Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mechanical property adjustable hydrogel based on photolytic protein as well as preparation method and application thereof

A technology for mechanical properties and hydrogels, which is applied in the field of hydrogels and their preparation based on tunable mechanical properties of photocleavable proteins, can solve the problems of lack of hydrogel preparation methods and the like

Active Publication Date: 2020-05-15
NANJING UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Currently, there is a lack of a Simultaneous tuning of mechanical properties and biological functions Preparation method and application of hydrogel with adjustable mechanical properties based on photocleavage protein

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mechanical property adjustable hydrogel based on photolytic protein as well as preparation method and application thereof
  • Mechanical property adjustable hydrogel based on photolytic protein as well as preparation method and application thereof
  • Mechanical property adjustable hydrogel based on photolytic protein as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A hydrogel with adjustable mechanical properties based on photocleavage protein of the present invention, the hydrogel with adjustable mechanical property based on photocleavage protein is composed of hydrogel with weakened mechanical properties based on photocleavage protein and photolysis based Hydrogel composition of hydrogels with enhanced mechanical properties of proteins;

[0038] The hydrogel with weakened mechanical properties after illumination is composed of protein Pho-Weak and multi-armed hydrophilic polymers, and the protein Pho-Weak is exposed to sulfhydryl groups in solution and functional group moles of multi-armed hydrophilic polymers The ratio is 1:1;

[0039] The hydrogel with stronger mechanical properties after illumination is composed of protein Pho-Strong and a multi-armed hydrophilic polymer, and the molar ratio of the total sulfhydryl groups of the protein Pho-Strong to the functional group of the multi-armed hydrophilic polymer is 1 :1.

[00...

Embodiment 2

[0048] The difference between Example 2 and Example 1 is that a method for preparing a hydrogel based on photocleavage protein mechanical properties of the present invention with adjustable mechanical properties includes the following steps: In step (1), the construction of the plasmid: protein Pho- Weak changed the 54th proline to cysteine ​​on the basis of the amino acid sequence of the photocleavable protein PhoCl, and added a fragment GGGRSGGC at the end of the entire amino acid sequence, and Pho-Strong was based on the amino acid sequence of the photocleavable protein PhoCl Change the aspartic acid at the 39th position to cysteine, change the proline at the 54th position to cysteine, and treat the gene fragments of the proteins Pho-Weak and Pho-Strong with restriction endonucleases Cloned into the pQE80L vector; the wild-type photocleavable protein is PhoCl, the mutation site is Asp39Cys, Pro54Cys, and the sequence addition fragment is GGGRSGGC from position 243 to positio...

Embodiment 3

[0052] The difference between Example 3 and Example 1 is that a method for preparing a hydrogel based on photocleavage protein mechanical properties of the present invention can be regulated, including the following steps: In step (2), photocleavage protein hydrogel Preparation: The protein obtained in step (1) is mixed with the multi-armed hydrophilic polymer solution, and after a certain period of time under light, the multi-armed hydrophilic polymer is a polymer with a functional group that can react with a sulfhydryl group, such as 4-arm-maleimide-polyethylene glycol, protein Pho-Weak and multi-armed hydrophilic polymer, the molar ratio of protein Pho-Weak exposed sulfhydryl group and multi-armed hydrophilic polymer in solution is 1: 1 Mix to prepare hydrogels with weakened mechanical properties after light irradiation, protein Pho-Strong and multi-armed hydrophilic polymers are mixed with the molar ratio of protein Pho-Strong total sulfhydryl groups and multi-armed hydroph...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
strengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a mechanical property adjustable hydrogel based on photolytic protein as well as a preparation method and application thereof. The invention relates to a preparation method ofhydrogel with adjustable mechanical properties based on photolytic protein. The preparation method comprises the following steps: (1) carrying out site mutation and sequence addition on an original photo-decomposable wild protein sequence to ensure that the protein can have different crosslinking sites before and after illumination while ensuring that the protein can be normally folded and expressed; (2) controlling the number of glue connection points by controlling the molar ratio of a multi-arm hydrophilic polymer to protein crosslinking functional groups in the aspect of glue preparation proportion; and finally, in the aspect of macroscopic regulation and control of mechanical properties, regulating and controlling the change degree of the modulus of the whole hydrogel by controlling the illumination intensity and time to prepare the hydrogel with adjustable and controllable mechanical properties based on the photolytic protein. According to the invention, the mechanical propertiesof the hydrogel can be regulated and controlled according to requirements in time and space; and the hydrogel has no cytotoxicity.

Description

technical field [0001] The invention relates to the technical field of light-controlled hydrogel materials, in particular to a hydrogel whose mechanical properties can be regulated based on photocleavage protein and its preparation method and application. Background technique [0002] Photocleavable proteins have found widespread applications in many fields, including super-resolution imaging, optogenetics, fluorescent sensors, and more recently as switchable crosslinkers for engineering light-responsive hydrogels. Compared with other environment-responsive hydrogels, light-responsive hydrogels have attracted great interest. Because their mechanical properties can be controlled remotely and non-invasively under illumination. [0003] In general, most light-responsive hydrogels are based on the transition between oligomeric and monomeric states of proteins under light. For example, reversible changes between tetramer and monomer of Dronpa145N can lead to reversible changes ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/00C08L71/02C12N5/00C12N5/077
CPCC08J3/075C12N5/0075C12N5/0654C12N2533/30C12N2533/50C12N2506/1346C08J2389/00C08J2371/02Y02A50/30
Inventor 曹毅项董芳吴鑫薛斌秦猛王炜
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com