12 results about "Protein crosslinking" patented technology

The invention relates to the technical field of biological medicines, in particular to a preparation method of protein cross-linked nano affinity microspheres, which comprises the following steps: preparing carboxylated silicon dioxide nano microspheres with the particle size of 500nm by adopting a sol-gel method, mixing tetraethoxysilane and ammonia water according to the volume ratio of 1: (3 + / -1), reacting in an ethanol solution at 40 DEG C for 6 hours, filtering, washing, and drying to obtain the protein cross-linked nano affinity microspheres. And centrifuging to obtain a microsphere precipitate, washing with deionized water for three times to finally obtain a nano microsphere suspension of which the surface is rich in carboxyl, and controlling the solid content to be 10 + / -0.5 mg / mL. The capturing efficiency of the finally prepared protein cross-linked nano affinity microspheres for exosomes reaches 95.0%, the binding capacity reaches 28.5 mu g / mg, the purity of the extracted exosomes is higher than that of an ultracentrifugation method, the biological activity and structural integrity of animal cell exosomes and plant cell exosomes are completely kept, and the protein cross-linked nano affinity microspheres are suitable for large-scale industrial production. The problems that in a traditional exosome extraction method, the recovery rate is low, impurity pollution is serious, exosome activity is damaged, and the application range is limited are solved.

The invention relates to a method for improving the gel quality of skipjack surimi by using vegetable protein, and belongs to the technical field of surimi product processing. The method for improving the gel quality of the skipjack surimi by using the vegetable protein comprises the following steps: sequentially performing air chopping, salt blending, vegetable protein adding, mixing, chopping and moisture adjusting on skipjack meat at 4-10 DEG C to obtain mixed surimi; and filling a mold with the mixed surimi, carrying out heating shaping, and then carrying out ice water cooling to room temperature so as to obtain the surimi gel. The influence of different types and different contents of vegetable proteins on the quality of specific surimi gel is compared, and a proper amount of vegetable proteins are added to promote protein crosslinking so as to improve the strength, texture, water binding capacity and other gel properties of the surimi gel, so that the problems of insufficient strength and poor water binding capacity of the existing skipjack surimi gel are solved, and the skipjack surimi gel has a good application prospect. Therefore, technical support is provided for development of high-quality skipjack surimi products, and the skipjack surimi product has a wide application prospect.

The invention discloses a method for identifying a protein cross-linking mass spectrum peptide fragment pair. Comprising the steps of firstly constructing a peptide fragment vector database, then obtaining and preprocessing spectrogram data, then generating a spectrogram representation sequence, then generating a spectrogram embedding vector, then retrieving candidate peptide fragments in the vector database, and finally determining a target crosslinking peptide fragment and a crosslinking site according to a retrieval result. According to the method, the beneficial effects of remarkably improving the polypeptide segment crosslinking recognition precision and greatly reducing the candidate search space are achieved, and the method is not only suitable for traditional dipeptide crosslinking, but also capable of treating complex crosslinking forms such as tripeptide and tetrapeptide, and has high universality. Meanwhile, a retrieval framework of the vector database supports rapid updating and expansion, and the method is suitable for cross-linked mass spectrum data analysis under different species and different experiment conditions.

A method is provided for making a protein blocking assembly that is formed by reversibly linking a therapeutic protein to a crosslinking material. The protein blocking assembly contains a chemical structure, which is formed between the multiple bonds between the protein and the crosslinking material, that prevents the interaction of the protein blocking assembly with a second protein due to steric and / or static interference. Furthermore, administration methodologies of administering the protein blocking assembly to a subject are provided, where the protein is cleaved from the protein blocking assembly during or after administration.

The present application has an object to provide a novel protein cross-linking method. By allowing both an oxidoreductase such as laccase and a protein deamidase such as protein glutaminase to act on a substrate protein, a cross-linking reaction is promoted.

The cryo-electron microscope sample preparation kit contains an amphipathic protein and a protein crosslinking agent, or an expression vector containing a nucleic acid sequence encoding the amphipathic protein. The kit also includes an ice thickness control molecule to which an amphipathic protein is attached. The amphipathic protein may have an amphipathic α-helix structure and may be either an antifreeze protein or a type I antifreeze protein.

The invention discloses an effective diffusion coefficient model of medium-low salt pickled pigskin based on seepage and fractal double theories and application thereof, through the model constructed based on the double theories, the prediction precision and reliability are remarkably improved, the limitation of a single theory is broken through, and through the combination of the seepage and fractal theories, the effective diffusion coefficient of the medium-low salt pickled pigskin is obtained. The prediction of the model on the effective diffusion coefficient is closer to the actual pickling process; the critical protein crosslinking density is introduced, so that the phenomenon that the effective diffusion coefficient is sharply reduced when the crosslinking density is close to a critical value can be accurately described, and the prediction deviation of a traditional model in a critical state is avoided; according to the method, the geometric fractal dimension of the pigskin is obtained through a box counting method, then the fractal index is determined, the influence of surface area change caused by swelling on a diffusion interface is more scientifically quantified, and the prediction precision of the model in the dynamic process is improved.

This application discloses a method for preparing water-saving surimi gel based on polyphenol-enzyme synergistic crosslinking and its application. The method includes: rinsing surimi raw materials no more than twice; sequentially adding EGCG and TGase to the rinsed surimi, utilizing their synergistic effect to induce protein crosslinking; and then filling and undergoing two-stage heating gelation to obtain the surimi gel. This application significantly improves the breaking strength, elasticity, water-holding capacity, and other textural properties of the surimi gel by introducing synergistic crosslinking of EGCG and TGase into the water-saving rinsing process, successfully overcoming the quality degradation problem caused by reduced rinsing. Simultaneously, the gel exhibits unique digestive-resistant and slow-release properties, delaying protein hydrolysis in the early stages of gastric digestion and ensuring the full release of essential amino acids, thus preserving its nutritional value. This application provides an effective solution for developing high-quality, high-nutrition, and environmentally friendly novel surimi products.

The technical problem of this invention lies in providing a novel protein cross-linking method. This method promotes the cross-linking reaction by allowing both oxidoreductases such as laccase and protein deamidases such as protein glutaminase to act on the substrate protein.

The application belongs to the technical field of genetic engineering, and specifically discloses a glutamine transaminase and application thereof in food. The application obtains a glutamine transaminase mutant with significantly improved enzyme activity and heat resistance by in vitro directional evolution of wild-type glutamine transaminase through error-prone PCR technology. The enzyme activity of the mutant is about 2.4 times that of the wild-type glutamine transaminase, the mutant has strong temperature / pH stability and iron ion tolerance, and can tolerate a salt concentration of 18%. The mutant is used in the preparation of food rich in protein, such as meat products, dairy products, and bean products. The mutant can not only maintain sufficient cross-linking activity under high-temperature heat processing conditions, but also effectively resist the inhibitory effect of Fe 2+ and Fe 3+ in food systems, and still has high catalytic efficiency under conventional or high-iron ion content raw material conditions. With lower enzyme addition amount, the mutant can achieve better protein cross-linking effect, and significantly improve the gel strength, elasticity, water retention and other properties of food.

A method, kits, and devices for performing the method of immediately improving and / or restoring the mechanical integrity and material properties of thermally shrunk collagenous tissue through delivery of a non-toxic protein crosslinker to the tissue. A thermal energy device shrinks the target tissue using controlled application of thermal energy. A non-toxic protein crosslinker is injected and / or topically applied to the target tissue before, during, and / or after thermal shrinkage. A dual applicator may comprise a thermal energy probe and injection needle. The thermal energy probe may apply thermal energy to the target tissue and the injection needle may apply the non-toxic protein crosslinker to the target tissue. A kit for use in performing the method may provide various components used for applying thermal energy and applying the non-toxic protein crosslinker to the target tissue.

The invention provides a high-strength dry electrode, a preparation method thereof and a lithium ion battery, and belongs to the technical field of lithium ion batteries. The high-strength dry-method electrode is prepared by adopting a composite binder, the composite binder is composed of fibrous polytetrafluoroethylene and a polymer serving as a network node, and the polymer serving as the network node is one or a mixture of polyurethane, polyether-ether-ketone, polyamide or a protein cross-linking system. According to the invention, the specific polymer is introduced as a network node and is interspersed and anchored with the PTFE fiber to form a unique three-dimensional double-network structure, so that the tensile strength and flexibility of the dry-method electrode film are remarkably enhanced, the problems of easy breakage, powder falling and the like in the preparation process of the existing dry-method electrode are effectively solved, and the preparation method is particularly suitable for a large-scale roll-to-roll production process. The electrode prepared by the composite binder has excellent mechanical performance and electrochemical performance.