Biological bactericide for crude oil transportation pipelines, and applications thereof
A biological fungicide and transportation pipeline technology, applied in the field of environmental engineering, can solve the problems of difficult coating, unenvironmental protection and high cost, and achieve the effects of inhibiting microbial corrosion, improving corrosion resistance and avoiding drug resistance.
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Embodiment 1
[0033] The preparation of embodiment 1 biocides
[0034] Take the alkanophagous bacteria stored in 2216E liquid medium containing 20% glycerol at -80°C, thaw at 25°C, inoculate 1000 μl in 2216E liquid medium, and shake at 37°C at 120r / min Cultivate for 24 hours, obtain the bacterial liquid, and dilute the bacterial liquid to 1×10 8 CFU / ml, the biocide is obtained.
Embodiment 2
[0035] The inhibitory effect of embodiment 2 biological bactericides to corrosion bacteria
[0036] Get the biological fungicide prepared by 0.001kg embodiment 1 and dilute 500-1000 times and join in the postgate substratum, then add sulfate-reducing bacteria (SRB), the addition of sulfate-reducing bacteria (SRB) and the addition of fungicide Same, its ratio is 1:1. In this embodiment, an experimental group and a control group are set up. The experimental group contains both fungicides (Alkanophagous boku) and SRB, and the control group contains only SRB.
Embodiment 3
[0037] Two kinds of bacteria relative content and pH measurement in the embodiment 3 system
[0038] Take 50ml samples of the experimental group and control group in Example 2 on 1 day, 3 days, 5 days, 7 days, 9 days, 11 days, and 14 days respectively, and send them to Meiji Biological Company for biodiversity detection, and measure The pH values of the two groups.
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Abstract
Description
Claims
Application Information
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