Composition and application of borneol combined with neurotrophic factors
A technology of ciliary neurotrophy and borneol, which is applied in the direction of drug combination, active ingredients of hydroxyl compounds, drug delivery, etc., can solve the problems of no retinal disease treatment, achieve good drug compliance, increase safety and convenience, and promote distribution effect
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Embodiment 1
[0038] Embodiment 1 Borneol CNTF eye drops preparation
[0039] (1) Gel matrix eye drop sample preparation: Weigh 1.0 g of carboxymethylcellulose sodium (CMC-Na), add 50 g of water for injection, stir to make it evenly dispersed, leave it at room temperature overnight, add the remaining 49 g of water for injection, Stir for 5-10 min, and let stand to obtain 1% CMC-Na gel matrix for later use.
[0040] (2) Weigh 1.336g of polyoxyethylene hydrogenated castor oil 40 (RH40), 0.334g of polyethylene glycol 400 (PEG400), stir for 10min and mix evenly for later use;
[0041] (3) Weigh 0.025g of borneol and add it to step (2), stir to make it completely dissolve;
[0042] (4) Weigh 200 μg of CNTF and dissolve it in 2 mL of phosphate-buffered saline (PBS), and set aside;
[0043] Add (3) and (4) into 10g of 1% CMC-Na gel, stir for 10min to disperse evenly, then add 86.105g of 1%CMC-Na several times in small amounts, stir to disperse evenly, and adjust the pH If the value is 6.0-8.0, ...
Embodiment 2
[0045] Example 2 Effects of Different Concentrations of Borneol on CNTF Retinal Distribution
[0046] Experimental animal New Zealand rabbits were randomly divided into 6 groups, normal saline group, borneol eye drops group, CNTF eye drops group, different concentrations of borneol CNTF eye drops groups (borneol concentration 0.025%, 0.05%, 0.1%), respectively. 50 μL of the corresponding eye drops were instilled in the eyes, before the administration (0h) and 15 minutes, 30 minutes, 1 hour, and 2 hours after the administration, 5 rabbits were randomly selected in each group to be killed and the retinas were taken, and the enzyme-linked immunosorbent assay was used to (ELISA) method was used to detect the content of CNTF in retinal tissue, and each sample was set up with three duplicate wells, and the average value was taken. The results showed that after administration for 15 minutes, the CNTF content determination results of the borneol group and the blank group were 2.8±0.0p...
Embodiment 3
[0049] Example 3 Effect of Borneol CNTF Eye Drops on Retinal Function in Rats with Retinal Degeneration
[0050] Using 40mg / kg sodium iodate tail vein injection to induce retinal degeneration rat model, the rats after modeling were randomly divided into normal saline group, borneol eye drop group, CNTF eye drop group, borneol CNTF eye drop group, Normal SD rats were fed routinely as a control, and the drug was administered 4 times a day, 10 μL each time. After 14 days of administration, the retinal function was detected by electroretinogram (ERG). Rats were dark-adapted to general anesthesia combined with local anesthesia and mydriasis. Animal warmers were used. Rat-specific circular corneal electrodes were used to elicit retinal integrated potentials with standard flash stimulation. by retinal electricity Figure five Item measurement, record value. Including scotopic ERG, maximal mixed response, oscillatory potentials (OPs), photopic condition ERG, flicker light ERG. turn...
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