Glycan preparations for the treatment of infection
A preparation, glycan technology, applied in the field of glycan preparations for the treatment of infections, can solve problems such as no treatment options yet
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example 1
[0623] Example 1. Glycan preparations
[0624] To a round bottom flask equipped with an overhead stirrer and a jacketed short-circuit condenser add one or more monosaccharides or disaccharides and 3%-20% by dry weight of one or more e.g. like in the US Catalysts such as acid, ion, ion / acid containing catalysts described in Patent No. 9,079,171 and WO 2016 / 007778 (these patents are incorporated herein by reference in their entirety). Water or other compatible solvent (zero or 10 equivalents) was added to the dry mixture and the slurries were combined at approximately 100 rpm using a paddle sized to match as close as possible to the contour of the selected round bottom flask. The mixture was then heated to 80°C-185°C. Once the solid has achieved a molten state, the vessel is placed under a vacuum pressure of 10-1000 mbar. The reaction was stirred for 30 minutes to 8 hours with continuous removal of water from the reaction. The progress of the reaction was monitored by HPLC. ...
example 2
[0632] Example 2. Purification
[0633] Dissolve oligosaccharides and polysaccharides in deionized water to a final concentration of 25-50 Brix. The material was then exposed to at least 2 mass equivalents of Dowex Monosphere 88 ion exchange resin. Exposure can be performed by vortexing in the flask at 120-170 rpm or by filtering through a packed column of wet slurry, as long as the residence time is sufficient for the solution to achieve a final pH of 3 to 5. The oligomer solution was isolated by filtration (as in the case of vortex reactions) or elution (as in the case of column filtration), and the process was repeated in a similar manner with Dowex Monosphere 77 ion exchange resin until the solution pH was higher than 5.5. Finally, the solution was exposed to Dowex Optipore SD-2 sorbent decolorizing resin until the solution was sufficiently clear and filtered through a 0.2 micron filter to remove residual resin and resin fines. The final solution was then concentrated t...
example 3
[0634] Example 3. Small-scale high-throughput preparation
[0635] Oligomers and polymers were synthesized in parallel in 24-, 48- or 96-well plates or in similarly sized arrays of 1 dram vials housed in aluminum heating blocks. In this example, all liquid transfers were performed by a programmable robot or manually using calibrated pipettes. Add 20%-100% by dry weight to each vial or well of one or more catalysts such as those described in US Patent No. 9,079,171 and WO 2016 / 007778, e.g. catalyst. Place the plate or heating block uncovered in a vacuum oven and heat to 50°C-150°C under a vacuum of 10-800 mbar. The oven vacuum pump is protected by a two-stage condenser consisting of a circulating coolant trap followed by a dry ice / acetone trap. The plate or block is heated at elevated temperature and reduced pressure for 30 minutes to 6 hours without agitation. After a pre-set period of time, the oven is vented to atmospheric pressure, the plate or block is cooled to room t...
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