Application of ctbp2 in the preparation of diagnostic products for hepatocellular carcinoma and intrahepatic cholangiocarcinoma

A technology for hepatocellular carcinoma and inner bile duct, which is applied in the field of biomedicine and can solve the problems of specificity and sensitivity

Active Publication Date: 2022-07-15
NANTONG TUMOR HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Each marker has been reported to have its own drawbacks, including sensitivity and specificity

Method used

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  • Application of ctbp2 in the preparation of diagnostic products for hepatocellular carcinoma and intrahepatic cholangiocarcinoma
  • Application of ctbp2 in the preparation of diagnostic products for hepatocellular carcinoma and intrahepatic cholangiocarcinoma
  • Application of ctbp2 in the preparation of diagnostic products for hepatocellular carcinoma and intrahepatic cholangiocarcinoma

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Embodiment 1

[0016] In this example, healthy people were used as controls, and hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) patients were used as research objects to verify the exact role of CtBP2 in the differential diagnosis of primary liver cancer.

[0017] Liver tissues from healthy individuals and patients were fixed in 10% buffered formalin and routinely processed for paraffin embedding. Immunohistochemical staining was performed on a Dako Omnis automated stainer according to standard procedures.

[0018] Antibodies used are as follows: HepPar-1 (Beijing Zhongshan Golden Bridge Company, catalog number ZM-0131, dilution 1:200, clone number OCH1E5), ARG1 (Zeta Corporation, USA, catalog number Z2335, dilution 1:200, clone number ZM30) , CK19 (Beijing Zhongshan Golden Bridge Company, catalog number ZM-0074, dilution 1:800, clone number UMAB2), CtBP2 (Abcam, USA, catalog number ab128871, dilution 1:300, clone EPR7611[B]).

[0019] Immunohistochemical staining ...

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Abstract

The invention discloses an application of using CtBP2 as a marker in the preparation of diagnostic products for hepatocellular carcinoma and intrahepatic cholangiocarcinoma, and belongs to the technical field of biomedicine. The research results of the present invention show that most high-moderately differentiated HCCs are negative for CtBP2, and only a few poorly differentiated HCCs are positive; while in ICC, CtBP2 is diffusely strongly positive. Therefore, CtBP2 can be used as a marker to prepare related products for the identification and diagnosis of hepatocellular carcinoma and intrahepatic cholangiocarcinoma, thereby providing a new method for the identification, diagnosis or drug efficacy evaluation of hepatocellular carcinoma and intrahepatic cholangiocarcinoma. objective indicators.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of CtBP2 as a marker in the preparation of diagnostic products for hepatocellular carcinoma and intrahepatic cholangiocarcinoma. Background technique [0002] Hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) are the two most common pathological types of primary liver cancer. HCC often shows hepatocyte differentiation, whereas ICC shows bile duct epithelial differentiation. In addition, there is a relatively rare type of primary liver cancer called mixed hepatocellular carcinoma-cholangiocarcinoma (CHC), which often shows both hepatocyte and bile duct epithelial differentiation. In most cases, pathologists can make the correct pathological diagnosis of primary liver cancer by combining clinical manifestations, imaging, and routine hematoxylin-eosin (H&E) stained sections. However, the differential diagnosis of ICC and HCC r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/68G01N33/57438G01N33/57484
Inventor 何松吴雅珣尹海兵缪小兵
Owner NANTONG TUMOR HOSPITAL
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