Modules for transferring magnetic beads, automated system comprising same and method for nucleic acid extraction using same
An automated system and nucleic acid extraction technology, applied in the field of new modules, to achieve the effect of reducing the use of reagents and low cost
Pending Publication Date: 2020-03-31
SEEGENE INC
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[0009] The methods for extracting nucleic acids disclosed in Japanese Patent No. 3794734 and U.S. Patent No. 7,329,488 are carried out in equipment specially designed for bead-transfer-only methods, and thus still suffer from the above-mentioned disadvantages of bead-transfer-type methods
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[0086] Hereinafter, aspects of the exemplary embodiments will be described in detail with reference to the accompanying drawings. Reference numerals are added to elements in each drawing, and where possible, the same elements will be designated by the same reference numerals even though they are shown in different drawings. Also, in the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention unclear.
[0087] In addition, terms such as first, second, (a), (b), (i), (ii), etc. may be used herein when describing components or steps of the present invention. These terms are only used to distinguish one element from other elements of the present invention, and the characteristics, order, sequence, etc. of the corresponding elements are not limited by the terms.
[0088] The phrase "at least one of A and B" or "A and / or B" means A, B...
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Abstract
The present invention relates to novel modules for transferring magnetic beads, an automated system comprising the same and a method for extracting nucleic acids using the same. The specifically designed magnet module and cover module of the present invention can be employed in the automated liquid handling apparatus by means of pre-existing moving modules (e.g., pipettor module) of the apparatus.The present invention enables a bead transfer-type method for extracting nucleic acids to be performed in an automated manner on the automated liquid handling apparatus. The present invention provides advantages of higher level of automation, more reduced cost and no need for another separate liquid handling apparatus compared to the conventional bead transfer-type method usually performed in thesmall apparatus designed to be used only for the bead transfer-type method. Also, the present method has the merits of more shortened reaction time compared to the conventional liquid transfer-type method.
Description
technical field [0001] The present invention relates to a novel module for transferring magnetic beads, an automated system comprising the module and a method for extracting nucleic acid using the module. Background technique [0002] A nucleic acid amplification technique capable of amplifying a specific gene of interest has been used in various fields such as in vitro diagnostics for determining pathogen infection in humans, animals, and plants, genotyping, and genetic analysis in GMO food testing. In order to efficiently amplify specific genes, nucleic acids in various samples should be extracted and purified by removing any substances that inhibit nucleic acid amplification. [0003] Traditionally, for small-scale experiments, nucleic acid extraction has been performed manually. However, due to the increase in the number of samples from which nucleic acid must be extracted at one time and the necessity of quality control of the extraction process, many devices for autom...
Claims
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IPC IPC(8): G01N35/00G01N35/10C12N15/10C12Q1/6806
CPCB03C1/01B03C1/0332B03C1/284B03C1/286B03C2201/18B03C2201/26B03C2201/28C12N15/1013C12Q1/6806C12M47/06G01N35/0098G01N2035/00534G01N2035/00564C12Q2563/143C12Q2563/149C12Q2565/629C12Q2531/113G01N35/1065G01N2035/1053C12M23/06C12M23/38G01N35/10
Inventor 李承宰洪性植金英旭
Owner SEEGENE INC