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Application of qFAE6 gene in regulating or screening the content of grease component in rice

A technology of component content and gene, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of not knowing the genetic and molecular basis of rice oil

Active Publication Date: 2020-05-12
HUAZHONG AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, there are too few genes and QTLs identified so far, and it is still impossible to know the genetic and molecular basis of rice oil synthesis. Therefore, it is necessary to find the main genes that affect the oil composition and content in rice, and use them as screening markers or genetic manipulation. target

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  • Application of qFAE6 gene in regulating or screening the content of grease component in rice
  • Application of qFAE6 gene in regulating or screening the content of grease component in rice
  • Application of qFAE6 gene in regulating or screening the content of grease component in rice

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Embodiment Construction

[0025] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0026] 1. Sample source

[0027] The rice materials used in the present invention include a natural population composed of 533 cultivated rice varieties and three self-cultivated recombined selfing populations. The natural population was used for genome-wide association analysis, and the three recombinant inbred populations were used for linkage analysis.

[0028] Among them, 533 cultivated rice varieties include 50 Australian rice varieties (aus), 305 indica varieties (indica) and 178 japonica varieties (japonica).

[0029] The three recombinant inbred varieties were derived from the following hybrid lines: Zhenshan 97 / Minghui 63 (ZS97 / MH63), 88B-2 / HD9802S and B805D / H6S.

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Abstract

The present invention relates to application of qFAE6 gene in regulating the content of grease component in rice and application of qFAE6 as a screening marker for the content of grease component in rice for rice breeding. The invention also relates to a method for regulating the content of grease component in rice by genetically manipulating the qFAE6 gene, and a rice hybrid breeding method usingthe qFAE6 gene or mutant thereof as a screening marker.

Description

technical field [0001] The invention relates to the field of rice molecular breeding, more particularly, relates to the application of qFAE6 gene in regulating and screening the content of oil components in rice. Background technique [0002] Lipids in plant seeds are mainly stored in the form of triglycerides. The oil in rice is very important to the storage of rice, the eating quality and the health of consumers, and its oil content is generally 2-4%. The eating quality and palatability of rice are affected by the composition and content of fatty acids in rice. Currently, little is known in the art about the lipid synthesis pathway in rice, Liu et al. demonstrated that OsFAD3 converts linoleic acid (C18:2) to linolenic acid (C18:2). Zaplin et al. confirmed that OsFAD2-1 converts oleic acid to linoleic acid. Some researchers also preliminarily identified several quantitative trait loci (QTL). [0003] However, there are too few genes and QTLs identified so far, and it i...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 何予卿周浩夏朵
Owner HUAZHONG AGRI UNIV