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A gold-curcumin nanoparticle quenching CDS hybrid tio 2 Electrochemiluminescent sensor for insulin detection with nanoribbons

A technology of nano-particles and curcumin, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve high-sensitivity detection, rapid detection, and improvement of water solubility

Active Publication Date: 2021-12-03
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently based on gold-curcumin nanoparticles to quench CdS@TiO 2 The method of detecting insulin has not been reported

Method used

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  • A gold-curcumin nanoparticle quenching CDS hybrid tio <sub>2</sub> Electrochemiluminescent sensor for insulin detection with nanoribbons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of CdS@TiO 2 chitosan solution

[0037] Disperse 0.1 g of cadmium acetate in 30 mL of ultrapure water, add 25 mg of titanium dioxide nanobelts, and sonicate for 1 h. Then, add 5 mL of 5 M Na 2 S solution, stirred for 12 h, transferred the above solution to a 100 mL reactor, reacted at 160 °C for 16 h, centrifuged, washed, and dried to obtain CdS@TiO 2 nanobelt.

[0038] The chitosan solution with a mass fraction of 0.5% was prepared by adding 0.5 g of chitosan to 100 mL of acetic acid with a volume fraction of 1%, and stirring for 2 h.

Embodiment 2

[0039] Example 2 Preparation of CdS@TiO 2 chitosan solution

[0040] Disperse 0.1 g of cadmium acetate in 30 mL of ultrapure water, add 50 mg of titanium dioxide nanobelts, and sonicate for 1 h. Then, add 5 mL of 5 M Na 2 S solution, stirred for 12 h, transferred the above solution to a 100 mL reactor, reacted at 160 °C for 16 h, centrifuged, washed, and dried to obtain CdS@TiO 2 nanobelt.

[0041] The chitosan solution with a mass fraction of 0.5% was prepared by adding 0.5 g of chitosan to 100 mL of acetic acid with a volume fraction of 1%, and stirring for 2 h.

Embodiment 3

[0042] Example 3 Preparation of Secondary Antibody-Gold-Curcumin / ZIF-8 Solution

[0043] (1) Preparation of gold-curcumin nanoparticles

[0044] First, 8 mg of curcumin was dispersed into 5 mL of dimethyl sulfoxide to form a transparent and uniform solution. Then, 2 mL of curcumin-dimethyl sulfoxide solution was added to 45 mL of ultrapure water, and stirred under reflux at 100 °C. Add 100 μL 0.1 M NaOH, when the solution is heated to 100 °C, add 1 mL 2% HAuCl 4 The solution was stirred for 1 h, cooled to room temperature, and dialyzed with a 12 kDa dialysis bag to remove unreacted impurities and small particles to obtain a gold-curcumin solution.

[0045] (2) Preparation of gold-curcumin / ZIF-8 nanoflowers

[0046] Mix 1 mL of 2-methylimidazole with 2 mL of gold-curcumin solution and stir for 10 min. Afterwards, 1 mL of 20 mM zinc acetate solution and 0.05 g of bovine serum albumin were added, and stirred for 4 h. Finally, the obtained gold-curcumin / ZIF-8 nanoflowers were...

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Abstract

The invention relates to a gold-curcumin nanoparticle quenching CdS hybrid TiO 2 Nanoribbon-based electrochemiluminescence sensor for insulin detection. In the present invention, potassium persulfate and hydrogen peroxide are used together as co-reactants to enhance the CdS hybrid TiO 2 Electrochemiluminescence properties of nanoribbons. In order to detect insulin sensitively, the present invention designs a sandwich-type quenching electrochemiluminescence immunosensor, which uses gold-curcumin complex ZIF-8 as a quencher to reduce the CdS hybrid TiO 2 Electrochemiluminescent intensity of the nanoribbons. The quenching mechanism is mainly due to the fact that curcumin consumes hydroxyl radicals generated during the electrochemical reaction, and further reduces the luminous intensity of the luminescent material through energy transfer, thereby realizing double quenching of the electrochemiluminescent signal. According to different concentrations of insulin, different amounts of secondary antibody marker gold-curcumin complex ZIF-8 can be combined, so that the sensor's electrochemiluminescence intensity changes differently. The linear range of the present invention for insulin detection is 0.3 pg / mL-20 ng / mL, and the detection limit is 0.09 pg / mL.

Description

technical field [0001] The invention relates to a gold-curcumin nanoparticle quenching CdS hybrid TiO 2 Nanoribbon-based electrochemiluminescence sensor for insulin detection. Specifically, CdS hybrid TiO 2 Nanobelts are used as luminescent materials, and gold-curcumin nanoparticles are used as quenchers. In order to enhance the quenching effect, gold-curcumin nanoparticles are combined with ZIF-8 (gold-curcumin / ZIF-8) to prepare a detection insulin The invention discloses a quenching type electrochemiluminescence sensor, which belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Insulin is a polypeptide hormone that regulates fat and carbohydrate metabolism. Clinically, the detection of insulin is very meaningful, because the content of insulin is an important indicator for evaluating endocrine function, and provides a basis for detecting diabetes, insulinoma, insulin resistance syndrome, etc. Therefore, in the present inve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 杜宇李小建任祥孙晓君魏琴杨兴龙王雪莹吴丹马洪敏王欢
Owner UNIV OF JINAN
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