Single spore separation device, single spore separation microscope and single spore separation method

A single spore separation and microscopy technology, applied in the field of optical microscopy, can solve the problems of frequent focusing, low efficiency, cumbersome operation, etc., and achieve the effect of avoiding misoperation, not easy to break, and ensuring accuracy

A single spore separation and microscopy technology, applied in the field of optical microscopy, can solve the problems of frequent focusing, low efficiency, cumbersome operation, etc., and achieve the effect of avoiding misoperation, not easy to break, and ensuring accuracy

CN110923121BActive Publication Date: 2021-07-02HUAZHONG AGRI UNIV
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  • Single spore separation device, single spore separation microscope and single spore separation method
  • Single spore separation device, single spore separation microscope and single spore separation method
  • Single spore separation device, single spore separation microscope and single spore separation method

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Effect test

Embodiment approach

[0126] A kind of embodiment of single spore isolation method of the present invention comprises the following steps:

[0127] (1) Prepare a petri dish with transparent medium laid on the bottom, and smear the conidia of the bacterial strain to be isolated on the surface of the medium;

[0128] (2) The culture dish is fixed on the microscope stage 9, so that the medium surface faces the separation needle 8;

[0129] (3) Rotate the vertical regulator and / or the horizontal regulator of the microscope stage 9 to find the target single spore under the microscope field of view;

[0130] (4) Utilize the lifting hand wheel 10 to control the lifting regulator 4, and / or adjust the needle-shaped object fastener 5, so that the separation needle 8 is close to the target single spore;

[0131] (5) Utilize the lifting hand wheel 10 to control the lifting regulator 4 to make the separation needle 8 contact the culture medium;

[0132] (6) Adjust the longitudinal regulator and / or the horizon...

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Abstract

The invention discloses a single spore separation device, a single spore separation microscope and a single spore separation method. The single spore separation device of the present invention comprises a lifting regulator, a connecting rod, a needle-shaped object fastener and a separation needle; the lifting regulator is installed at the position of the condenser lifting regulator on the microscope mirror arm; Control the rise or fall of the lifting regulator; one end of the connecting rod is connected to the lifting regulator, and the other end of the connecting rod is connected to the needle-shaped object fastener; one end of the separation needle is locked Inside the pin object fastener. The invention has the advantages of ensuring the accuracy of single spore separation; the height of the separation needle can be adjusted; the separation needle is not easy to break; frequent focusing is not required; and the operation process is simple.

Description

technical field [0001] The invention belongs to the technical field of optical microscopes. Background technique [0002] Fungal single spore isolation technology is one of the most basic key technologies in the process of fungal research. Fungal disease identification, pathogenicity analysis, fungal system evolution and genetic variation analysis all require that the pathogen is a pure strain derived from one spore, and single spore isolation is required. [0003] So far, there have been many research reports on fungal single spore isolation technology. Such as the early dilution separation method, agar plate digging method, glass capillary separation method, and later fixed insect needle picking method, eyebrow needle picking method, solid mirror picking method, etc., and later improved methods based on these, such as Picking needle picking method (under a microscope, use a self-made picking needle to pick single spores), medium picking method (under a microscope, look f...

Claims

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Application Information

Patent Timeline
02 Jul 2021
Publication
CN110923121B
IPC
C12M1/00; C12N3/00; G02B21/34
CPC
C12M23/52; C12M33/00; C12N3/00; G02B21/34
Inventors
尹良芬; 罗朝喜