Supercharge Your Innovation With Domain-Expert AI Agents!

A novel nucleic acid aptamer electrochemical sensor for the detection of sialic acid based on a dual signal amplification strategy

A nucleic acid aptamer and sialic acid technology, which is applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve the effects of large surface area, high repeatability and stability, and improved sensitivity

Active Publication Date: 2022-03-11
SHANGQIU NORMAL UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no related report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A novel nucleic acid aptamer electrochemical sensor for the detection of sialic acid based on a dual signal amplification strategy
  • A novel nucleic acid aptamer electrochemical sensor for the detection of sialic acid based on a dual signal amplification strategy
  • A novel nucleic acid aptamer electrochemical sensor for the detection of sialic acid based on a dual signal amplification strategy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation method of nucleic acid aptamer electrochemical sensor for detection of sialic acid based on dual signal amplification strategy

[0030] (1) Deposition of gold nanoleaf on carbon cloth electrode: Before electrode modification, the carbon cloth electrode (1.0×1.5 cm) was ultrasonically cleaned with ultrapure water, ethanol and ultrapure water in an ultrasonic cleaner for 7 min, Dip the treated carbon cloth electrode into 10mL40mmol·L -1 Electrodeposition was carried out at a constant potential of -0.08V for 400s in the chloroauric acid solution to obtain the carbon cloth surface with gold nanoleaf structure.

[0031] (2) Immobilization of the nucleic acid aptamer and recognition of sialic acid: the sequence of the selected nucleic acid aptamer is TCCCTACGGC GCTAA CCGAT AGGTG TAGCG TGGGG CACAT GTTCG CGCCA CCGTG CTACA AC, and the gold nanoleaf prepared in step (1) was modified Immerse the carbon cloth electrode into 40 μL of 80 μmol L -1 Incubate in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a nucleic acid aptamer electrochemical sensor for detecting sialic acid based on a dual signal amplification strategy, and belongs to the technical field of novel functional nanocomposite materials and biosensor detection. The invention adopts high-conductivity gold nano-leaf modified carbon cloth as the base electrode, and performs signal amplification once. Further use of nucleic acid aptamers to achieve highly specific recognition of sialic acid improves the selectivity of the assay. The thionine / gold nanoparticles / 4-mercaptophenylboronic acid composite material is combined through the reaction of boronic acid-diol to introduce the electrochemical signal of thionine and realize the secondary signal amplification of the signal. The nucleic acid aptamer electrochemical sensor prepared by the method exhibits high sensitivity, good selectivity and long-term stability in the determination of sialic acid. The sensor is used for the determination of sialic acid in real serum samples, and the detection limit can reach 60 nmol L ‑1 .

Description

technical field [0001] The invention relates to the technical field of biosensor detection and functional nanocomposite materials, in particular to a nucleic acid aptamer electrochemical sensor based on double signal amplification of gold nanoleaf modified carbon cloth and functionalized gold nanoparticles, its preparation method and its application in saliva Acid detection applications. Background technique [0002] Sialic acid is an acetylated derivative of neuraminic acid, located at the end of the sugar chain of gangliosides. It widely exists in biological tissues and body fluids, and participates in various physiological processes such as cell recognition, adhesion and contact inhibition. For patients with malignant tumors, due to significant changes in the structure and content of glycoproteins and glycolipids on the surface of tumor cells, a large amount of sialic acid may fall off from the surface of cancer cells and enter the blood, which will increase the level of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/3278G01N27/3277G01N27/308
Inventor 周艳丽吕玉冰董辉刘澜涛徐茂田
Owner SHANGQIU NORMAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com