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Micro-fluidic chip sample adding device and testing method

A microfluidic chip and sample loading device technology, applied in the direction of analysis materials, instruments, etc., can solve the problems of restricting automatic implementation and introducing air bubbles, and achieve the effects of easy implementation and promotion, prevention of air bubble introduction, and low cost

Pending Publication Date: 2020-06-16
厦门大学附属翔安医院
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  • Abstract
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  • Claims
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Problems solved by technology

As a result, air bubbles are often introduced during the sample loading process of the microfluidic chip, which is still an important reason restricting its automatic implementation.

Method used

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  • Micro-fluidic chip sample adding device and testing method

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Experimental program
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Embodiment Construction

[0023] Attached below figure 1 The technology will be further described with specific examples to help understand the content of the present invention.

[0024] A microfluidic chip sampling device, comprising a conical sampling pool 1, the bottom of the sampling pool 1 is in close contact with the chip inlet 11; A liquid discharge pipe 3 is provided on the top; a pressure discharge pipe 4 and a sampling main pipe 5 are provided on the pressure piece 2, and the sampling main pipe 5 is respectively connected with the antibody collection pipe, the buffer collection pipe and the air pump 7; the liquid discharge pipe 3, the pressure relief pipe 5 Tube 4, outlet of air pump 7, antibody collection tube, buffer solution collection tube, and chip outlet 11 are all provided with electromagnetic valves; at least one antibody collection tube is provided, and this embodiment provides multiple, and all are provided with battery valves, which can be determined according to the experiment It...

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Abstract

The invention discloses a microfluidic chip sample adding device, and the device comprises a conical sample adding pool, wherein the bottom of the sample adding pool is in close contact with a chip inlet; a pressing part is arranged at the top of the sample adding pool, a small hole is formed in the bottom, and a liquid discharging pipe is arranged on the side wall; a pressure discharging pipe anda sample adding main pipe are arranged on the pressing part, and the sample adding main pipe is connected with the antibody collecting pipe, the buffer solution collecting pipe and the air pump respectively. The device has the advantages that the sample adding requirements of large-capacity blood samples and trace antibody reagents can be considered, bubbles in a pipeline can be automatically discharged in front of a chip inlet, and the experiment accuracy and the automation degree are improved.

Description

technical field [0001] The invention belongs to the field of microfluidic chips, and in particular relates to a sample adding device which is connected with a chip inlet and can automatically complete gas-liquid conversion and reagent replacement. Background technique [0002] Due to the advantages of high efficiency, portability and integration, microfluidic technology has shown great application prospects in mass spectrometry, antigen detection, cell culture, cell sorting, drug analysis, etc. High-level detection and analysis items are changing to portability, low cost, and bedside. However, a detection item often requires the sequential addition of multiple chemical reagents for incubation and reaction, which involves removing residual reagents in the inlet pipeline and flushing or replacing with new reagents after a reaction is completed. Checking patents and literature shows that there are many ways to replace reagents. Among them, manual sample addition can flexibly f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N35/10
CPCG01N35/00G01N35/00584G01N35/1016G01N35/1004G01N35/10G01N2035/00237G01N2035/00277G01N2035/1034
Inventor 彭志海王杰唐华美陈翔韩超李洋
Owner 厦门大学附属翔安医院
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