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Mycoplasma freeze-drying protective agent, freeze-dried mycoplasma and preparation method thereof
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A freeze-dried protective agent and mycoplasma technology, applied in the biological field, can solve the problems of unsatisfactory freeze-dried protective agent effect, low viable bacteria rate of freeze-dried mycoplasma, short storage period, etc., so as to reduce the death of mycoplasma and prolong the freeze-dried storage time. , the effect of protecting mycoplasma
Active Publication Date: 2021-07-20
天康制药股份有限公司
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[0005] The first object of the present invention is to provide a mycoplasma freeze-drying protectant and its preparation method, so as to alleviate the problems in the prior art that the effect of the mycoplasma freeze-drying protectant is not ideal, the rate of freeze-dried mycoplasma viable bacteria is low, and the storage period is short.
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[0031] A kind of preparation method of lyoprotectant, the dextran of prescription amount, sorbitol, hydrolyzed milk protein, transferrin, gelatin, trehalose, skimmed milk, Na 2 HPO 4 .12H 2 O and NaH 2 PO 4 .2H 2 O, mixed with water to obtain a lyoprotectant. The preparation of the lyoprotectant provided by the present invention has no special requirements, and it only needs to mix each component in water according to the ratio of raw materials, which is low in cost and easy to operate.
[0032] In a preferred embodiment, after mixing with water, the lyoprotectant is sterilized to obtain the lyoprotectant. Preferably, the sterility is sterilized by filtration with a 0.22 micron filter membrane. Sterilization by filtration can effectively sterilize, avoid contamination of mycoplasma strains, and at the same time avoid damage to the hydrolyzed milk protein in the components, which will affect the effect of the freeze-drying protective agent.
[0033] The invention relates ...
Embodiment 1
[0038] A lyoprotectant for mycoplasma, comprising: 1w / v% dextran, 4w / v% sorbitol, 4w / v% hydrolyzed milk protein, 2w / v% transferrin, 0.5w / v% gelatin, 5w / v % trehalose, 1w / v% skimmed milk, 1.5w / v% Na 2 HPO 4 .12H 2 O and 0.05w / v% NaH 2 PO 4 .2H 2 O, the balance is water.
Embodiment 2
[0040] A lyoprotectant for mycoplasma, comprising: 3w / v% dextran, 2w / v% sorbitol, 6w / v% hydrolyzed milk protein, 0.5w / v% transferrin, 3w / v% gelatin, 1w / v % trehalose, 3w / v% skimmed milk, 0.5w / v% Na 2 HPO 4 .12H 2 O and 0.15w / v% NaH 2 PO 4 .2H 2 O, the balance is water.
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Abstract
The invention relates to the field of biotechnology, and specifically provides a mycoplasma freeze-drying protectant, freeze-dried mycoplasma and a preparation method thereof. Lyoprotectant solution includes 1‑3w / v% dextran, 2‑4w / v% sorbitol, 4‑6w / v% hydrolyzed milk protein, 0.5‑2w / v% transferrin, 0.5‑3w / v% gelatin, 1‑5w / v% trehalose, 1‑3w / v% skimmed milk, 0.5‑1.5w / v% Na 2 HPO 4 .12H 2 O and 0.05‑0.15w / v% NaH 2 PO 4 .2H 2 O, the balance is water. The freeze-drying protection solution is designed for the freeze-drying preservation of mycoplasma strains, and the synergistic effect between the components can significantly reduce the death of mycoplasma during the freeze-drying process and storage period, effectively protect the mycoplasma, and prolong the freeze-drying storage time of the mycoplasma.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a mycoplasma freeze-drying protectant, freeze-dried mycoplasma and a preparation method thereof. Background technique [0002] Mycoplasma (mycoplasma) is a kind of smallest prokaryotic cell-type microorganisms without cell walls, highly pleomorphic, able to pass through bacteria filters, and can be cultured and proliferated in artificial medium, with a size of 0.1-0.3 microns. Because it can form filamentous and branched shapes, it is called mycoplasma. Mycoplasma widely exists in humans and animals, most of which are not pathogenic, but there are also a few pathogenic mycoplasma, such as Mycoplasma hyopneumoniae, Mycoplasma genitalium, Mycoplasma bovis and so on. Mycoplasma bovis is an important pathogenic pathogen that can cause various diseases such as pneumonia, arthritis, mastitis, keratoconjunctivitis, otitis, reproductive tract inflammation, even abortion and infertility in c...
Claims
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Application Information
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