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A method for reducing tsnas in cigar tobacco leaves by using bacterial strains

A technology of cigars and tobacco leaves, which is applied in the field of tobacco industry, can solve the problems that the overall effect is not very obvious, and achieve the effects of improving survival rate and activity, reducing harm, and convenient operation

Active Publication Date: 2021-07-23
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the predecessors have explored ways to reduce nitrosamines (TSNAs) in tobacco leaves in many ways, most of which involve flue-cured tobacco, cigarettes, etc., including the use of equipment, tobacco leaf preparation, and improved technology, etc., but the overall effect is not very obvious. The method of degradation of cigar tobacco nitrosamines (TSNAs) is rarely reported, therefore, reducing cigar tobacco TSNAs by inoculating bacteria is a direction worthy of research

Method used

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  • A method for reducing tsnas in cigar tobacco leaves by using bacterial strains
  • A method for reducing tsnas in cigar tobacco leaves by using bacterial strains
  • A method for reducing tsnas in cigar tobacco leaves by using bacterial strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] S100: Harvest the upper leaves, middle leaves and lower leaves of tobacco plants with suitable maturity in Gengma County, Lincang City, Yunnan Province, wherein:

[0039] The lower leaves are light green or slightly yellow in green, the main veins are whitish, the branches are tender white, the leaves are smooth and shiny with green bands and yellowish, part of the glandular hairs has fallen off, and the tips of the leaves are slightly withered and yellow.

[0040] The middle leaves are collected in light green or yellowish green, with bright white main veins and light white branch veins. The leaf surface is slightly wrinkled and shiny greenish yellow, with mature spots, leaf tips and margins drooping, glandular hairs basically fall off, and the angle of stems and leaves increases. Large tobacco leaves.

[0041] The upper leaves are yellow-green or greenish-yellow, the main veins are white and bright, the branches are white, the leaves are wrinkled and shiny, yellow-gre...

Embodiment 2

[0052] On the basis of Example 1, 05-101, J45, J54 and mixed strains were inoculated during the drying process of cigars, and their effects on the degradation of nitrosamines (TSNAs) in cigars were determined.

[0053] Experiment 1 process:

[0054] Cultivate the 05-101, J45, and J54 strains obtained through the separation, screening and purification processes at 25-30°C for 60 hours to obtain a fermentation agent, and then mix the fermentation agent with sterilized water to obtain OD 600 Bacteria with a value of 1. In the air-curing process of cigars, 4% bacterial liquid by weight of tobacco leaves is sprayed evenly on the tobacco leaves in turn at the beginning of the air-curing yellowing period and the beginning of the color-fixing period for inoculation.

[0055] This experiment set up 4 treatments (TI, T2, T3, T4, CK), among which: the non-inoculated strain in Example 1 was used as the control (CK); the T1 bacterial solution was prepared with 05-101 strain; the T2 bacter...

Embodiment 3

[0059] On the basis of Example 1, 05-101, J45, J54 and mixed strains were inoculated during the fermentation of cigars, and their effects on the degradation of nitrosamines (TSNAs) in cigars were determined.

[0060] Experiment 2 process:

[0061] Cultivate the 05-101, J45, and J54 strains obtained through the separation, screening and purification processes at 25-30°C for 60 hours to obtain a fermentation agent, and then mix the fermentation agent with sterilized water to obtain OD 600 Bacteria with a value of 1. During the fermentation process of cigars, 4% bacterial liquid by weight of tobacco leaves was evenly sprayed on the tobacco leaves for inoculation at the beginning of fermentation and 21 days of fermentation respectively.

[0062] This experiment set up 4 treatments (TI, T2, T3, T4, CK), among which: the non-inoculated strain in Example 1 was used as the control (CK); the T1 bacterial solution was prepared with 05-101 strain; the T2 bacterial solution was prepared ...

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Abstract

The present invention discloses a method of using bacteria to reduce cigar tobacco leaves TSNAS, including fresh tobacco leaves with appropriate maturity; gradually drying the harvested fresh tobacco leaves in the drying house, and 05‑101 during the drying process, 05‑101, and 05‑101, during the drying process, 05‑101, and 05‑101, during the drying process, 05‑101, and 05‑101, during the drying process, 05‑101, and 05‑101, during the drying process, 05‑101, and 05‑101, and 05‑101.At least one type of bacteria prepared in J45, J54 600 Cycidiated liquid with a value of 1; the drying leaves after the drying of the tobacco stack are fermented. During the fermentation process, the 05‑101, J45, and J54 are made of at least one of the ODs prepared by the bacteria. 600 The bacteria liquid with a value of 1 can get the cigar tobacco leaves with low TSNAS.By selecting the three bacteria that can significantly reduce the cigar smoke, the single or mixed bacteria fermented bacterial solution is used to use the cigar tobacco drying and fermentation process., Achieving the efficient orientation of cigar tobacco, it has a high -efficiency and directional degradation of nitrosamine, and has good resistance, easy operation, and high promotion and application value.

Description

technical field [0001] The invention belongs to the technical field of the tobacco industry, and in particular relates to a method for reducing TSNAs of cigar tobacco leaves by using bacterial strains which is easy to operate, can efficiently and directionally degrade the TSNAs content of cigar tobacco, and has good stress resistance. Background technique [0002] Nitrosamines (TSNAs) are tobacco-specific N-nitrosamine-based compounds, which are harmful substances in tobacco and one of the main harmful substances that affect human health. Among the eight TSNAs identified so far, N-nitrosonornicotine (NNN), 4-(N-methyl-nitrosamine)-1-(3-pyridyl)-1-butane The four kinds of ketones (NNK), N-nitrosoanatabine (NAT) and N-nitrosoanabatin (NAB) were higher in content. As early as 1962, the potential carcinogenic effects of tobacco-specific nitrosamines (TSNAs) were reported for the first time in the world, leading to the formation of tumors in human lungs, mouth, esophagus, stomac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 胡彬彬胡梦阳邹聪明姜永雷朱艳梅夏振远陈颐蔺忠龙陈丹赵高坤何鲜苏家恩徐安传巩江世琪何元胜吴玉萍孔光辉晋艳李永平
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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