Preparation method for producing digestion-free protein from animal proteins

A technology of animal protein and protein, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as protein imbalance, insufficient cell protein recycling, long-term body protein deficiency, etc.

Pending Publication Date: 2020-04-17
CHANGCHUN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] It is to solve the problem of long-term body protein deficiency caused by insufficient recycling of cell protein in the body due to the aging of the body of the elderly, replace the old product of protein powder with biotechnology, provide new products of more valuable biotechnol...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Weigh 100kg of fish meat.

[0024] 2. Cut fish meat protein raw materials into pieces, crush them, and mix them with 9-10 times of water.

[0025] 3. Ultra-high temperature sterilization, aging and protein denaturation treatment are carried out on the feed liquid after mixing, and the temperature is kept at 100°C for more than 15 minutes.

[0026] 4. Perform cooling treatment to reduce the temperature of the feed liquid to between 54°C and 56°C.

[0027] 5. Add 0.3%-0.5% compound protease by weight of raw materials for enzymolysis treatment. In this example, add 0.3kg compound protease and enzymolysis time is 70 hours. There is no need to adjust the pH value, but slow stirring is required.

[0028] 6. The enzymatic solution is separated by membrane to remove the unavoidably digestible protein larger than 500 Da. After purification, the enzymatic solution is treated at 90°C for 30 seconds to obtain high-purity non-digestible protein products.

[0029] 7. Treat the ...

example 2

[0032] 1. Weigh 200kg of beef.

[0033] 2. Cut beef raw materials into pieces, crush them, and mix them with 9-10 times of water.

[0034] 3. Ultra-high temperature sterilization, aging and protein denaturation treatment are carried out on the feed liquid after mixing, and the temperature is kept at least 100°C for more than 15 minutes.

[0035] 4. Carry out cooling treatment, so that the temperature of the feed liquid is between 15°C and 40°C. (Because the fat content of beef is 4.2%), the fat needs to be hydrolyzed into fatty acids, diglycerides, monoglycerides and glycerol. Add 0.2%-0.5% lipase, experiment 2 added 25 grams of lipase. After 6 hours of treatment, the temperature of the feed solution was raised to between 54°C and 56°C for proteolysis.

[0036] 5. Add 0.3%-0.5% compound protease by weight of raw materials for enzymolysis treatment. Experiment 2. Add 0.6 kg compound protease, and the enzymolysis time is 70 hours. The enzymolysis process does not need to adjus...

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PUM

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Abstract

The invention discloses a preparation method for producing a digestion-free protein from animal proteins (mainly referring to lean meat and fish meat of animals). The digestion-free protein is a smallmolecular protein with the molecular weight of 500 Da (Dalton) or below, and comprises compound amino acid with the average molecular weight of 120 Da and small molecular dipeptide and tripeptide with the average molecular weight of 180-500 Da. The protein does not need to be digested by a human body, and can directly enter the body through small intestines of the human body and be absorbed and utilized. The quality of the product in the domestic peptide industry is determined by the content of dipeptide and tripeptide, due to peptides with the content larger than 500 Da are not directly absorbed and utilized by the human body, and products obtained through the technology are basically not digested secondarily after being eaten by the human body. A membrane separation technology can be adopted on a production line to produce the high-purity digestion-free protein product, and the digestion-free protein product containing a small amount of more than 500 Da can also be produced withoutadopting a purification process.

Description

technical field [0001] The invention relates to a new food with protease deep processing technology. Specifically, it converts macromolecular soybean protein into a non-digestible protein product with a molecular weight below 500 Da and can be directly absorbed by the small intestine of a human body through the shearing action of an enzyme. Its components are compound amino acids with an average molecular weight of 120Da and small molecule dipeptides and tripeptides with an average molecular weight of 180Da to 500Da. This section of protein does not need to be digested by the human body, and can directly enter the body through the small intestine of the human body to be absorbed and utilized. The quality of products in the domestic peptide industry is determined by the content of dipeptides and tripeptides. The reason is that peptides larger than 500 Da are not directly absorbed and utilized by the human body, and products of this type of technology have a protective film that...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 朱振伟朱光
Owner CHANGCHUN UNIV
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