Glutamine transaminase high productive bacteria and its screening method and fermentation method producing glutamine transaminase using said bacterial strain

Abstract

A streptomyce shygeroscopicus (CCTCC No.M203062) able to generate glutamine transaminase wigh high output is extracted from the soil of milk cow farm through plate screening, primary fermenting, flask culture, comparing test with standard color tape, and enzyme activity test. Said glutamine transaminase is prepared by deep fermentation.

Description

technical field [0001] A high-yielding transglutaminase-producing bacterium, a screening method thereof, and a fermentation method for producing transglutaminase using the strain belong to the technical field of bioengineering, in particular to a high-yield transglutaminase-producing Streptomyces hygroscopicus. Background technique [0002] With the gradual improvement of human living standards, the requirements for food are also transitioning from the previous subsistence type to the nutritional type. As one of the most important ingredients in food, protein should be the focus of our attention. Due to the unsatisfactory amino acid composition of many food proteins, or the lack of proper structure and conformation, these proteins lack good functional properties, such as solubility, foaming, emulsification and emulsification stability, and also affect the nutrition of the food itself value. Chemical modification of these proteins to improve their functional properties has ...

AI Technical Summary

Problems solved by technology

For many years, commercial transglutaminase was mainly extracted from animal tissues, but due to the complicated isolation and purification process and the scarcity of sources, it was expensive ($80 / U)