flue-cured tobacco leaves of Honghua Dajinyuan with suitable maturity in Xiaguan Town, Dali Prefecture, Yunnan Province. The fresh tobacco leaves with suitable maturity have white and bright main veins, green and white branches, and green-yellow or light yellow leaves. And most of the fluff falls off, and it has the characteristics of rich e-liquid after touching it.

[0047] S200: classify the above harvested fresh tobacco leaves according to the amount of 90-110 pieces / stem for weaving and weaving the stems.

[0048] S300: The amount of 3-5% of the weight of the tobacco leaves is evenly sprayed and inoculated on the surface of the woven tobacco leaves with a bacterial solution with an OD600 value of 1.

[0049] S400: Put the inoculated woven stem tobacco leaves into the air-flow descending intensive curing barn to more than 95% of the...

Embodiment 2

[0058] Example 2 Effect of conventional curing process on TSNAs content in Honghua Dajinyuan tobacco leaves

[0059] On the basis of Example 1, a control experiment was set up: in Example 1, the wet bulb temperature in the mid-yellowing period was adjusted to 36°C, and the baking time was adjusted to 22h; the wet bulb temperature in the late yellowing period was adjusted to 37°C, and the baking time Adjusted to 14h; other process parameters remain unchanged.

[0060] Table 2 Comparison of TSNAs content in flue-cured tobacco varieties of Honghua Dajinyuan after conventional roasting

[0061]

[0062] It can be seen from Table 1 that the TSNAs content of flue-cured tobacco in Honghua Dajinyuan under the optimized process of the present invention is reduced by 21.29-23.97% under the condition of inoculating strains; it can be seen from Table 2 that the TSNAs content of flue-cured tobacco under the conventional technology of Honghua Dajinyuan is between 21.29 and 23.97%. In th...

Embodiment 3

[0063] Example 3 Effects of the present invention's baking process on the activity of Honghua Dajinyuan tobacco strains

[0064] According to embodiments 1 and 2, when the initial stage of curing yellowing ends and enters the later stage of yellowing, the viable bacteria count and NO 2 - content.

[0065] Table 3 The number of effective viable bacteria and NO per unit area of ​​tobacco leaves 2 - Content comparison

[0066]

[0067] It can be seen from Table 3 that compared with the conventional process, the number of effective viable bacteria per unit area of ​​this method is obviously higher, and the precursor substance NO of its scavenging nitrosamine 2 - The ability is stronger, so it has been confirmed that this method technology can improve the survival rate of bacterial classification.

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Abstract

The invention discloses a curing method for reducing TSNAs in different varieties of flue-cured tobaccos by utilizing strains. The method comprises the following steps of harvesting fresh tobacco leaves, with the proper maturity, of the flue-cured tobaccos; classifying the harvested fresh tobacco leaves and then braiding the tobacco leaves into rods; preparing a bacterial solution with the OD600 value being 1 by using at least one strain in 05-101, J45 and J54, and then uniformly spraying the bacterial solution to the surface of the tobacco leaves which have been braided into the rods; loadingthe inoculated tobacco leaves which have been braided into the rods into a bulk curing barn; and curing the tobacco leaves which are loaded in a furnace, and obtaining the tobacco leaves with the lowTSNAs content through a yellowing stage, a color fixing stage and a stem drying stage. The special bacterial solution is inoculated to the fresh tobacco leaves which are about to be loaded in the furnace, and the stage changes of the environmental temperature and humidity in a drying chamber of the curing barn in the curing process are controlled according to the varieties of the tobacco leaves,so that the degradation of the TSNAs in the flue-cured tobaccos is promoted, the purpose of controlling the TSNAs content of the tobacco leaves to improve the quality of the flue-cured tobaccos is achieved, and the great significance is achieved for reducing the harm of the TSNAs in the tobaccos to the human health and the living environment.

Description

technical field [0001] The invention belongs to the technical field of tobacco curing, and in particular relates to a curing method for reducing TSNAs of different varieties of flue-cured tobacco by using bacterial strains, which can effectively reduce the TSNAs content of flue-cured tobacco to improve the quality of tobacco leaves. Background technique [0002] TSNAs are tobacco-specific N-nitrosamine-based compounds, and they are also one of the main harmful substances that affect human health. Among the 8 TSNAs identified so far, the 4 kinds of NNN, NAT, NAB and NNK are relatively high in content, and both NNN and NNK are strong carcinogens in animals. Confirmed in high incidence studies. Studies have shown that factors such as tobacco species, tobacco structure, tobacco cultivation, tobacco curing, soil microbial community, and fertilization amount are all related to the content of TSNAs in tobacco. In addition, a large number of studies have also shown that the conten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B3/10A24B15/20
CPCA24B3/10A24B15/20
Inventor 邹聪明胡梦阳姜永雷朱艳梅夏振远张轲陈颐蔺忠龙赵高坤何鲜苏家恩徐安传宗吉建李鑫楷巩江世琪冀新威吴玉萍刘芮王涛任可李文标
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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