Preparation method and application of modified silica gel for extracting urine protein

A urine protein and silica gel technology, which is applied in the field of protein extraction and purification, can solve the problems of complicated urine protein recovery and separation operations, inability to be widely promoted, and low collection efficiency, so as to simplify the extraction process and workload, and achieve high utilization of silica gel , good adsorption effect

Active Publication Date: 2020-05-22
江西浩然生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional recovery and separation of urine protein is more complicated. The traditional recovery method is to collect and summarize the urine first, then perform adsorption extraction or precipitation operation, and there are disadvantages such as environmental pollution, low collection efficiency and high processing cost, making it unable to be widely promoted.

Method used

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  • Preparation method and application of modified silica gel for extracting urine protein
  • Preparation method and application of modified silica gel for extracting urine protein
  • Preparation method and application of modified silica gel for extracting urine protein

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Such as figure 1 As shown, the present invention provides a method for preparing modified silica gel for extracting urinary protein. The modified silica gel is amino polystyrene bonded silica gel. The preparation method of the modified silica gel is specifically:

[0037] Step 101: react the silica gel with nitric acid, then react with concentrated hydrochloric acid, wash and dry the product to obtain activated silica gel.

[0038] Wherein, in combination with specific implementation scenarios, the washing and drying of the product may be as follows: the product is washed to be free of chloride ions, and then dried at 120-150°C.

[0039] Step 102: Add the activated silica gel, vinyltriethoxysilane and heavy fumed toluene into a three-necked flask, pass through an inert gas for protection, and then pass through ammonia gas for reaction, purify and dry the product to obtain the precursor I (vinyl ethoxylated silicon-bonded silica gel).

[0040] Step 103: Add the precurs...

Embodiment 2

[0045] In a specific implementation scenario, the aminopolystyrene-bonded silica gel for extracting urinary protein can be prepared by the following method:

[0046] Step 201: Add 1:1 (V / V) nitric acid to the silica gel and reflux for 6-8 hours. After the silica gel settles, discard the nitric acid waste liquid in the upper layer, add concentrated hydrochloric acid, reflux for 6-8 hours, wash until there is no chloride ion, 120- Dry at 150°C to obtain activated silica gel.

[0047] Step 202: Add 50 g of activated silica gel, 50 ml of vinyltriethoxysilane and 20 ml of heavy fumed toluene in step 201 into a three-necked flask, pass argon gas for 30 minutes, then pass ammonia gas and stir at the same time, and react at room temperature for 12-14 Hour. The product was suction filtered, washed several times with ethanol, transferred to a Soxhlet extractor and extracted with absolute ethanol for 10 hours, and then dried in vacuum at 40-50°C to obtain precursor I (vinyl ethoxy silic...

Embodiment 3

[0052] Such as figure 2 As shown, the present invention provides a method utilizing amino polystyrene bonded silica gel to extract urine protein, comprising:

[0053] Step 301: Adsorb urine protein after contacting amino polystyrene bonded silica gel with urine.

[0054] Step 302: The urine protein is collected by eluting from the adsorbed modified silica gel.

[0055] Among them, in combination with specific implementation scenarios, amino polystyrene bonded silica gel can be packed into a cloth bag sewn with cotton gauze, and the cloth bag is sealed and placed in a urinal or urinal, and after contact with urine, urine protein is absorbed; it can be used in the morning Place it, take it back at night, and carry out elution treatment on the modified silica gel after adsorption.

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Abstract

The invention relates to a preparation method and application of modified silica gel for extracting urine protein. According to the invention, silica gel is modified into amino polystyrene bonded silica gel; urine proteins such as urokinase, human urokininogenase and the like in urine can be directly adsorbed by utilizing the modified silica gel, then the urine proteins are sequentially eluted byadopting different elution conditions according to the properties of different isoelectric points of the different urine proteins, and finally effective adsorption of the urine proteins and separationand enrichment of the different urine proteins can be realized; and the silica gel is high in utilization rate, good in adsorption separation effect, small in environmental pollution, convenient to use, low in cost and suitable for large-scale industrial production, and urine does not need to be collected and a urinal does not need to be transformed.

Description

technical field [0001] The invention belongs to the technical field of protein extraction and purification, and in particular relates to a preparation method and application of modified silica gel for extracting urinary protein. Background technique [0002] Male urine contains urinary proteins such as urokinase and human urinary kininogenase, which can be recovered and processed into corresponding raw materials and preparations. Urokinase is suitable for the clinical treatment of various thrombosis and myocardial infarction and other diseases. It is also used to prevent thrombosis after artificial heart valve surgery, maintain vascular catheterization and thoracic and pericardial drainage tubes, etc.; human urinary kallikreinase It is suitable for the treatment of diseases such as hypertension, microcirculation disorder, peripheral and cerebrovascular obstruction. It can be seen that these urinary proteins have high medicinal and economic value, therefore, it is necessary ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30C12N9/64C12N9/72
CPCB01J20/261C12N9/6445C12N9/6462C12Y304/21035C12Y304/21073
Inventor 胡著阳耿卫锋熊坚坚李菊根戴曼庆
Owner 江西浩然生物制药有限公司
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