Apparatus for dissection of Drosophila larval wing buds

Abstract

The invention provides a device for dissecting wing buds of fruit fly larvae, which belongs to the field of insect dissection, and aims to solve the problems of time-consuming, labor-intensive and low dissecting efficiency in the current method. Including the outer barrel, the inside of the outer barrel is coaxial with the inner barrel, and there are several holes on the surface of the inner barrel. Each hole is correspondingly equipped with a thimble. One end of the thimble extends into the hole, and the other end of the thimble is connected to the top sheet. A spring is sleeved, one end of the spring is connected with the inner wall of the inner barrel, the other end of the spring is connected with the top plate, a fruit fly stopper is provided at the end of the hole close to the outer barrel, a cylindrical blade is arranged on the top of the inner barrel, and the surface of the blade is provided with a The holes correspond to the round holes one by one. There is a coaxial top sheet pusher inside the blade. Both the blade and the top sheet pusher are connected to the top of the outer barrel through a fixed bracket. The bottom of the outer barrel is equipped with an outer barrel connected to the outer barrel. Temperature control system, the bottom of the inner barrel is connected with the inner barrel temperature control system.

Description

technical field [0001] The invention relates to the technical field of insect anatomy, in particular to a device for dissecting wing buds of fruit fly larvae. Background technique [0002] Drosophila is a classic model organism. Drosophila larval wing buds are the research object, which provides a good research model for organ development. The structure of the Drosophila larval wing bud is relatively simple, and it is located about 1 / 3 from the head of the larva. The study of wing buds often needs to dissect the wing buds first, and determine the expression of genes by immunohistochemical staining. [0003] The current method for dissecting the wing buds of Drosophila larvae is as follows: first cut the 3rd instar larvae of Drosophila progeny into two sections with tweezers, and discard the second half of the larvae. Then, use the tweezers to hold the epidermis of the end of the first half of the larva, and the other tweezers against the head of the larva, and turn the in...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems of time-consuming and labor-intensive and low dissection efficiency in the current method of dissecting the late wing buds of Drosophila larvae, the present invention provides a device for dissecting the wing buds of Drosophila larvae

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