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Bifidobacterium animal subspecies lactis JMCC0025 and isolation and purification method and application of JMCC0025

A technology for separation and purification of bifidobacteria in animals, which is applied in the field of bioengineering and can solve problems such as dependence and loss of the ability to reproduce probiotics in the intestines

Inactive Publication Date: 2020-03-27
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, medical research has shown that if artificially synthesized probiotics are used for a long time, the intestinal tract may gradually lose its ability to reproduce probiotics, making the human intestinal tract dependent on artificial probiotics, resulting in "probiotic dependence"

Method used

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  • Bifidobacterium animal subspecies lactis JMCC0025 and isolation and purification method and application of JMCC0025
  • Bifidobacterium animal subspecies lactis JMCC0025 and isolation and purification method and application of JMCC0025
  • Bifidobacterium animal subspecies lactis JMCC0025 and isolation and purification method and application of JMCC0025

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 A Bifidobacterium lactis subspecies JMCC0025

[0049] This example provides an animal Bifidobacterium lactis subsp. JMCC0025, which is isolated and screened from the feces of breastfed infants or young children. General Microbiology Center, the preservation address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18403, and the Latin name is Bifidobacterium animalis subsp lactis .

[0050] The 16SrRNA sequence of the above-mentioned Bifidobacterium animalis subsp. lactis JMCC0025 strain is as follows:

[0051] ACGGCTCCCCCACAAGGGTCGGGCCACCGGCTTCGGGTGCTACCCACTTTCATGACTTGACGGGCGGTGTGTACAAGGCCCGGGAACGCATTCACCGCGGCGTTGCTGATCCGCGATTACTAGCGACTCCGCCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGACCGGTTTTCAGCGATCCGCCCCACGTCACCGTGTCGCACCGCGTTGTACCGGCCATTGTAGCATGCGTGAAGCCCTGGACGTAAGGGGCATGATGATCTGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCGGTCCCACATGAGTTCCCGGCATCACCCGCTGGCAACATGCGGCGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACAC...

Embodiment 2

[0054] Example 2 A method for separation and purification of Bifidobacterium lactis subspecies JMCC0025

[0055] This embodiment provides the separation and purification method of embodiment 1, which is carried out according to the following sequence of steps:

[0056] 1. Collect samples

[0057] Take 1g of intestinal feces from infants or young children, then add it to 9ml of normal saline and mix well to obtain sample A;

[0058] 2. Sample enrichment

[0059] Take V 1 = 2ml sample A, added to V 2 =100mL modified MRS liquid medium, at T 1 = 37 ℃ anaerobic culture t 1 =72h, obtain culture medium B;

[0060] 3. Isolation and screening of strains

[0061] Take 1ml of culture medium B1 and dilute it with 0.9% sterile saline in a 10-fold gradient, followed by a 10-fold gradient dilution. -1 、10 -2 、10 -3 、10 -4 、10 -5 times, corresponding to the bacterial suspension C1 1 ~C1 5 ;

[0062] Take the improved MRS solid medium, after melting, pour it into the first to fi...

Embodiment 3-6

[0071] Example 3-6 A separation and purification method of Bifidobacterium lactis JMCC0025

[0072] Embodiment 3-6 is respectively the separation and purification method of embodiment 1, is basically the same as the method of embodiment 2, and difference is that the technical parameter of separation and purification process is different, and specific parameters are as shown in table 1:

[0073] Table 1 Example 3-6 Separation and purification process and parameters

[0074]

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Abstract

The invention discloses an Bifidobacterium animal subspecies lactis JMCC0025. The strain of JMCC0025 was deposited in General Microbiology Center of China Microbiological Culture Collection ManagementCommittee on August 20, 2019, the deposit address is No. 3, yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO. 18403; and the invention also discloses a methodof isolation and purification and application of the above Bifidobacterium animal subspecies lactis JMCC0025. The Bifidobacterium animal subspecies lactis JMCC0025 provided by the present invention can regulate intestinal flora balance, improve fecal characteristics, and has better better survival rate of simulated digestive fluid; and under the storage condition of 37 DEG C, which is suitable for the growth of lactic acid bacteria, the post-acid can be well controlled. The invention belongs to the technical field of bioengineering, and the provided Bifidobacterium animal subspecies lactis JMCC0025 has the function of regulating intestinal flora balance, and can be used in the process of making food, medicine or beverage.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a bacterial strain, a screening method and an application, specifically, animal Bifidobacterium lactis subspecies JMCC0025, a separation and purification method and application thereof. Background technique [0002] Bifidobacterium was first discovered by Henry Tissier in 1899 when it was isolated from the feces of breastfed infants. Bifidobacterium is a Gram-positive bacillus, which is very sensitive to oxygen, has poor tolerance to low pH, and is easily inactivated in a low pH environment; its optimum pH is 6.5-7.0, and its optimum growth temperature is 37-42°C. Bifidobacteria widely exist in the digestive tract, vagina and oral cavity of humans and animals, and are one of the important members of the intestinal flora of humans and animals. It has been found that there are 32 subtypes of Bifidobacteria, and there are as many as 70 biological agents containing Bifidobacter...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A23L2/38A23C9/123A23L7/104A23L13/40A23L13/70A23L33/135A61K35/745A61P1/00C12R1/01
CPCC12N1/20C12N1/02A23L2/382A23C9/1234A23L7/104A23L13/45A23L13/74A23L33/135A61K35/745A61P1/00A23V2002/00C12R2001/01C12N1/205A23V2400/515A23V2200/3204Y02A40/90A23L13/30A23V2200/3202
Inventor 冯丽莉张栋朱宏王世杰荀一萍薛玉玲康志远韩晓伟
Owner JUNLEBAO DAIRY GRP CO LTD
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