Curing method for reducing TSNAs in different varieties of flue-cured tobaccos by utilizing strains

Abstract

The invention discloses a curing method for reducing TSNAs in different varieties of flue-cured tobaccos by utilizing strains. The method comprises the following steps of harvesting fresh tobacco leaves, with the proper maturity, of the flue-cured tobaccos; classifying the harvested fresh tobacco leaves and then braiding the tobacco leaves into rods; preparing a bacterial solution with the OD600 value being 1 by using at least one strain in 05-101, J45 and J54, and then uniformly spraying the bacterial solution to the surface of the tobacco leaves which have been braided into the rods; loadingthe inoculated tobacco leaves which have been braided into the rods into a bulk curing barn; and curing the tobacco leaves which are loaded in a furnace, and obtaining the tobacco leaves with the lowTSNAs content through a yellowing stage, a color fixing stage and a stem drying stage. The special bacterial solution is inoculated to the fresh tobacco leaves which are about to be loaded in the furnace, and the stage changes of the environmental temperature and humidity in a drying chamber of the curing barn in the curing process are controlled according to the varieties of the tobacco leaves,so that the degradation of the TSNAs in the flue-cured tobaccos is promoted, the purpose of controlling the TSNAs content of the tobacco leaves to improve the quality of the flue-cured tobaccos is achieved, and the great significance is achieved for reducing the harm of the TSNAs in the tobaccos to the human health and the living environment.

Description

technical field [0001] The invention belongs to the technical field of tobacco curing, and in particular relates to a curing method for reducing TSNAs of different varieties of flue-cured tobacco by using bacterial strains, which can effectively reduce the TSNAs content of flue-cured tobacco to improve the quality of tobacco leaves. Background technique [0002] TSNAs are tobacco-specific N-nitrosamine-based compounds, and they are also one of the main harmful substances that affect human health. Among the 8 TSNAs identified so far, the 4 kinds of NNN, NAT, NAB and NNK are relatively high in content, and both NNN and NNK are strong carcinogens in animals. Confirmed in high incidence studies. Studies have shown that factors such as tobacco species, tobacco structure, tobacco cultivation, tobacco curing, soil microbial community, and fertilization amount are all related to the content of TSNAs in tobacco. In addition, a large number of studies have also shown that the conten...

AI Technical Summary

Problems solved by technology

Although predecessors have explored ways to reduce TSNAs in tobacco leaves from various aspects such as agricultural technology, modulation technology, and biotechnology, although the method of using bacteria to degrade TSNAs in the prior art is useful for directional cleaning of pollutants and purification of drinking water, etc. There are also bacterial liquids used in tobacco for drying and sedimentation of Burley tobacco, but it is rare to use bacterial strains to reduce TSNAs in tobacco leaves during the curing process, and there are few reports on methods for reducing TSNAs content in specific varieties of flue-cured tobacco

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