Method for preparing morinda citrifolia polysaccharide with high anti-cancer and antioxidant activity by cascade physical field

A technology of antioxidant activity and physical field, applied in the direction of anti-toxic agents, drug combinations, anti-tumor drugs, etc., can solve the problems of low molecular weight polysaccharides, low yield and low purity of noni polysaccharides, and achieve rapid and efficient preparation, Antioxidant performance and anticancer ability are improved, and the effect of anticancer activity is good

Active Publication Date: 2020-05-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is no relevant research on the preparation of low molecular weight polysaccharides by cascade physical field.
[0005] At present, the extraction of noni polysaccharides mainly adopts the traditional hydrothermal extraction method, which has high energy consumption, long time and low yield of noni polysaccharides, low purity and poor activity.

Method used

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  • Method for preparing morinda citrifolia polysaccharide with high anti-cancer and antioxidant activity by cascade physical field
  • Method for preparing morinda citrifolia polysaccharide with high anti-cancer and antioxidant activity by cascade physical field
  • Method for preparing morinda citrifolia polysaccharide with high anti-cancer and antioxidant activity by cascade physical field

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Such as figure 1 As shown, a method for preparing noni polysaccharides with high anticancer and antioxidative activity in a cascade physical field comprises the following steps:

[0035] Noni fruit raw material processing: dry Noni fruit (Noni fruit is a mature Noni fruit in the white ripe stage) at 50°C, powder, sieve (over 80 mesh sieve), add 4 times the volume percentage of 80% ethanol by volume The solution was refluxed for 4 hours to degrease and decolorize, and then dried to obtain noni fruit powder.

[0036] Cascade physical field-assisted extraction of noni polysaccharides: take 35g of noni fruit powder according to the ratio of material to liquid 1:35 (the mass of noni fruit powder and distilled water in the ratio of material to liquid are in grams and milliliters respectively) and add distilled water into the intermittent pulse electric field extraction equipment The medium was treated 30 times with a pulse frequency of 1 Hz and a treatment field strength of ...

Embodiment 2

[0073] Such as figure 1 As shown, a method for preparing noni polysaccharides with high anticancer and antioxidative activity in a cascade physical field comprises the following steps:

[0074] Noni fruit raw material processing: dry the Noni fruit (the Noni fruit is the mature Noni fruit of the white ripe stage) at 48°C, dry and beat the powder and sieve (over 80 mesh sieve), add 4 times the volume percentage of 85% ethanol solution by volume Reflux treatment for 4 hours to degrease and decolorize, then dry to obtain noni fruit powder.

[0075] Cascade physical field-assisted extraction of noni polysaccharides: Take 30g of noni fruit powder and add distilled water according to the ratio of material to liquid 1:30, and put it into the intermittent pulse electric field extraction equipment for 35 times, the pulse frequency is 5Hz, and the treatment field strength is 5kV / cm, then Ultrasonic equipment was used for ultrasonic treatment for 28 minutes, the ultrasonic power was 600...

Embodiment 3

[0093] Such as figure 1 As shown, a method for preparing noni polysaccharides with high anticancer and antioxidative activity in a cascade physical field comprises the following steps:

[0094] Noni fruit raw material processing: Noni fruit (noni fruit is a ripe noni fruit in the white ripe stage) is dried at 45°C, powdered and sieved (through a 100-mesh sieve), and 4 times of 75% ethanol solution is added by volume to reflux for 4 hours After degreasing and decolorizing, dry the noni fruit powder.

[0095] Cascade physical field-assisted extraction of noni polysaccharides: Take 25g of noni fruit powder and add distilled water according to the ratio of material to liquid 1:25, and put it into the intermittent pulse electric field extraction equipment for 40 times, the pulse frequency is 4Hz, and the treatment field strength is 6kV / cm, then Ultrasonic equipment was used for ultrasonic treatment for 20 minutes, the ultrasonic power was 600W, the ultrasonic frequency was 40kHz, ...

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Abstract

The invention discloses a method for preparing morinda citrifolia polysaccharide with high anti-cancer and antioxidant activity by a cascade physical field. The method comprises the following steps: (1) drying and powdering morinda citrifolia, degreasing and decoloring with ethanol, and drying to obtain morinda citrifolia powder; (2) preparing a polysaccharide extraction solution from the morindacitrifolia powder by adopting an intermittent pulsed electric field-ultrasonic wave-continuous pulsed electric field treatment mode, and (3) carrying out purification, low-temperature concentration, alcohol precipitation separation and freeze drying on the extraction solution to obtain morinda citrifolia polysaccharide. The method is high in polysaccharide yield and low in energy consumption, acidor enzyme is not needed, and prepared morinda citrifolia polysaccharide has a relatively small molecular weight, relatively high uronic acid content and relatively good anti-cancer and antioxidant activity. Meanwhile, morinda citrifolia polysaccharide has relatively high chelating capability with zinc, and the anti-cancer activity of the formed morinda citrifolia polysaccharide-zinc chelate is greatly increased.

Description

technical field [0001] The present invention relates to noni polysaccharides, in particular to a method for preparing noni polysaccharides with high anti-cancer and antioxidant activity in a cascade physical field. Background technique [0002] The scientific name of Noni fruit, Morinda citrifolia L., originates in Southeast Asia, Oceania and Australia. This plant mainly grows in subtropical and tropical regions, and is mainly planted in Hainan in my country. Noni fruit is known as "God's gift". Its fruit is rich in polysaccharides, minerals, vitamins, polyphenol flavonoids and other substances. It has been used as traditional medical medicine in Polynesia and other regions for more than two thousand years. In recent years, noni fruit has been confirmed to have anti-oxidation, anti-inflammation, anti-cancer and other health effects, and these effects are significantly related to its polysaccharide composition. [0003] The antioxidative and anticancer activities of polysacc...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P39/06
CPCA61P35/00A61P39/06C08B37/0003
Inventor 曾新安李坚蔡锦林郑志超
Owner SOUTH CHINA UNIV OF TECH
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