Hybrid mesenchymal stem cell exosome drug delivery system as well as preparation method and application thereof

A mesenchymal stem cell and delivery system technology, applied in the field of hybrid mesenchymal stem cell exosome drug delivery system and its preparation, can solve the problems of tumor drug resistance, poor prognosis of tumor treatment, etc. To improve the efficiency and improve the effect of tumor targeting inhibition

Inactive Publication Date: 2020-07-28
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The prognosis of cancer treatment is poor,

Method used

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  • Hybrid mesenchymal stem cell exosome drug delivery system as well as preparation method and application thereof
  • Hybrid mesenchymal stem cell exosome drug delivery system as well as preparation method and application thereof
  • Hybrid mesenchymal stem cell exosome drug delivery system as well as preparation method and application thereof

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preparation example Construction

[0057] A method for preparing a hybrid mesenchymal stem cell exosome drug delivery system as described above, the steps are as follows:

[0058] S1 Dissolve cholesterol components and phospholipid materials in an organic solvent at a weight ratio of 5-20:80-95, then add fat-soluble antineoplastic drugs, and bathe in a water bath of 35-55°C to dissolve the cholesterol components and phospholipid materials evenly;

[0059] Under the condition of S2 water bath, rotate and vacuum to remove the organic solvent, so that a honeycomb film is formed on the inner wall of the container, and place it in a vacuum drying oven for 8-12h;

[0060] S3 Take the film obtained in step S2, add a pH buffer solution in which a water-soluble antitumor drug is dissolved, and rotate in a water bath at 40-60° C. to completely dissolve the film;

[0061] S4 Ultrasound or homogenization to prepare drug-loaded liposomes with a median diameter of 60-200 nm;

[0062] S5 uses the preparation technology of pH...

Embodiment 1

[0074] Example 1 Preparation of tirapazamine-hydralazine dual-loaded hybrid mesenchymal stem cell exosomes

[0075] (1) Preparation of exosome stock solution

[0076] Take 1ml of fresh animal mesenchymal stem cell sample into a cell culture bottle, add DMEM / F12 medium containing 10% fetal bovine serum, and culture in a constant temperature incubator at 37°C and 5% CO2; respectively, at 24, 48 and 72 hours Change the medium of the culture bottle completely, and discard non-adherent cells such as red blood cells and adipocytes; change the medium every three days, perform primary culture for 10-15 days, and conduct the first passage, and observe the growth of adherent mesenchymal stem cells after 4-7 days When reaching 80-90%, subculture again; get the bone marrow mesenchymal stem cells of the 3rd-6th generation ( figure 1 ), collected cell supernatant 100ml, divided into centrifuge tubes, centrifuged at 300×g for 10min, took the supernatant, centrifuged at 2000×g for 10min, too...

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Abstract

The invention relates to a hybrid mesenchymal stem cell exosome drug delivery system. The drug delivery system is constructed by hybridizing and fusing mesenchymal stem cell exosomes and liposomes. The system has passive targeting and active targeting of tumors, the tumor targeting efficiency is remarkably improved, the toxic and side effects of the whole body are reduced, and the system can be applied to the preparation of drugs, especially anti-cancer drugs.

Description

technical field [0001] The invention belongs to the field of medicine technology, and relates to a tumor-targeted drug delivery system, in particular to a hybrid mesenchymal stem cell exosome drug delivery system and its preparation method and application. Background technique [0002] According to the latest statistics from the International Agency for Research on Cancer (IARC), in 2018, there were 18.1 million new cancer cases and 9.6 million deaths in the world, and the incidence and mortality of cancer in my country are the highest in the world. In tumor chemotherapy, the targeting efficiency of anticancer drugs is not high, and strong systemic toxic and side effects are the most prominent problems, even the direct or indirect cause of one-third of the death of patients, so it is necessary to make full use of the relationship between tumors and normal tissues Differential, tumor-specific therapy with reduced toxicity to normal tissues. [0003] Liposome is an artificial...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/46A61K45/06A61K31/502A61K31/53A61P35/00
CPCA61K9/1277A61K9/1271A61K47/46A61K45/06A61K31/502A61K31/53A61P35/00A61K2300/00
Inventor 李明媛郁彭李双双李媛郭娜杜春阳
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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