Single spore separation device, single spore separation microscope and single spore separation method

Abstract

The invention discloses a single spore separation device, a single spore separation microscope and a single spore separation method. The single spore separation device of the present invention comprises a lifting regulator, a connecting rod, a needle-shaped object fastener and a separation needle; the lifting regulator is installed at the position of the condenser lifting regulator on the microscope mirror arm; Control the rise or fall of the lifting regulator; one end of the connecting rod is connected to the lifting regulator, and the other end of the connecting rod is connected to the needle-shaped object fastener; one end of the separation needle is locked Inside the pin object fastener. The invention has the advantages of ensuring the accuracy of single spore separation; the height of the separation needle can be adjusted; the separation needle is not easy to break; frequent focusing is not required; and the operation process is simple.

Description

technical field [0001] The invention belongs to the technical field of optical microscopes. Background technique [0002] Fungal single spore isolation technology is one of the most basic key technologies in the process of fungal research. Fungal disease identification, pathogenicity analysis, fungal system evolution and genetic variation analysis all require that the pathogen is a pure strain derived from one spore, and single spore isolation is required. [0003] So far, there have been many research reports on fungal single spore isolation technology. Such as the early dilution separation method, agar plate digging method, glass capillary separation method, and later fixed insect needle picking method, eyebrow needle picking method, solid mirror picking method, etc., and later improved methods based on these, such as Picking needle picking method (under a microscope, use a self-made picking needle to pick single spores), medium picking method (under a microscope, look f...

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Problems solved by technology

[0009] 2. Single spore filaments are easy to break

Since the top of the single-spore separation filaments is always in contact with the bottom of the medium, but the surface of the medium is not a uniform level, the slightly raised medium forms resistance during the movement of the stage, and the fragile single-spore separation filaments are easy to to be broken

[0010] 3. The requirements for single spore isolated silk are high, and only skilled technicians can produce single spore isolated silk that meets the requirements

Since the length and angle of the single spore separation filaments of the single spore separation microscope cannot be adjusted, the single spore separation filaments are longer or shorter, or the angle of the single spore separation filaments deviates a little, which will lead to the inability to clearly see the single spore separation filaments under the magnified field of view of the microscope. The needle point of the spore separation filament, or the needle point cannot be clearly visualized at the same time as the spores on the medium

Therefore, it is difficult for beginners or ordinary operators to produce monospore isolated filaments with just the right length and angle.

[0011] 4. The thickness of the single-spore separation silk cannot be adjusted: the single-spore separation silk is fixed on the microscope base, and its aperture is fixed, so that only one specification of single-spore separation silk can be placed, and the single-spore separation silk that is too thick or too thin cannot be placed

However, the spore size of different fungi is quite different, and the large spores cannot be picked with fine single-spore separation filaments, while the small spores are easy to cause pollution when picked with thick separation filaments. In actual operation, it is necessary to use different specifications according to the size of the spores. Monospore septa work

[0012] 5. Requires frequent focusing and low efficiency

Frequent focusing can easily cause operator eye fatigue, thereby reducing work efficiency

[0013] 6. The operation is cumbersome, and redundant objective lenses need to be disassembled back and forth

Because the length of single spore separation filaments cannot be adjusted in the existing single spore separation microscope, during the process of picking single spores, it is necessary to remove the redundant objective lens, otherwise the petri dish cannot be placed on it, and the single spore separation filaments will be broken if placed forcibly

In this way, in order to restore the function of the general microscope after the separation of single spores, other objective lenses need to be installed, and the operation is cumbersome due to back and forth disassembly and assembly.

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