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Nucleic acid for inhibiting STAT3 gene expression, pharmaceutical composition with same nucleic acid and application thereof

A gene expression and nucleotide technology, applied in the field of siRNA, can solve the problem of lack of sufficient understanding of the degradation process of siRNA

Pending Publication Date: 2020-07-07
SUZHOU RIBO LIFE SCIENCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, those skilled in the art have done a lot of research on siRNA. However, the degradation process and mechanism of siRNA in blood are still insufficiently understood. Experiment to verify the process

Method used

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  • Nucleic acid for inhibiting STAT3 gene expression, pharmaceutical composition with same nucleic acid and application thereof
  • Nucleic acid for inhibiting STAT3 gene expression, pharmaceutical composition with same nucleic acid and application thereof
  • Nucleic acid for inhibiting STAT3 gene expression, pharmaceutical composition with same nucleic acid and application thereof

Examples

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preparation example 1

[0263] Preparation Example 1 This example illustrates the siRNA provided by the present disclosure and the control siRNA

[0264] In this preparation example, the siRNA sequences in Table 1 were synthesized.

[0265] In siRNA synthesis, unless otherwise specified, nucleoside monomers refer to modified nucleoside phosphoramidite monomers used in solid phase synthesis of phosphoramidites according to the type and sequence of nucleotides in the siRNA to be prepared (modified RNA phosphoramidites). Phosphoramidite solid phase synthesis is a method used in RNA synthesis well known to those skilled in the art. The nucleoside monomers used in this preparation example are all commercially available.

[0266] In this preparation example, the siRNAs listed in Table 1 below were obtained by the conventional solid-phase phosphoramidite method. Use DEPC water (purchased from Amresco, product number E174) to dissolve equimolar sense strand and antisense strand respectively, heat to 70-95...

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Abstract

The invention provides siRNA for inhibiting STAT3 gene expression. The siRNA comprises a sense strand and an antisense strand, wherein the sense strand comprises a nucleotide sequence 1, the antisensestrand comprises a nucleotide sequence 2, the length of the nucleotide sequence 1 and the length of the nucleotide sequence 2 are equal to 19 nucleotides, the nucleotide sequence 1 and the nucleotidesequence 2 are at least partially reversely complementary to form a double-strand region, the nucleotide sequence 2 is at least partially reversely complementary to a first nucleotide sequence, and the first nucleotide sequence is a nucleotide sequence which is the same as the nucleotide sequence 2 in length in STAT3 mRNA. According to the direction from the 5' tail end to the 3' tail end, the nucleotides at the 7, 8 and 9 sites of the nucleotide sequence 1 are fluoro-modified nucleotides, and the nucleotides at the 2<nd>, 6, 14 and 16 sites of the nucleotide sequence2 are fluoro-modified nucleotides. The siRNA and a pharmaceutical composition containing the siRNA have good activity of inhibiting STAT3 gene expression, thereby having a good effect of inhibiting tumors.

Description

technical field [0001] The present disclosure relates to a siRNA, a pharmaceutical composition containing the siRNA and their uses. Specifically, the present disclosure relates to an siRNA for inhibiting STAT3 gene expression, a pharmaceutical composition containing the siRNA as an active ingredient, and the preparation of the siRNA and the pharmaceutical composition for preventing and / or treating the overexpression of STAT3 Application of medicines related to pathological conditions or diseases. Background technique [0002] Signal transducer and activator of transcription 3 (STAT3) is a special transcription factor. Current studies have shown that there are abnormal signal transduction and abnormal activation of STAT3 in glioma and many other malignant tumor cells . The up-regulation of STAT3 can induce the proliferation of tumor cells, and its down-regulation can promote the apoptosis of tumor cells. It is a key point in the activation of various growth factors or cytok...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K31/713A61P35/00
CPCC12N15/1135A61K31/713A61P35/00C12N2310/141
Inventor 张鸿雁高山康代武
Owner SUZHOU RIBO LIFE SCIENCE CO LTD