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Application of grape vylhcb4 gene and its encoded protein and gene in the breeding of stress resistant varieties

A grape and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of few species, the biochemical characteristics of the protein encoded by the gene structure, the evolutionary relationship and the stress response research report is not enough, etc., to achieve the enhancement of stress resistance. Effect

Active Publication Date: 2022-07-12
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the most important functional proteins in PSI, the current research on LHCI protein mainly focuses on the capture and transmission of light energy, involving few species, and the gene structure, biochemical characteristics of encoded proteins, evolutionary relationship and stress response. There are few research reports

Method used

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  • Application of grape vylhcb4 gene and its encoded protein and gene in the breeding of stress resistant varieties
  • Application of grape vylhcb4 gene and its encoded protein and gene in the breeding of stress resistant varieties
  • Application of grape vylhcb4 gene and its encoded protein and gene in the breeding of stress resistant varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Analysis of VyLhcb4 gene expression in grapes

[0016] After subculture of Yanshan grape tissue culture seedlings for 16 days, the seedlings with consistent growth, robustness and performance were selected for various adversity treatments.

[0017] Drought treatment: The grape seedlings were pulled out from the medium, placed on filter paper, and exposed to the conditions of room temperature (32±1) °C, relative humidity of 55%, and photoperiod of light for 14h / dark for 10h. 2, 6, 12, 24h sampling.

[0018] Low temperature treatment: The tissue culture seedlings were cultured under the conditions of temperature (4±1)°C, relative humidity of 75%, and photoperiod of light 14h / dark 10h, sampling at 0, 2, 6, 12, and 24 h.

[0019] Salt stress: add 20mL 100mmol·L to the Erlenmeyer flask -1 The NaCl solution was cultured at a temperature of (25±1) °C, a relative humidity of 75%, and a photoperiod of 14h light / 10h dark, and samples were taken at 0, 2, 6, 12, and 24 ...

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Abstract

The invention relates to the application of grape VyLhcb4 gene and its encoded protein and gene in the breeding of stress-resistant varieties, and belongs to the technical field of plant genetic engineering. In the present invention, the transgenic technology driven by a strong promoter (cauliflower mosaic virus 35S promoter) is used to transfer the overexpression vector of the VyLhcb4 gene of Yanshan grape into Arabidopsis thaliana, thereby obtaining transgenic Arabidopsis thaliana plants; Compared with Arabidopsis plants transformed with empty vector, overexpression of VyLhcb4 gene led to the accumulation of stress-related substances and the expression of stress-related genes in transgenic Arabidopsis, and the stress resistance of transgenic plants was enhanced. Therefore, the grape VyLhcb4 gene and its recombinant expression vector can be used for plant stress-resistant variety breeding.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the application of grape VyLhcb4 gene and its encoded protein and gene in the breeding of stress-resistant varieties. Background technique [0002] The light-harvesting chlorophyll a / b-binding protein is an important functional protein in the photosynthetic system. It is located on the thylakoid membrane of the chloroplast and combines with the pigment to form a light-harvesting pigment protein complex. The complex can capture light energy and quickly transfer the light energy to the reaction center of PSI and photosystem II, causing a photochemical reaction to convert the light energy into chemical energy. In higher plants and green algae, the PSI complex consists of the PSI core complex and the light-harvesting pigment protein complex I. Eukaryotic PSI complexes include a series of light-harvesting chlorophyll a / b-binding proteins or LHCIs, which bind to abou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/20A01H6/88
CPCC07K14/415C12N15/8273
Inventor 余义和刘风英张海涛李敏张贺程郭大龙杨英军张国海
Owner HENAN UNIV OF SCI & TECH
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